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Offlinesplix
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Registered: 11/18/00
Posts: 43
Last seen: 14 years, 8 months
new early mold & contam testing method?
    #319061 - 05/17/01 03:13 AM (15 years, 6 months ago)

well almost 12 hours have gome by since that first post about the yellow apearence, The mold i want to grow is still growing. but that bright neon yellow color has not grown, it doesnt even look like its something that would grow. more like a yellow spuge of BRF against the glass... Ohh wow.. i just did a test in the middle of writing this. I was think that due to its bright neon/floresent yellow nature that it might glow under a black light. YOU WOULD NOT BELIEVE THE FUCKING GLOW THIS YELLOW WHATEVER IT IS PRODUCES.. Hey, this might be a whole new premiss for early checking of different types of molds and contams?? A Blacklight Tek huh??? I have never read anything about blacklights and growing befor. plenty of use of blacklights while on shrooms though. whatever this yellow stuff is, Man, i cant even come close to describing the way it reacts to this test.. basicly, nothing else in the entire room glows like this. Its a transparent almost non-seeable noen yellow in normal light, but a yellowish orangish bright glowing color under BL.

Hey any of you out there that have blacklights, take a little look at your crop.. see if you have anything out of the ordinary, or unseeable without the blacklight? please post your results..

Has anyone done this befor? lets see where we can take this.???




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Offlinesplix
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Registered: 11/18/00
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Update [Re: splix]
    #319098 - 05/17/01 04:49 AM (15 years, 6 months ago)

UPDATE!!!!!

After writing that post i decided to go give a full exam to all jars,
Man i dont know what this shit is.. 4 jars total have it, 3 of the are smaller than half inch by half inch, on one of them its like 1 inch by 1 inch, and compleatly invisable to the naked eye. but compleatly blinding in glow under black light! i was suprised i saw it on the one jar. these other 3 you cant see a thing under normal light bulb light, or sunlight. but what ever it is sure has a love for Blacklight.

Blacklight Tek anyone???



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Offlinenononsense
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Registered: 03/26/01
Posts: 319
Last seen: 14 years, 11 months
Re: Update [Re: splix]
    #319101 - 05/17/01 04:55 AM (15 years, 6 months ago)

hmm, things that glow up under a blacklight. Orange you said?
One should studie more on spectrums of light!
Why don't try 2 cut it out, and than purify the yellow contam to see what it is!

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OfflineSuntzu
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Re: Update [Re: splix]
    #319320 - 05/17/01 01:28 PM (15 years, 6 months ago)

Sounds like a Pseudomonas infection, likely Pseudomonas fluorescens/putida or similar.

One of the tests to distinguish among species of Pseudomonas IS the black light test; P. aeruginosa will not fluoresce, P. fluorescens [suprise] will.

A coworker told me about this work she did in college [micro major] where they went to the supermarkets and tested a bunch of those chicken breasts in the styrofoam trays with plastic wrap. Just about EVERY ONE of the chicken breasts fluoresced. Cooincidence, you might say, some other compound is responsible. Well they CULTURED these chickens and the skin swabs grew up almost 100% P. fluorescens.
My theory is that it isn't a bad enough bacteria to really cause concern, and likely keeps other potential pathogens' numbers down [e.g. Salmonella]. And since Pse. sp. are all obligate aerobes, they usually stay on the outer surfaces. It's kind of gross, though, a thin 'skin of Pseudomonas' around your meat.

So I think the blacklight test is only good for this kind of contamination. It might pick up other bacteria/maybe mold, as it is only a 'differential' once you've already done some preliminary Pseudomonas ID-ing.

Just for shits and giggles, try the black light on a freshly-opened chicken breast the next time you buy one.

The above information is complete fiction.


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OfflineSuntzu
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Re: Update [Re: Suntzu]
    #319321 - 05/17/01 01:31 PM (15 years, 6 months ago)

One other thing, the color you described is a perfect description of P. fluorescens on agar.

