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Amazon Shop for: ½ Pint Jars, Rye Grain, Wild Bird Seed

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InvisibleGnuBobo
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My f'ing Karo jars keep comin' up clouds
    #3241317 - 10/11/04 02:04 AM (12 years, 1 month ago)

    I've made like 6 karo jars now.  Ok, granted, three of them used a rye berry with myc to innoc.  THose looked all right, not cloudy, but contamed eventually and it was 4th gen. myc anyway.
    But now I just tried to make up two more with a vendor syringe.  One is cloudy (I just injected it with bleach so I can empty it tomorrow).  THe other is not looking good.  This vendor syringe is colonizing the quarts I used it to innoc. all right.  Though this is happening quite slowly. 
    I boiled the karo jars for 30 minutes; used boiling water to mix in with the karo.  I tbsp. per 100ml.  Then I put the karo water in the boiled jars, put the tyvek and lids on, pc'd for 30 minutes.  What's messed is that I innoced in a glovebox, and I didn't have any contams with the syringe for the grain.  WTF?  Any ideas?  I'd like to see a successful karo, just once.  I'm all saddy and blue now. :sad:

Thanks for any help!

GB


--------------------
Jerry Garcia. JERRY GARCIA! JERRY GARCIA!!!!


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Offlineatomic1
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Re: My f'ing Karo jars keep comin' up clouds [Re: GnuBobo]
    #3241528 - 10/11/04 02:53 AM (12 years, 1 month ago)

I dunno, sounds like you need to refer to fastfreds guide to distinguishing the difference between clouds and cloudy. :lol:
No offense, its just funny if you read that thread a while back.  So are you saying you got a cloudy jar but not a jar with clouds of mycelium?


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Offlinescatmanrav
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Re: My f'ing Karo jars keep comin' up clouds [Re: GnuBobo]
    #3241529 - 10/11/04 02:54 AM (12 years, 1 month ago)

Karo looks _slighty_ cloudyish. Are you sure its not succesful and you are just thinking its contams since you mentioned not seeing a successful karo jar?



--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


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Offlineatomic1
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Re: My f'ing Karo jars keep comin' up clouds [Re: scatmanrav]
    #3241553 - 10/11/04 02:59 AM (12 years, 1 month ago)

Quote:

scatmanrav said:
Karo looks _slighty_ cloudyish. Are you sure its not succesful and you are just thinking its contams since you mentioned not seeing a successful karo jar?




Thats what i'm thinking.


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InvisibleGnuBobo
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Re: My f'ing Karo jars keep comin' up clouds [Re: atomic1]
    #3241579 - 10/11/04 03:04 AM (12 years, 1 month ago)

Well, I started getting dark growth on the sides of the _really_ cloudy one. It sort of looked akin to rhizomorphic (I think I'm using the term correctly) growth but darkish and growing along the inside of the jar. I'm watching the other one now, as it doesn't look as bad. But it doesn't look great, either. I guess I'll just let it go and try using it to innoc a jar or two.
Any good links to check with karo pics/tips?
Thanks!


--------------------
Jerry Garcia. JERRY GARCIA! JERRY GARCIA!!!!


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Offlinescatmanrav
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Re: My f'ing Karo jars keep comin' up clouds [Re: GnuBobo]
    #3241589 - 10/11/04 03:07 AM (12 years, 1 month ago)

Well you can see an above one I made with some grains of rye.

Only way to know for sure is to try them though.


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


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InvisibleGnuBobo
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Re: My f'ing Karo jars keep comin' up clouds [Re: scatmanrav]
    #3241728 - 10/11/04 03:48 AM (12 years, 1 month ago)

Thanks.  I guess I'm trying to figure out when they're "ready."  Because I've had threads of myc in my jars before.  And they've been cloudy.  But you want it to have visible myc threads/clouds that are relatively substantial before you use for innoc, right? 
    That pic looks a lot like my jars.  I'm just not seeing much threading.  Is this kind of "cloudy" water good for innoculating jars? 
    I've just had some bad luck with karo, I guess.  And Bacillus.  That stuff SUCKS.  Thanks man!  :grin: :thumbup:

GB


--------------------
Jerry Garcia. JERRY GARCIA! JERRY GARCIA!!!!


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OfflineMadHatR
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Re: My f'ing Karo jars keep comin' up clouds [Re: GnuBobo]
    #3241912 - 10/11/04 05:22 AM (12 years, 1 month ago)

I to get the clouds with karo. Not sure if it the nature of karo, the brand I picked or what. Have used it before though and seems to work fine. Desided to switch back to malt though, seem to get better results and more clumping and threading. plus I can toss the karo in my wbs when simmering with water and coffee. Getting good results that way and still have a use for the karo.


