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Amazon Shop: ½ Pint Jars

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OfflineVagrant
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Registered: 10/03/04
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7 out of 22! WTF!
    #3246663 - 10/12/04 11:10 AM (13 years, 11 days ago)

OK, here's the deal. About a month ago, I made up 22 half-pint jars and PCed them at 15psi for 60 minutes. Two weeks went by, no contams, so I inocced. The jars are 90-95% colonized. However, 15 of them show small amounts of green in the uncolonized parts. That sucks, I sterilized the room and sterilized the needle between jars. My syringes came from a reputable shroomery sponsor. Should I wait to see if the mycelium takes over or just keep the 7 that show no signs of contamination? What could possibly be going wrong? Could waiting too long after PCing to inoculate be my problem? Thanks.


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OfflineKoOs
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Re: 7 out of 22! WTF! [Re: Vagrant]
    #3246675 - 10/12/04 11:21 AM (13 years, 11 days ago)

Quote:

Vagrant said:
OK, here's the deal. About a month ago, I made up 22 half-pint jars and PCed them at 15psi for 60 minutes. Two weeks went by, no contams, so I inocced. The jars are 90-95% colonized. However, 15 of them show small amounts of green in the uncolonized parts. That sucks, I sterilized the room and sterilized the needle between jars. My syringes came from a reputable shroomery sponsor. Should I wait to see if the mycelium takes over or just keep the 7 that show no signs of contamination? What could possibly be going wrong? Could waiting too long after PCing to inoculate be my problem? Thanks.




Why did you INOCULAT 2 weeks later?

You should INOCULAT when the jars have cooled.


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OfflineVagrant
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Re: 7 out of 22! WTF! [Re: KoOs]
    #3246683 - 10/12/04 11:36 AM (13 years, 11 days ago)

I read on fanaticus.com that you could test the jars by letting them sit before inoculation to see if you are PCing correctly. I'm not going to do that again. Do yo think that could've been my problem?


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OfflineHamurabi
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Re: 7 out of 22! WTF! [Re: Vagrant]
    #3246688 - 10/12/04 11:40 AM (13 years, 11 days ago)

certainly


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OfflineAnnoA
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Re: 7 out of 22! WTF! [Re: Vagrant]
    #3246694 - 10/12/04 11:43 AM (13 years, 11 days ago)

If the contamination starts directly at the inoculation points and shortly after inoculation, then the syringes(either dirty needle or diry content) are to blame. Otherwise not.


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Offlineshadow69playa
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Re: 7 out of 22! WTF! [Re: Anno]
    #3246718 - 10/12/04 12:02 PM (13 years, 11 days ago)

heres what COULD have happened.

when u let the jars sit for the 2 weeks.. were the holes covered? contams may have entered through there.

also what kind of temp etc. conditions were the jars in for the 2 weeks? was it a controlled enviroment? or were they jus sittin in a box or what? those could have also been factors in it.


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OfflineVagrant
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Re: 7 out of 22! WTF! [Re: shadow69playa]
    #3246729 - 10/12/04 12:07 PM (13 years, 11 days ago)

I had tape covering the holes. The jars were in a box probably at about room temp.


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InvisibleSam1912
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Registered: 09/23/04
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Re: 7 out of 22! WTF! [Re: shadow69playa]
    #3246734 - 10/12/04 12:08 PM (13 years, 11 days ago)

yeah, I keep my jars under observation, too.. but only for 2 days.

Sam


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OfflineVagrant
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Re: 7 out of 22! WTF! [Re: Sam1912]
    #3246767 - 10/12/04 12:23 PM (13 years, 11 days ago)

I think on my next run, I'll omit the observation stage and inoculate right after cooling. I'm also making a tub in tub incubator that should be ready. Hopefully the myc will outgrow any contams.
So tell me, should I throw out the jars that show a small amount of green or see if the myc out competes the contams? They are about 90-95% colonized.


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InvisibleSam1912
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Registered: 09/23/04
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Re: 7 out of 22! WTF! [Re: Vagrant]
    #3246774 - 10/12/04 12:27 PM (13 years, 11 days ago)

I'd say yeah. Don't mess with contam. It's probably worse inside anyways. If you decide to disect it, do it outside. Don't mess your pad up with contam. Good luck.

Sam


--------------------
Protect your civil rights!  End drug prohibition.  And if you don't care about your civil rights, protect mine!

If you want a rating from me, please PM me.  For those really newbies, don't expect an answer back, but you can try me anyways.


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InvisibleMagashM
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Re: 7 out of 22! WTF! [Re: Vagrant]
    #3247212 - 10/12/04 02:32 PM (13 years, 11 days ago)

Letting the jars sit isn't causing the contams. You should be able to let em sit for a year. They can't contam if there isn't some kind of contam spore in there.


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Amazon Shop: ½ Pint Jars

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