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Amazon Shop: ½ Pint Jars, Agar, Laminar Flow Hood

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Registered: 10/04/04
Posts: 27
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Agar to substrate xfer
    #3232452 - 10/08/04 11:37 AM (13 years, 8 months ago)

Hi guys, jus curious how to transfer a culture in agar-agar to a fruiting medium or case.. coulnd?t find it.

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Brainy Smurf

Registered: 05/08/04
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Re: Agar to substrate xfer [Re: cucumelero]
    #3233530 - 10/08/04 05:08 PM (13 years, 8 months ago)

Take a sterilized scalpel (or knife of some sort) and cut a wedge of the agar plate off and put it in the substrate!

"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay

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Re: Agar to substrate xfer [Re: scatmanrav]
    #3234737 - 10/08/04 11:30 PM (13 years, 8 months ago)

Wash hands with antibacterial soap. Turn on Flow hood (let run for about 5 mins) or sterilize glove box or use oven tek. Clean hands with alcohol by pouring a small amount to cover your hands and rub them together in front of flow hood. Sterilize scalpel by dipping in rubbing alcohol, and then set on fire, then wipe and cool with alcohol soaked cotton ball. Then use sterile blade to cut 2 wedges and insert both into grain jar, close lid, and incubate.

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Edited by Cryogenicz (10/08/04 11:31 PM)

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born to grow
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Re: Agar to substrate xfer [Re: Cryogenicz]
    #3235059 - 10/09/04 01:08 AM (13 years, 8 months ago)

I use Purell hand disinfectant :wink: it works much better than straight alcohol :wink:

Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"

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Amazon Shop: ½ Pint Jars, Agar, Laminar Flow Hood

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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