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Offlineindigo758
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Registered: 07/01/03
Posts: 114
Last seen: 17 years, 4 months
Wax paper print to syringe
    #3186209 - 09/27/04 12:09 PM (19 years, 4 months ago)

So the second syringe that was made by friend was from a print that came off of wax paper. Had a bitch of a time getting the spores off the wax paper and into the shot glass during the procedure, and is thinking that some wax may have gotten clustered with the spores. This is because when friend looks at the syringe (mexi-cube btw), lots of spores are in little clumps, even when syringe is shaken up. The other syringe that was made with OI spores on foil had spores mostly evenly distributed in the water. Will the clumped spores cause problems when innoculating? Should he maybe innoculate half the amount of jars so it is sure that he will get some growth from that, and just make more prints from the new growth?


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InvisiblePrisoner#1
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Registered: 01/22/03
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Re: Wax paper print to syringe [Re: indigo758]
    #3186221 - 09/27/04 12:13 PM (19 years, 4 months ago)

allow the syringe to rest for a few days and then spend a solid 10 minutes shaking the hell out of it


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Offlineindigo758
psyentist

Registered: 07/01/03
Posts: 114
Last seen: 17 years, 4 months
Re: Wax paper print to syringe [Re: Prisoner#1]
    #3188916 - 09/27/04 10:12 PM (19 years, 4 months ago)

I tried shaking it a bit, but there is no air bubble inside so it only does so well. I read a tek that says to draw sterile air in, put the tip of the needle into a flame and suck air in. I thought this heat may kill the spores... Any more suggestions? I'm still leaning towards inncoulating like 3 or 4 cakes with it so I'm sure to get something.


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InvisiblePrisoner#1
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Re: Wax paper print to syringe [Re: indigo758]
    #3188959 - 09/27/04 10:22 PM (19 years, 4 months ago)

it'll be fine, there wont be enough heat to even warm the water
1/2 cc of air will be plenty for shaking.


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