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 oOTerraSanOo
journeyman
Registered: 04/30/01
Posts: 57
Last seen: 17 years, 6 months
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 help with innoculation... 
#323284 - 05/22/01 12:11 PM (17 years, 7 months ago) |
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AFOAF has about 6 cups of liquid mycelium medium ready to go. She plans to innoculate quart jars of whole millet mixed 2 to 1 with distilled water and pressure cooked @15pds for 1 hour. Should she put h2o2 in the liquid medium before transferring and if so how much for each 2 cups? Also how many cc's of medium should she put in each jar of millet? She put 1 1/2 cups of millet and 3/4 cup water in each jar before pressure cooking. Which fills each jar about 2/3rds of the way.
Also, she plans on innoculating through the filter and aluminum foil on top then taping the hole, is this a good idea?
Thanks for any help!
--
"The work will teach you how to do it."
--Estonian proverb
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 puscle
genius of love
Registered: 01/06/01
Posts: 4,539
Loc: NY
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 Re: help with innoculation... [Re: oOTerraSanOo]
#323322 - 05/22/01 12:46 PM (17 years, 7 months ago) |
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1 - 2 cc`s of peroxide per 1 cup liquid. Yes, the peroxide goes in first. I`d inoculate with 5 - 10 cc`s per jar. Her inoculation method sounds fine.
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oOTerraSanOo
journeyman
Registered: 04/30/01
Posts: 57
Last seen: 17 years, 6 months
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Re: help with innoculation... [Re: puscle]
#323377 - 05/22/01 01:50 PM (17 years, 7 months ago) |
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OK, I hope my friend didn't screw this up. But she just looked at the syringe and the measurments are in ml's, she put two ml's of h2o2 in each 2 cups of liquid medium, did she just kill it all?
--
"The work will teach you how to do it."
--Estonian proverb
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Billyblastoff
Psychoactivepredator
Registered: 04/20/01
Posts: 725
Loc: Down these mean city stre...
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Re: help with innoculation... [Re: oOTerraSanOo]
#323806 - 05/22/01 10:36 PM (17 years, 7 months ago) |
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ml's and cc's are the same as far as units of measure go.
Tell me something good then I might agree with you..
--------------------
"Imagination is more important than knowledge. For while knowledge defines all we currently know and understand, imagination points to all we might yet discover and create."
"There is a wealth of information built into us, tucked away in the genetic material in every one of our cells. The psychedelic drugs allow exploration of this interior world, and insights into its nature."
Tell me something good then I might agree with you....the Smithereens
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