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OfflineSingularity
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Adding L-Tryptophan to substrate
    #3235299 - 10/09/04 02:49 AM (12 years, 1 month ago)

I have extensively read posts on this forum and other regarding added tryptophan to substrate, but I have been unable to find anything more than just theory on this subject.

Has anybody had any personal experience with this, or know for a definate fact if this method will be effective?

Also, are there any other nutrients that can be added to substrate to potentiate the psilo content in mushrooms, and/or improve growth (becides vitamin B1)?

Thanks.


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InvisiblePrisoner#1M
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Re: Adding L-Tryptophan to substrate [Re: Singularity]
    #3235351 - 10/09/04 03:04 AM (12 years, 1 month ago)

Quote:

Singularity said:
but I have been unable to find anything more than just theory on this subject.





what have you deduced from your research on it... I've used tryptamine HCL
in a substrate, it gave an artificial feeling trip, it felt plastic, regardless
you should give it a shot, there realy isnt anything we have available that will allow us
to reliably measure any differences but you can give us a personal account as
to how it differed from previous trips


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Offlinediscman1
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Re: Adding L-Tryptophan to substrate [Re: Prisoner#1]
    #3235895 - 10/09/04 09:40 AM (12 years, 1 month ago)

How about you just throw some chopped up turkey into your substrate? :lol: :confused: :crazy2: :stoned: :wtf: :eyemouth: :jazzman: :what: :goodluck:

Edit: On a more serious note, I noticed your reference to Vitamin B1. I've been staring at my bottle of SuperThrive for a while, wondering if it had any potential benefits for shrooms, but I hadn't really run across anything. So what is the story with that?

It also contains a plant rooting hormone(1-Napthyl acetic acid), which I doubt has any affect on mushrooms, but what do I know..?


Edited by discman1 (10/09/04 09:44 AM)


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Offlineimjrod
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Re: Adding L-Tryptophan to substrate [Re: discman1]
    #20786788 - 11/03/14 04:28 AM (2 years, 1 month ago)

I've been feeding D-L tryptophan to my shroom cultures for many years. To my experience, I don't have to eat as many caps in comparison to those not fed tryptophan. Potency generally translates into eating less shrooms versus a better trip.

Vitamin B1 makes the shrooms have a better consistency, and "look healthier" but I wouldn't say it increases yield. I feed shrooms D-L tryptophan and vitamin B1 as per standard procedure.

Feeding Ergot D-L tyrptophan and vitamin B is also common practice when culturing Ergot to make acid. Shrooms should be treated pretty much the same way. Use D-L tryptophan as a nutrient in the substrate and in the water between flushes. How much? I always use the "too much won't hurt theory." I don't really know how much precisely but what I do is dissolve about as much as the water can handle without supersaturating. I'm sure this is probably more than the shrooms can use but it doesn't hurt them.

I wouldn't use tryptophan HCL, and not 5 HTP, either. Just D-L tryptophan. So, yes, adding D-L tryptophan to your substrate will most likely make it to where you don't have to eat as many of them shrooms. Psilocybin is mostly made during fruit cycle so that would be the crucial time to use it. However I use it in grain spawns and LCs as well as rice/verm.

It seems to makes a bigger difference on slow growers. For example, Puerto Rico grows real fast up until it starts pinning, then the shrooms slow down. The Tryptophan makes a bigger difference during that slow period. In Orissa, the caps take a while to grow. In my experience, when the shrooms grow slower or have a "slow period," are affected much more noticeably by adding Tryptophan than others are.  The main if not only difference is that we don't have to eat as much.


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Offlinespore baby
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Re: Adding L-Tryptophan to substrate [Re: imjrod]
    #20786796 - 11/03/14 04:41 AM (2 years, 1 month ago)

Quote:

imjrod said:
I've been feeding D-L tryptophan to my shroom cultures for many years. To my experience, I don't have to eat as many caps in comparison to those not fed tryptophan. Potency generally translates into eating less shrooms versus a better trip.

Vitamin B1 makes the shrooms have a better consistency, and "look healthier" but I wouldn't say it increases yield. I feed shrooms D-L tryptophan and vitamin B1 as per standard procedure.

Feeding Ergot D-L tyrptophan and vitamin B is also common practice when culturing Ergot to make acid. Shrooms should be treated pretty much the same way. Use D-L tryptophan as a nutrient in the substrate and in the water between flushes. How much? I always use the "too much won't hurt theory." I don't really know how much precisely but what I do is dissolve about as much as the water can handle without supersaturating. I'm sure this is probably more than the shrooms can use but it doesn't hurt them.

I wouldn't use tryptophan HCL, and not 5 HTP, either. Just D-L tryptophan. So, yes, adding D-L tryptophan to your substrate will most likely make it to where you don't have to eat as many of them shrooms. Psilocybin is mostly made during fruit cycle so that would be the crucial time to use it. However I use it in grain spawns and LCs as well as rice/verm.

It seems to makes a bigger difference on slow growers. For example, Puerto Rico grows real fast up until it starts pinning, then the shrooms slow down. The Tryptophan makes a bigger difference during that slow period. In Orissa, the caps take a while to grow. In my experience, when the shrooms grow slower or have a "slow period," are affected much more noticeably by adding Tryptophan than others are.  The main if not only difference is that we don't have to eat as much.




Did you check how old this thread is before you posted?  :omgawesome:

Some of these people may be dead by now.  :wink:

:awecid:


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Offlineimjrod
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Re: Adding L-Tryptophan to substrate [Re: spore baby]
    #20786806 - 11/03/14 04:50 AM (2 years, 1 month ago)

No I guess I didn't see how old the thread was. But, however old the thread is, my question is, do you want stronger shrooms, or don't you?


