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Amazon Shop for: ½ Pint Jars, Laminar Flow Hood, Vermiculite

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OfflineMoneyAddyct
Picaro
Registered: 09/10/04
Posts: 410
Last seen: 6 years, 5 months
Am I screwed??
    #3214994 - 10/04/04 07:31 PM (12 years, 2 months ago)

I innoculated 10 jars today. This is my first time growing. I innoculated in a bathroom which was wiped down with Lysol wipes and sprayed with Lysol and Neutra-Air until you could barely breathe. Two things make me think I might be screwed: (1) The holes I made on my lid were too small to fit the syringe need in, so I had to open the top and innoculate without a lid in place. Will that lead to contamination? (2) I almost ran out of spore solution; therefore, some of my jars have 1-1.5cc of solution while a few have only .75cc of spore solution. Will those with less solution not colonize? Please give me good news.


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OfflineCrazyJulio
journeyman
Registered: 01/29/04
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Re: Am I screwed?? [Re: MoneyAddyct]
    #3215026 - 10/04/04 07:36 PM (12 years, 2 months ago)

1) probably
2) they'll be ok, just slower


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OfflineMoneyAddyct
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Registered: 09/10/04
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Re: Am I screwed?? [Re: MoneyAddyct]
    #3215050 - 10/04/04 07:41 PM (12 years, 2 months ago)

Damn, so either way, whether they grow or not, you think they'll be contaminated? Damn damn damn


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Invisiblemuse_sick
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Re: Am I screwed?? [Re: MoneyAddyct]
    #3215068 - 10/04/04 07:45 PM (12 years, 2 months ago)

hard to tell
it is more likely since the substrate was exposed to the air that it will contaminate
on the other hand i have seen people do G2G transfers w/o a flow hood and they turned out okay for the most part, with a small amount surrendering to contams
my prediction is that you lose some of yr jars, and keep some
good luck all the same
:thumbup:


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:beakedwhale:


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OfflineMoneyAddyct
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Re: Am I screwed?? [Re: MoneyAddyct]
    #3215089 - 10/04/04 07:48 PM (12 years, 2 months ago)

Also, I didn't put the needle into the substrate/verm. I just let the solution drip out of the syringe and onto the substrate/verm mix toward the glass. Was that a big big mistake? Something tells me I should order another spore syringe now.


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Invisiblemuse_sick
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Re: Am I screwed?? [Re: MoneyAddyct]
    #3215102 - 10/04/04 07:50 PM (12 years, 2 months ago)

yes, you should probably go ahead and get another syringe.
unless you have a few CCs left of yours right now...
then do a liquid myc tek


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:beakedwhale:


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OfflineMoneyAddyct
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Registered: 09/10/04
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Re: Am I screwed?? [Re: MoneyAddyct]
    #3215144 - 10/04/04 07:57 PM (12 years, 2 months ago)

Damn. I'm pissed. I'll give it a few days--see what happens. Which is the best supporter to order from?


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OfflineMoneyAddyct
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Re: Am I screwed?? [Re: MoneyAddyct]
    #3215186 - 10/04/04 08:04 PM (12 years, 2 months ago)

Also, how long after innoculation should I begin to see contaminations if they're going to be as abundant as I think they'll be with my innoculation method


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Invisiblemuse_sick
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Re: Am I screwed?? [Re: MoneyAddyct]
    #3215203 - 10/04/04 08:07 PM (12 years, 2 months ago)

no telling how long it might take to see signs
could be tomorrow, could be in two weeks
all shroomery sponsors are great
go to the sponsors forum, ralphster usually has a shroomery special where ya get a free syringe w every order...


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:beakedwhale:


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Offlinezoltar
Hello I love youwont you tell meyour name?

Registered: 09/14/04
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Re: Am I screwed?? [Re: MoneyAddyct]
    #3215271 - 10/04/04 08:33 PM (12 years, 2 months ago)

If I were you what id do is order another spore syringe. But still keep those jars atleast one of those should make it just throw em away as you notice contams. The more jars you did the better a chance you have of atleast one making it. On a grain jar once i fucked up on the tyvec filter and turned out it wasnt on properly i inocced any way and it turned out that it was my best jar so all hope isnt lost. Next time though innoc properly. If your going to do innoc in a bathroom try not to use so much lysol (it can lead to mutants) wipe the walls and all surfaces with a bleach water solution and go kinda light on the lysol dont forget the oust or neutra air though (dont go so heavy on that either). Also wipe whatever surfaces you use with rubbing alcahol before placing the jars on them. It also helps to get some of those dust masks so your not breathing contams all over your nicely cleaned bathroom. And of course make sure your clean as well, shower before you inocc and wear some clean clothes the less the better (i innoc in nothing but a pair of freshly washed boxers). A really well cleaned bathroom can work just as well as a glove box IME.


