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Offlinepetepetosa
newbie
Registered: 12/06/00
Posts: 19
Loc: West USA
Last seen: 20 years, 8 months
Honeywater/Birdseed
    #323046 - 05/22/01 10:42 AM (21 years, 4 months ago)

Mixed 1 pint dry birdseed with 1/2 pint honeywater in 3 quart jars and just plain spring water in 3 others. Then pressure cooked for one hour. Let sit for a week, no contams evident. Inoculated with syringes 3 nights ago, putting 5cc in each. B+, Cambodian, and Thai. Using an extremely sterile room with hepa filter running for a week before starting. Sprayed with bleach water and alcohol everyday. All jars were put into sterlite container that had been wipied out with bleach, then alcohol, then sprayed down with Hydrogen Peroxide. Thermostat set at 75 degrees (which is probably too cool).

No contams showing so far, but no sign of anything else either. Just damp birdseed. Could I have oversterlized and killed my spores? The syringes were in a plastic bag with a little alcohol in it. I wiped them down and flame sterilized the needle before each jar.

I was trying to detect (and hoping for) if the honeywater mix would grow faster than springwater. Should I raise the temp? I was hoping for a sign of something by now... I have a history of being impatient of such things I guess, but could use some input on the whole thing....Anyone ever tried this???

pete



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Say what you mean
Mean what you say


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OfflineSeussA
Error: divide byzero

Folding@home Statistics
Registered: 04/27/01
Posts: 23,480
Loc: Caribbean
Last seen: 8 months, 2 days
Re: Honeywater/Birdseed [Re: petepetosa]
    #325182 - 05/25/01 12:05 AM (21 years, 4 months ago)

Be patient. It usually takes my friend a four days before
she starts to notice growth in the jars. She claims that
the first signs of growth in the jars looks almost like a
light film between the glass and the substrate. After a day
the film turns fuzzy white and takes off.

Your temperature should be fine. Cooler temps slow down
the growth a little bit, but the cooler temps usually slow
down the growth of contaims even more giving the mycelium
a better change of winning any battles.

There _might_ be too much water in your mix. My friend
said she had never used birdseed and didn't know if your
proportions were correct.

Give it a good week before you start to worry too much.



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Just another spore in the wind.


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Offlinedioze1
Satans minion inthe war onlaughter
Registered: 11/24/00
Posts: 384
Loc: Up a hogs ass looking for...
Last seen: 16 years, 9 months
Re: Honeywater/Birdseed [Re: petepetosa]
    #325474 - 05/25/01 04:44 AM (21 years, 4 months ago)

Patience is THE key. Every thing sounds Irie, so far. I agree, give it a "good" week--7-9 days, then get concerned.
Dioze1



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Not all that wander are lost.


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Offlinejonnyshaggs420
Pooh-Bah

Registered: 08/09/00
Posts: 1,965
Loc: Mid-West
Last seen: 17 years, 2 days
Re: Honeywater/Birdseed [Re: petepetosa]
    #325647 - 05/25/01 07:31 AM (21 years, 4 months ago)

Its probably just a little slow because of temps.  If it were contamination beleive me you would know by now.
My grandma once mixed a few jars with honey water instead of just water and, well she got a little preoccupied and forgot to sterilize for the proper amount of time. 3 days later the jars were completely colonized by cob web mold and the dreaded green mold.

A lesson to us all.  Sterilize for the full hour.

I can see a world where this is no poverty and no war, I can also see us attacking that world because they would never expect it. :wink:


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Vote Jonnyshaggs in the next election for GOD...Its the responsible choice


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Offlinepetepetosa
newbie
Registered: 12/06/00
Posts: 19
Loc: West USA
Last seen: 20 years, 8 months
Re: Honeywater/Birdseed [Re: petepetosa]
    #326154 - 05/26/01 05:57 PM (21 years, 4 months ago)

Thanks for the support......

I am waiting....

Say what you mean
Mean what you say


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Say what you mean
Mean what you say


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Offlineshroomeatindude
journeyman
Registered: 05/05/01
Posts: 79
Last seen: 21 years, 29 days
Re: Honeywater/Birdseed [Re: petepetosa]
    #326239 - 05/26/01 08:48 PM (21 years, 4 months ago)

Too wet substrate wont colonize. im not sure about your porportions though, they may be fine. Next time steep your seed first, then you dont need to worry abput this. Friend says 10 degrees higher would be optimum though!


-Shr00meatind00d


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-Shr00meatind00d


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Offlinepetepetosa
newbie
Registered: 12/06/00
Posts: 19
Loc: West USA
Last seen: 20 years, 8 months
Re: Honeywater/Birdseed [Re: petepetosa]
    #338913 - 06/12/01 01:48 PM (21 years, 3 months ago)

One jar started a little colonization and stopped. The rest of them did absolutely nothing. 5 syringes down the drain.

Say what you mean
Mean what you say


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Say what you mean
Mean what you say


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OfflineMNmyc
enthusiast

Registered: 02/26/01
Posts: 175
Loc: MN
Last seen: 15 years, 6 months
Re: Honeywater/Birdseed [Re: petepetosa]
    #339718 - 06/13/01 12:40 PM (21 years, 3 months ago)

5?! SYRINGES?!!!!!! I finally did my first honey water thing just today. I geuss yesterday. I don't feel so good now. I was afraid that the myc. wouldn't like the honey, because of it's anti-microbial properties. I just figured that it must be so diluted as to be rendered ineffective in that regard. Like when I make honey wheat bread.

Is there any forensic evidence that you could interprete to be an unrelated (to honey) cause of failure? Like moisture content as it relates to substrate?

This excerpt and all characters contained within are entirely fictional...
Support the FSR and micronmagick


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Life is what happens while you're making other plans...


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