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Offlineeazygrowin
Stranger
Registered: 09/14/04
Posts: 11
Last seen: 11 years, 9 months
New syringe ?uestion
    #3230901 - 10/07/04 11:57 PM (12 years, 9 months ago)

HI, Well Tonight I just got done pressure cooking 3 batches of 3 pint WBS jars in the new 4.2q $17 dollar cooker. Right now they are cooling in the oven, and tommorow I plan on Innoculating them. My question comes in making a syringe. I purchased 2, 22guage sterile packaged syringes. Do I still need to boil water in a suace pan and use it to sterilize the syringe, or is this for sterilization of the water? Also is it necessary to let the syringe sit for the period after boiling the water before I can proceed to scraping the spore print into a shot glass and then sucking it up? How long should I let the new spore syringe sit before I inoculate? I would like to inoculate the jars within 12 hours. However I do want to do it right. So any advice or imput would be greatly appriciated.
Thank you,
Eazy Growin


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Offlineatomic1
enthusiast
Male

Registered: 09/18/03
Posts: 1,105
Loc: Appalachia
Last seen: 4 months, 4 days
Re: New syringe ?uestion [Re: eazygrowin]
    #3231470 - 10/08/04 01:36 AM (12 years, 9 months ago)

Check this out:
http://www.shroomery.org/index/par/22995

Yeah, boiling your water would be for the water...the syringe will be sterile.  Just let it sit long enough to cool down. :thumbup:


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InvisibleOldSpice
Geritol Breath...
Female User Gallery

Registered: 08/25/03
Posts: 59,080
Loc: Crankytown, Texas
Re: New syringe ?uestion [Re: eazygrowin]
    #3231514 - 10/08/04 01:54 AM (12 years, 9 months ago)

You dont have to wait for the spores to hydrate just shake the shit out of the syringe before innoculating


--------------------
So hard to be ....WDWGFH?
Texas is humongus compared to France
Our Gair, who art in Texas,
Paw Paw be thy Name....
My friends are thirsty


You never see a motorcycle parked outside a Psychiatrist office:biker:


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InvisibleSam1912
journeyman

Registered: 09/23/04
Posts: 2,142
Loc: Cali
Re: New syringe ?uestion [Re: atomic1]
    #3231527 - 10/08/04 02:03 AM (12 years, 9 months ago)

I just went through this. I had sterile water and containers, though, so a bit differnt. You'll need a source of sterile water. It's prudent to follow the whole procedure unless you have them because you'll need to suck in and out on water few times to break up the spores chunks. Contamination at this stage will ruine all your crops. So, it's better, IMHO, to be prudent.

I got response from 12 hours to 2 days for rehydrating spores in the syrange. I had mine in the water for 20 hours and it worked out great. After you make the syringes, put them in a zip lock bag(keep it as sterile as possible) without the needles at room temperature. I've read that you can inoculate it immediately but it'll slow down the colonization dramatically.

I hope that helps.

Sam


--------------------
Protect your civil rights!  End drug prohibition.  And if you don't care about your civil rights, protect mine!

If you want a rating from me, please PM me.  For those really newbies, don't expect an answer back, but you can try me anyways.


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InvisibleOldSpice
Geritol Breath...
Female User Gallery

Registered: 08/25/03
Posts: 59,080
Loc: Crankytown, Texas
Re: New syringe ?uestion [Re: Sam1912]
    #3231553 - 10/08/04 02:15 AM (12 years, 9 months ago)

Waiting for spores to hydrate doesnt slow them down that much....
Shurely doesnt slow anything down dramatically


--------------------
So hard to be ....WDWGFH?
Texas is humongus compared to France
Our Gair, who art in Texas,
Paw Paw be thy Name....
My friends are thirsty


You never see a motorcycle parked outside a Psychiatrist office:biker:


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InvisibleSam1912
journeyman

Registered: 09/23/04
Posts: 2,142
Loc: Cali
Re: New syringe ?uestion [Re: OldSpice]
    #3231618 - 10/08/04 02:44 AM (12 years, 9 months ago)

Actually, PF Tek here says it's very important you let them rehydrate completely before inoculation. But some of the other derivatives says to go ahead and inoculate if you are in a hurry.

Sam


--------------------
Protect your civil rights!  End drug prohibition.  And if you don't care about your civil rights, protect mine!

If you want a rating from me, please PM me.  For those really newbies, don't expect an answer back, but you can try me anyways.


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InvisibleOldSpice
Geritol Breath...
Female User Gallery

Registered: 08/25/03
Posts: 59,080
Loc: Crankytown, Texas
Re: New syringe ?uestion [Re: Sam1912]
    #3231635 - 10/08/04 02:50 AM (12 years, 9 months ago)

Im not in that much of a hurry...I wish they would colonise slower *smiles
xxxx`s n oooo`s my friend


--------------------
So hard to be ....WDWGFH?
Texas is humongus compared to France
Our Gair, who art in Texas,
Paw Paw be thy Name....
My friends are thirsty


You never see a motorcycle parked outside a Psychiatrist office:biker:


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Offlinescatmanrav
Brainy Smurf

Registered: 05/08/04
Posts: 11,483
Loc: Flag
Last seen: 4 years, 6 months
Trusted Cultivator
Re: New syringe ?uestion [Re: OldSpice]
    #3232845 - 10/08/04 01:34 PM (12 years, 9 months ago)

Tell your friend rav said shes yummy :smile:

Shrooms will rehydrate on the substrate too, you added water right?


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


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