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OfflineLeukoism
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Registered: 09/18/04
Posts: 272
Last seen: 11 years, 6 months
Liquid mycelium tek, help!!
    #3217049 - 10/05/04 03:56 AM (12 years, 2 months ago)

Ok, I want to try to grow some mycelium in some water, but the only tek I could find doesnt tell me how I can get spores from a mushroom into a syringe. I know its probably somwhere but I figure that everybody here could help. Also cant I use honey instead or malt? Please any teks and help is much needed!!


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May it have been the one that you are no longer aware, but now it has become a reality.

-----Enter the craze untill you have seen!-----

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Offlineangryjslice
now with 20%more anger
Male

Folding@home Statistics
Registered: 10/29/03
Posts: 916
Last seen: 8 months, 13 days
Re: Liquid mycelium tek, help!! [Re: Leukoism]
    #3217069 - 10/05/04 04:07 AM (12 years, 2 months ago)

make a spore print...then make a spore syringe.  many good teks here
http://www.shroomery.org/index/par/22995

then...microwave that shite with some karo like i do :wink:

http://www.shroomery.org/forums/showflat...7f0547201392946

~JSlice~


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Offlinescatmanrav
Brainy Smurf

Registered: 05/08/04
Posts: 11,483
Loc: Flag
Last seen: 3 years, 10 months
Trusted Cultivator
Re: Liquid mycelium tek, help!! [Re: Leukoism]
    #3217874 - 10/05/04 01:18 PM (12 years, 2 months ago)

See the FAQs for how to make a print and syringe then PC some (yes honey will work) honey water (1 tbsp per 100ml) for 15 minutes at 15 PSI. I've heard the microwave works too but I don't know for how long and if you have a PC you may as well just do it that way and be sure to get it right. Don't PC it for anymore or you'll carmilize it. Not even a minute more. Honey seemed to carmilize quicker then karo. Honey will give you a golden color making it harder to spot contams then karo but it should be light. If it gets darker you carmilized it. It will still work carmilized though IME (only tried twice worked both times after PCing for over an hour).


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"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


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InvisibleEvolving
Resident Cynic

Registered: 10/01/02
Posts: 5,385
Loc: Apt #6, The Village
Re: Liquid mycelium tek, help!! [Re: scatmanrav]
    #3217952 - 10/05/04 01:49 PM (12 years, 2 months ago)

I microwave for 10 minutes after boiling starts. Be careful though, it can boil over really easy in the microwave, leaving a sticky mess... you need plenty of extra space in the container.


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To call humans 'rational beings' does injustice to the term, 'rational.'  Humans are capable of rational thought, but it is not their essence.  Humans are animals, beasts with complex brains.  Humans, more often than not, utilize their cerebrum to rationalize what their primal instincts, their preconceived notions, and their emotional desires have presented as goals - humans are rationalizing beings.


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Offlinetheriddler
Stranger
Registered: 08/04/04
Posts: 18
Last seen: 11 years, 7 months
Re: Liquid mycelium tek, help!! [Re: scatmanrav]
    #3217988 - 10/05/04 01:58 PM (12 years, 2 months ago)

Quote:

scatmanrav said:
See the FAQs for how to make a print and syringe then PC some (yes honey will work) honey water (1 tbsp per 100ml) for 15 minutes at 15 PSI. I've heard the microwave works too but I don't know for how long and if you have a PC you may as well just do it that way and be sure to get it right. Don't PC it for anymore or you'll carmilize it. Not even a minute more. Honey seemed to carmilize quicker then karo. Honey will give you a golden color making it harder to spot contams then karo but it should be light. If it gets darker you carmilized it. It will still work carmilized though IME (only tried twice worked both times after PCing for over an hour).




My friend PC'd for 30 mins each time she's done it with no caramelization.  I think what causes it to caramelize is how quickly the PC heats up.  Put it on a lower heat than usual to get it up to the right temp and you should be able to PC it for 30 mins or so.  Not like it really matters, just mentioning it :wink:


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Offlinescatmanrav
Brainy Smurf

Registered: 05/08/04
Posts: 11,483
Loc: Flag
Last seen: 3 years, 10 months
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Re: Liquid mycelium tek, help!! [Re: Leukoism]
    #3218109 - 10/05/04 02:21 PM (12 years, 2 months ago)

With honey water too? Not karo or malt because I've notice honey in particular. And there was no change in color at all? It was just as lightly honey colored as when it went it or did it get darker at all?

It is possible your theory is correct though, I always heat up my PC on high (despite knowing you should heat it up slower according to instructions).

But 15 minutes at 15 PSI is enough to sterilize liquids either way. Gotta make sure you start the timer at 15 PSI too, these times don't include warmup times.


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


Post Extras: Print Post  Remind Me! Notify Moderator
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