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Invisiblenotsloth
Stranger

Registered: 09/10/04
Posts: 50
stem + h2o2 into karo ?
    #3221224 - 10/06/04 01:51 AM (12 years, 2 months ago)

My apologies if this is a stupid question, but if it would work it would make my life a lot easier :smile:

Would it be possible to cut the stem of a mushroom into tiny pieces, soak it in Hydrogen Peroxide and then inject it into a Karo jar? Could I expect to grow myc, mold, or nothing at all ? Would this be a way to clone without a glovebox in a non-sterile environment? I'm hoping that the peroxide would kill off the contams, and the tissue itself (protected by enzymes, or possibly voodoo) would survive to colonise the jar?

Thanks


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Offlinescatmanrav
Brainy Smurf

Registered: 05/08/04
Posts: 11,483
Loc: Flag
Last seen: 3 years, 10 months
Trusted Cultivator
Re: stem + h2o2 into karo ? [Re: notsloth]
    #3221379 - 10/06/04 02:25 AM (12 years, 2 months ago)

It is quite possible! You may get contams but try a few and I bet at least one would be viable. Good luck.


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


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InvisibleCitricM
 User Gallery

Registered: 03/19/04
Posts: 4,207
Trusted Cultivator
Re: stem + h2o2 into karo ? [Re: notsloth]
    #3221400 - 10/06/04 02:35 AM (12 years, 2 months ago)

http://www.shroomery.org/index/par/9

http://www.shroomery.org/index/par/23544


You cannot do this in open air. You need a glovebox / flow hood.


--------------------
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Magash:  I noticed my contams were in the shape of fingers :whack:
Hyphae:  Yes  "Loss of moisture from the substrate"  is not a casing trigger.  :cuckoo:

My final Grow!


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Offlinemrjim202000
barefoot andfree
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Registered: 02/20/04
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Re: stem + h2o2 into karo ? [Re: notsloth]
    #3221820 - 10/06/04 09:27 AM (12 years, 2 months ago)

if you use steril (sp?) blades to cut and a needle to handle the peices then you "can" have very good results if you take clean center cuts from the inside of the stem or cap
my 2 cents
good luck
peace


--------------------
dont tell lies, there will be less to remember


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Offlinescatmanrav
Brainy Smurf

Registered: 05/08/04
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Last seen: 3 years, 10 months
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Re: stem + h2o2 into karo ? [Re: notsloth]
    #3222167 - 10/06/04 12:35 PM (12 years, 2 months ago)

You also CAN do this in open air if you are sterile. Especially adding h202.


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


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Invisiblenotsloth
Stranger

Registered: 09/10/04
Posts: 50
Re: stem + h2o2 into karo ? [Re: notsloth]
    #3222920 - 10/06/04 03:26 PM (12 years, 2 months ago)

Thats the answer I was hoping for :smile:

So if I understand correctly, while h2o2 is not a substitute for cleanliness and sterile procedures, its a worthwhile addition and greatly increases my chances if I want to clone without a glovebox.

Thank you again, fellow shroomerites, for your invaluable assistance in this endeavour  :heart:


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Offlinediscman1
journeyman
Registered: 08/24/04
Posts: 962
Last seen: 11 years, 11 months
Re: stem + h2o2 into karo ? [Re: notsloth]
    #3222964 - 10/06/04 03:37 PM (12 years, 2 months ago)

What if you sterilized a syringe, and used the needle to "scoop" some internal mushroom tissue into the bore of the needle? You would end up with a small plug of tissue that should be sterile assuming the inside of the needle was sterile.

You would still have to worry about the outside of the needle having contams on it, but that is better and more easy to deal with than the tissue itself being exposed to air and becomming contaminated.

Just a newb idea I had while reading this thread. :smile:


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Offlinediscman1
journeyman
Registered: 08/24/04
Posts: 962
Last seen: 11 years, 11 months
Re: stem + h2o2 into karo ? [Re: discman1]
    #3225928 - 10/07/04 02:54 AM (12 years, 2 months ago)

Nobody has any comment on my idea?

I thought it was pretty spiffy myself, but maybe not. :P


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OfflineMadHatR
journeyman
Registered: 10/13/03
Posts: 253
Last seen: 8 years, 5 months
Re: stem + h2o2 into karo ? [Re: notsloth]
    #3226187 - 10/07/04 04:19 AM (12 years, 2 months ago)

You could build a ghetto glovebox out of a carboard box, plastic wrap for a window, and a trash bag pushed through and taped to 2 holes cut in the box. Lysol like crazy on the inside before and after putting your samples in, let it settle and do your work. It's not the best, but it is pretty much free.


--------------------
Following the rules is for dealers, knowing why the rules work is for innovators.

"Knowing is half the battle." - G.I. Joe

Never Judge a man until you walk a mile in his shoe, because then you are a mile away and you have his shoes.


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