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Offlinegray1
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Registered: 04/30/01
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Last seen: 16 years, 2 months
antibiotics
    #318469 - 05/16/01 11:21 AM (17 years, 1 month ago)

does anyone have experience with using antibiotics in liquid and agar mycelial culture?
@ what mg/ml concentrations should amp, chloramphenicol, tetracycline, kanamycin, etc... be used at?

c12h16n24ohdmt


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Invisibledimitri211
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Registered: 04/27/01
Posts: 2,248
Re: antibiotics [Re: gray1]
    #318473 - 05/16/01 11:31 AM (17 years, 1 month ago)

Yes I have made my own and purchased it as well pm me and I will tell you more

remember, behind every great fortune there is an even greater crime


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Offlineegghead
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Registered: 04/25/01
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Re: antibiotics [Re: gray1]
    #318486 - 05/16/01 11:54 AM (17 years, 1 month ago)

I have some aquatic antibiotics from the pet store that I'm fully prepared to use if necessary. It's called "Maracin II". Regular "Maracin" has fungicide also, so don't get that.

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Invisibledimitri211
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Registered: 04/27/01
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Re: antibiotics [Re: egghead]
    #318491 - 05/16/01 12:04 PM (17 years, 1 month ago)

unless you are going to put them in after you sterilize you MEA. they will not work they can not withstand the heat of the sterilizer

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OfflineAnnoA
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Re: antibiotics [Re: dimitri211]
    #318498 - 05/16/01 12:19 PM (17 years, 1 month ago)

Gantamicin(gentamycin) sulfate is heat resistant and can be used in agar at a rate of 100 mg/liter of agar medium if bacteria contamination is an issue.

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Offlineegghead
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Re: antibiotics [Re: dimitri211]
    #318500 - 05/16/01 12:27 PM (17 years, 1 month ago)

Agreed. Hopefully there are no fungal spores in the tablets..

So far I've been able to beat out all agar contams through transfers. I know that one day there'll be that clone from the wild that's gonna need an anti-biotic treatment. So it's sittin' here just in case.

* Contributor to the Free Spore Ring


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