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Offlinealisia
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Registered: 09/01/04
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Last seen: 12 years, 5 months
mushrooms clippings instead of innoculating cakes?
    #3233965 - 10/08/04 06:59 PM (12 years, 7 months ago)

it work well dropping a small mycelium clipping from a colonized cake into a sterilized 1 pint jar of prepared substrate? wil this colonize well or is there to much chance for contams?


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InvisibleDiploidM
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Registered: 01/09/03
Posts: 19,274
Loc: Rabbit Hole
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Re: mushrooms clippings instead of innoculating cakes? [Re: alisia]
    #3233978 - 10/08/04 07:06 PM (12 years, 7 months ago)

It will work, but the jar has to remain sterile after the transfer or the contamination introduced will win the race to colonies the new substrate.

That means the piece from the colonized cake has to be sterile, the thing you use to cut it must be sterile, and the air around the jar must be sterile while the jar is open. This is hard to do without a flowhood and some other tools, but it is possible.

Good luck!  :mushroom2:


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Offlinecharvo
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Registered: 03/09/03
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Re: mushrooms clippings instead of innoculating cakes? [Re: Diploid]
    #3234499 - 10/08/04 10:11 PM (12 years, 7 months ago)

kinda like G2G or wedge 2G


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OfflineHambo
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Registered: 09/23/04
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Re: mushrooms clippings instead of innoculating cakes? [Re: charvo]
    #3234621 - 10/08/04 10:55 PM (12 years, 7 months ago)

sterilise tools, use oven tek.. and dunk bit in h202?


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OfflineCryogeniczV
what?
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Re: mushrooms clippings instead of innoculating cakes? [Re: Hambo]
    #3234682 - 10/08/04 11:14 PM (12 years, 7 months ago)

sounds like fun.


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