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Offlinecucumelero
Mr.
Registered: 10/04/04
Posts: 27
Last seen: 12 years, 8 months
Print directly to water
    #3232232 - 10/08/04 10:39 AM (12 years, 10 months ago)

Hi Guys,
just wondering if you can make prints directly to water to fill the siringes just right there.  The idea will be to have a beaker and the shrom on top then using the siringe right there, any experience? the idea will be to use them right away... :rolleyes:

Another question, can make prints from dried shrooms? I know that they will have significanlly less spores, but they should have at least, right?
Thanks
:rocket:


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OfflinekronnyQ
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Registered: 07/23/04
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Re: Print directly to water [Re: cucumelero]
    #3232370 - 10/08/04 11:20 AM (12 years, 10 months ago)

Yes, this is a very good way to do it, it's what I do. There is a tek on how to do it somewhere on here, but if you can't find it i will just explain how I do it:

Take a sterilized shot glass, fill it with 10ccs of distilled water, just enough for a syringe. You need to do this in a dust free area as well if you want to keep it clean.

Use some sterilized wires to make an 'X' over top the shot glass. Take a fresh cap and set on top of the wire support. Cover the shot glass with a sterilized jar and wait. I usually give it 8-16 hours or so, the longer you wait the more spores.

After waiting get a sterile syringe, and slowly remove jar that is overtop shot glass. I use a pin to prick the cap and remove it. Tip the shot glass a little bit sideways and draw out the liquid with your syringe. Boom there it is.

This method is good but hard to keep contams out without a floodhood or glovebox, i do it infront of a hepa in my lab and it seems to work fine.

You can get a few spores from dried shrooms, but not many...it's really just better to use fresh.


Edited by kronnyQ (10/10/04 11:50 AM)


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OfflineAnnoA
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Re: Print directly to water [Re: kronnyQ]
    #3232396 - 10/08/04 11:24 AM (12 years, 10 months ago)

>There is a tek on how to do it somewhere on here

http://www.shroomery.org/index/par/7923/pag/5


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Offlinecucumelero
Mr.
Registered: 10/04/04
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Re: Print directly to water [Re: Anno]
    #3232430 - 10/08/04 11:30 AM (12 years, 10 months ago)

thanks to both


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Offlinescatmanrav
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Registered: 05/08/04
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Trusted Cultivator
Re: Print directly to water [Re: kronnyQ]
    #3233516 - 10/08/04 05:05 PM (12 years, 10 months ago)

Quote:

kronnyQ said:
Yes, this is a very good way to do it, it's what I do. There is a tek on how to do it somewhere on here, but if you can't find it i will just explain how I do it:

Take a sterilized shot glass, fill it with 10ccs of distilled water, just enough for a syringe. Add half a capful of peroxide to it. You need to do this in a dust free area as well if you want to keep it clean.




This is what you do? Have you actually tried any of your syringes yet. If you add peroxide to your spore syringes the WONT work. Sorry.


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


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OfflineLeukoism
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Registered: 09/18/04
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Re: Print directly to water [Re: scatmanrav]
    #3233650 - 10/08/04 05:30 PM (12 years, 10 months ago)

Ya, thats true the peroxide will kill the spores.


--------------------
May it have been the one that you are no longer aware, but now it has become a reality.

-----Enter the craze untill you have seen!-----

--------------------------


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OfflinekronnyQ
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Re: Print directly to water [Re: Leukoism]
    #3238784 - 10/10/04 11:49 AM (12 years, 10 months ago)

Shit...peroxide doesn't kill mycellium so I thought it would be fine for spores... I only use a small amount, I really don't think it kills many spores cuz I grew some mycellium in honey water that had a little H2O2 in it. I'm getting some malt powder real soon here so I will inject some of my syringes in some solution and see if they are dead.

Oh well my method still works good just don't use H2O2


Edited by kronnyQ (10/10/04 11:50 AM)


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