--------------------
Following the rules is for dealers, knowing why the rules work is for innovators.

"Knowing is half the battle." - G.I. Joe

Never Judge a man until you walk a mile in his shoe, because then you are a mile away and you have his shoes.


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Offlinescatmanrav
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Re: My f'ing Karo jars keep comin' up clouds [Re: MadHatR]
    #3242488 - 10/11/04 12:32 PM (12 years, 1 month ago)

Its just mycelium. When you spin the water around (I spin it one way then the other back and forth a few times to rip it up) little peices of myc are everywhere. They just might not be stringy to your eye. I just wait until when I spin the karo jars I can see a cloud of small white things spinning around in the entire jar. Then while its still spinning tilt it quickly and suck up what you need. I then use 2-3cc's. I've found 3 to be good unless the substrate is already on the wet side, then 2.

Certain contams can look cloudy but its different, its not slightly cloudy, its a whitness tinge to the water. Kinda hard to explain. All of my Karo jars havn't been done in a glove box (all 10 of my strains) and I haven't had a problem yet, even using grain to inoculate except for one strain the first time I did it but I use old myc from like 3rd or 4th gen grain to grain transfers. You don't want to do that if you are. If you are either use some spores or a piece of a mushroom (inside stem with a little h202).


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


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Offlinejon_funk
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Re: My f'ing Karo jars keep comin' up clouds [Re: scatmanrav]
    #3242816 - 10/11/04 02:20 PM (12 years, 1 month ago)

I made some honey water, sprayed in some spores from a syringe i kept from months ago that i thought where inactive (was given the spores for free since they where old and probably wouldn't work)
They didn't work injected into a cake, but in this honey water...:

Sprayed out the syringe into the water, Forgot about for around 2 weeks,
I look back a few days ago and the bottom of the glass is filled with fluffy, marble sized balls of Mycellium! Not sure how i'm gonna be able to suck em into a syringe but we'll see!!!

:laugh:

Jonny


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Offlinescatmanrav
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Re: My f'ing Karo jars keep comin' up clouds [Re: jon_funk]
    #3243038 - 10/11/04 03:08 PM (12 years, 1 month ago)

Swirl the water around and suck in water into the syringe and squirt it at the chunks and repeat the process using the water to break it up.

Syringes can last for many years. I've heard much talk of people keeping them up to 2 years but I've heard of up to 10 years in one case and they still worked...


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


Post Extras: Print Post  Remind Me! Notify Moderator
OfflinePsilocybeingzz
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Re: My f'ing Karo jars keep comin' up clouds [Re: GnuBobo]
    #3243340 - 10/11/04 03:54 PM (12 years, 1 month ago)



My "clean" CYAN liquid culture.
:grin:
(is a good angle, and the backround is pretty cool to)
I wanted the pic to really capture the clear liquid, remember kids, dont use to much corn syrup(honey etc...use a ratio of 4cc's-Karo/94cc's-Water), and dont heat your jars to long :wink:


--------------------


Edited by Psilocybeingzz (10/11/04 04:01 PM)


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Offlinescatmanrav
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Re: My f'ing Karo jars keep comin' up clouds [Re: Psilocybeingzz]
    #3243404 - 10/11/04 04:12 PM (12 years, 1 month ago)

That is a great pic, the best one I got was above. You should swirl that though and get a pic of what it looks like all mixed up. This is where I think people just starting with liquid cultures have trouble determining it.


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


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InvisibleGnuBobo
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Re: My f'ing Karo jars keep comin' up clouds [Re: Psilocybeingzz]
    #3244221 - 10/11/04 07:36 PM (12 years, 1 month ago)

See, THAT is what I've been trying to get from karo. A little cloud. Not a London Fog everywhere. I swear to god it's the strain this time around. My EQ jars colonized far differently, e.g. evenly. The grain jars I have now with the hawaiians are doing this fucked up spotty colonization. The myc looks healthy where it is, it's just slow and strange. LIke someone stuffed cottonballs in my grain jars at random points.
Well, we'll see how the karo progresses. Maybe I'll post a pic of my jar tonight. What's the best way to light a karo jar for a pic? I've tried several ways and it never comes out very well. Thanks!

GB


--------------------
Jerry Garcia. JERRY GARCIA! JERRY GARCIA!!!!


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Offlinescatmanrav
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Re: My f'ing Karo jars keep comin' up clouds [Re: GnuBobo]
    #3244334 - 10/11/04 08:06 PM (12 years, 1 month ago)

Natural light with no flash seems to be best.


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


Post Extras: Print Post  Remind Me! Notify Moderator
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Amazon Shop for: ½ Pint Jars, Rye Grain, Wild Bird Seed

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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