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Offlinespore baby
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Re: Adding L-Tryptophan to substrate [Re: imjrod]
    #20786814 - 11/03/14 04:58 AM (2 years, 1 month ago)

I grew the strongest in the World.

P. azurescens and they are mighty strong enough. :awesomenod:


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Offlineimjrod
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Re: Adding L-Tryptophan to substrate [Re: spore baby]
    #20786839 - 11/03/14 05:22 AM (2 years, 1 month ago)

those aren't actually the strongest in the world but that's off topic


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Offlinespore baby
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Re: Adding L-Tryptophan to substrate [Re: imjrod]
    #20786842 - 11/03/14 05:24 AM (2 years, 1 month ago)

Yes they are.

Look under Effects here.

http://en.wikipedia.org/wiki/Psilocybe_azurescens

According to Paul Stamets, author of Psilocybin Mushrooms of the World,
the Psilocybe azurescens is the most potent of the tryptamine-bearing
mushrooms, containing up to 1.8% psilocybin, 0.5% psilocin, and
0.4% baeocystin by dry weight, averaging to about 1.1% psilocybin
and 0.15% psilocin.

Show me a stronger type if you can with proof.

:goodluck:


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Offlineimjrod
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Re: Adding L-Tryptophan to substrate [Re: spore baby]
    #20787858 - 11/03/14 02:14 PM (2 years, 1 month ago)

Well it's off topic, so no I won't go into it on this thread.


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Invisiblebodhisatta
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Re: Adding L-Tryptophan to substrate [Re: imjrod]
    #20787880 - 11/03/14 02:18 PM (2 years, 1 month ago)

imjrod,

Show us the proof.

Tryptophan get's broken down by the mycelium and then re-built. The mycelium cannot even uptake Tryptophan as is to use it as a precursor. Azures are one of the most potent psilocin/psilocybin containing species. Your posts are real shit.


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InvisibleStygianKnight
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Registered: 03/12/12
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Re: Adding L-Tryptophan to substrate [Re: imjrod]
    #20787883 - 11/03/14 02:19 PM (2 years, 1 month ago)

So on topic, are you bothering to isolate?
Do you realize strain names mean nothing?


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InvisibleMr. Alien
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Re: Adding L-Tryptophan to substrate [Re: spore baby]
    #20787940 - 11/03/14 02:32 PM (2 years, 1 month ago)

Quote:

spore baby said:
Show me a stronger type if you can with proof.





My PE. Just kidding.


--------------------

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Offlinespore baby
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Re: Adding L-Tryptophan to substrate [Re: bodhisatta]
    #20787943 - 11/03/14 02:33 PM (2 years, 1 month ago)

Quote:

bodhisatta said:
imjrod,

Show us the proof.

Tryptophan get's broken down by the mycelium and then re-built. The mycelium cannot even uptake Tryptophan as is to use it as a precursor. Azures are one of the most potent psilocin/psilocybin containing species. Your posts are real shit.




Actually you have to feed tryptamine into it.

Use the damn Shroomery search engine next time duh.  :rolleyes:

https://www.shroomery.org/9040/Tryptamine-Cubensis


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Offlineimjrod
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Re: Adding L-Tryptophan to substrate [Re: bodhisatta]
    #20787966 - 11/03/14 02:41 PM (2 years, 1 month ago)



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Offlineimjrod
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Re: Adding L-Tryptophan to substrate [Re: imjrod]
    #20787979 - 11/03/14 02:45 PM (2 years, 1 month ago)

Pan Cams are the strongest. They are annuals. Ya plant them outside and they keep coming back every year. I planted mine several years ago and pick them every summer. I don't give them tryptophan though LOL What's funny is giving the stuff we grow precursors is a standard procedure and there are places here on shroomery documenting a 3 times increase in potency by feeding shrooms the precursor. I love the irony, that elsewhere on the site is the proof trolls are harassing me about.


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InvisibleMunchauzen
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Re: Adding L-Tryptophan to substrate [Re: imjrod]
    #20788007 - 11/03/14 02:50 PM (2 years, 1 month ago)

Quote:

imjrod said:
They are annuals. Ya plant them outside and they keep coming back every year.




you men perennial. trust me, I'm a gardener. I ripped out probably 1000 pounds of annuals last week.


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Offlineomegafaust
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Re: Adding L-Tryptophan to substrate [Re: imjrod]
    #20788010 - 11/03/14 02:51 PM (2 years, 1 month ago)

Where is the proof?!

If it's true, awesome, but we like to see proof here.

And this is the first time I have heard a mushrooms species referred to as annual x)


--------------------
The Universe has an interesting sense of irony, in that you are the universe experiencing itself.  All you are is a thought.


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Offlineomegafaust
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Re: Adding L-Tryptophan to substrate [Re: omegafaust]
    #20788016 - 11/03/14 02:52 PM (2 years, 1 month ago)

No he meant annuals. 

Earlier he claimed mold was not a fungus.


--------------------
The Universe has an interesting sense of irony, in that you are the universe experiencing itself.  All you are is a thought.


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InvisiblePestile

Registered: 05/02/13
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Loc: Northern Europe
Re: Adding L-Tryptophan to substrate [Re: imjrod]
    #20788022 - 11/03/14 02:54 PM (2 years, 1 month ago)

Quote:

imjrod said:
Pan Cams are the strongest.




Source, please?


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Open Source Intelligence News



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