The above information is complete fiction.


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Offlinesplix
newbie
Registered: 11/18/00
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Last seen: 14 years, 8 months
Re: Update [Re: Suntzu]
    #319333 - 05/17/01 01:52 PM (15 years, 6 months ago)

I have no clue what Pseudomonas is, what causes it?

Out of the 10 jars
5 are,
1/8 cup BRF, 1/2 cup Vermic, 45cc water.
the other 5 are
1/4 cup BRF, 1/2 cup Vermic, 60cc water.

its got 3 of the second formula, 1 of the first. I dont have a magnifing glass to look at it close but one thing i can notice is that it does not apear on the vermiculite, only the BRF, It is Glowing BRF. Has not increased in size at all in 24 hours on the one, or on the other 3 in the last 12 hours (1st time i noticed on those) and is still 100% invisable under any normal lighting conditions (Sun, Light Bulb, Ect.) except for black light.

SUNTZU,

you say,
My theory is that it isn't a bad enough bacteria to really cause concern, and likely keeps other potential
pathogens' numbers down.

i was thinking of letting them grow, even if just for the education porpus of it. but you think that might still be safe? later today i will crack the one with the biggest spot of it and give a little smell.



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Offlinesplix
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Registered: 11/18/00
Posts: 43
Last seen: 14 years, 8 months
Re: Update [Re: splix]
    #319608 - 05/17/01 08:46 PM (15 years, 6 months ago)

Well today i cracked the lids on all of them to let them breath a little, they have all been on tight since day 1, which was about 9 days ago. The 4 that are showing have no different smell the the other 6. No sour or contam smell in any of them.

Has anyone else taken a look at thier jars under a blacklight since reading this? If so, have yoou found anything that cant be seen by normal light?



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Offlinesplix
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Registered: 11/18/00
Posts: 43
Last seen: 14 years, 8 months
Re: Update [Re: splix]
    #320887 - 05/19/01 02:28 PM (15 years, 6 months ago)

Well its been a few days know since i noticed anthing. The colored spots have not grown any bigger at all, but they seem to glow brighter now under blacklight.. if this is a bacteria thatt my mold can co-exist with im going to let them grow.. thinking of killing off a jar and sending a sample of to someone that has a lab and can test it for me..

question is, If I open a jar that is only 40-50% colonized and remove everything to get the sample, what are the odds of putting it all back in a jar and having it finish colonization and be a productive member of my grow chamber? on 3 of the jars this coloring is about half inch up from the bottom of the jar, and on 1 it is on the bottom of the jar.



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OfflineSuntzu
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Re: Update [Re: splix]
    #321313 - 05/20/01 01:06 AM (15 years, 6 months ago)

Hey Splix; If you're seriously interested in getting this thing ID'd, SEND IT TO ME.

I just finished working at a bona fide diagnostic microbiology lab, and while I dont' have the media at my apartment or anything, I have a good relationship with my coworkers. In fact, I was going to submit a swab of some bacterial blotch I've been getting lately. I'd bet that both of our culprits are Pseudomonas, but we can get them speciated at no cost to either of us. Here's what you'll need to do;

You could probably get away with wrapping a few small pieces of the contamination in fresh plastic wrap; keep each piece separate to improve chances of viable growth from one. Marble-sized pieces should be more than sufficient. You could also include a Qtip swab of the goo, similarly wrapped.

The downside of getting this done free is that you'll have to take my word on the results, but I can back it up with the various test results.

I doubt you're going to be able to salvage that jar. Maybe, but I doubt it.

PM me if you're interested, I love this pursuit of trivial information! ! !

The above information is complete fiction.


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Offlinesplix
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Registered: 11/18/00
Posts: 43
Last seen: 14 years, 8 months
Re: Update [Re: Suntzu]
    #321826 - 05/20/01 06:59 PM (15 years, 6 months ago)

Where are you located? PM me with your address.




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