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Offlineliveby
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Registered: 06/15/04
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Re: Am I screwed?? [Re: MoneyAddyct]
    #3215457 - 10/04/04 09:14 PM (12 years, 2 months ago)

i have dont it that way many time b4!dont worry be happy!


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OfflineMoneyAddyct
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Re: Am I screwed?? [Re: MoneyAddyct]
    #3215468 - 10/04/04 09:18 PM (12 years, 2 months ago)

Ok, so I'm not entirely disheartened. I just ordered a GT spore syringe from Spores101 and selected B+ as my free syringe with my purchase.


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InvisibleNemo_Hoes
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Re: Am I screwed?? [Re: MoneyAddyct]
    #3215503 - 10/04/04 09:26 PM (12 years, 2 months ago)

just put them away and don't touch/look/breathe on them for 2 weeks and you might lose a few


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We will also report to the NAACP and to Al Sharpton's entourage, how the Shroomery administrators allows their mods and members to be balatantly allowed the use of the 'N' word.


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OfflineMushBus
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Registered: 09/09/04
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Re: Am I screwed?? [Re: MoneyAddyct]
    #3215529 - 10/04/04 09:31 PM (12 years, 2 months ago)

does anyone else find not looking at them very hard to do.

my buddy cant stand it and he takes peeks everyday or so.


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Offlineholio1
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Registered: 03/19/04
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Re: Am I screwed?? [Re: MushBus]
    #3215556 - 10/04/04 09:37 PM (12 years, 2 months ago)

yea for the first few days I can go with not looking at em because tehre probably wont be anything there, but once they really get going I check everyday if I am around home.


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InvisibleNemo_Hoes
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Re: Am I screwed?? [Re: holio1]
    #3215575 - 10/04/04 09:40 PM (12 years, 2 months ago)

That's a good way to introduce contams, it's best not to do that, you'll get over it after a few grows.


--------------------
We will also report to the NAACP and to Al Sharpton's entourage, how the Shroomery administrators allows their mods and members to be balatantly allowed the use of the 'N' word.


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InvisibleSam1912
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Registered: 09/23/04
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Re: Am I screwed?? [Re: MoneyAddyct]
    #3215630 - 10/04/04 09:50 PM (12 years, 2 months ago)

How does looking at them introduce them to contam?

I peek at mine every few hours...(first grow, day 5)

Sam


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Invisibleadoseofparn0z
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Re: Am I screwed?? [Re: MoneyAddyct]
    #3215655 - 10/04/04 09:56 PM (12 years, 2 months ago)

It'll only cause contams if you open up the jar to look at the progress, but just looking at it through the glass is fine until it's done colonizing.


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OfflineXanaDude
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Registered: 09/22/04
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Re: Am I screwed?? [Re: MoneyAddyct]
    #3216305 - 10/05/04 12:34 AM (12 years, 2 months ago)

Next time you could build a simple glove box out of a cardboard box, duct tape, and some plastic sheeting. Lysol the crap out of the inside of the box 10 minutes before you use it. Wear gloves while handling objects in the box. That's basically what I do and I've done grain-to-grain transfers opening all kinds of jars and never had contams.

Best of luck!


--------------------
"The Power of Ten" -- the coolest non-drug mindfuck! See it at: http://micro.magnet.fsu.edu/primer/java/scienceopticsu/powersof10/


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OfflineMoneyAddyct
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Re: Am I screwed?? [Re: XanaDude]
    #3226495 - 10/07/04 09:43 AM (12 years, 2 months ago)

I'm wondering...since I never penetrated the dry layer of vermiculite with the needle when I inoculated, I only let the solution drip out towards the side of the jars.....Is it possible that the spore solution got "stuck" in the dry vermiculite and never made it to the substrate/verm. below and therefore won't colonize at all?


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Amazon Shop for: ½ Pint Jars, Laminar Flow Hood, Vermiculite

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