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Offlinegray1
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Registered: 04/30/01
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Last seen: 14 years, 7 months
antibiotics
    #318469 - 05/16/01 11:21 AM (15 years, 6 months ago)

does anyone have experience with using antibiotics in liquid and agar mycelial culture?
@ what mg/ml concentrations should amp, chloramphenicol, tetracycline, kanamycin, etc... be used at?

c12h16n24ohdmt


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Invisibledimitri211
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Registered: 04/27/01
Posts: 2,248
Re: antibiotics [Re: gray1]
    #318473 - 05/16/01 11:31 AM (15 years, 6 months ago)

Yes I have made my own and purchased it as well pm me and I will tell you more

remember, behind every great fortune there is an even greater crime


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Offlineegghead
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Registered: 04/25/01
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Re: antibiotics [Re: gray1]
    #318486 - 05/16/01 11:54 AM (15 years, 6 months ago)

I have some aquatic antibiotics from the pet store that I'm fully prepared to use if necessary. It's called "Maracin II". Regular "Maracin" has fungicide also, so don't get that.

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Invisibledimitri211
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Posts: 2,248
Re: antibiotics [Re: egghead]
    #318491 - 05/16/01 12:04 PM (15 years, 6 months ago)

unless you are going to put them in after you sterilize you MEA. they will not work they can not withstand the heat of the sterilizer

remember, behind every great fortune there is an even greater crime


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OfflineAnnoA
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Re: antibiotics [Re: dimitri211]
    #318498 - 05/16/01 12:19 PM (15 years, 6 months ago)

Gantamicin(gentamycin) sulfate is heat resistant and can be used in agar at a rate of 100 mg/liter of agar medium if bacteria contamination is an issue.

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Offlineegghead
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Re: antibiotics [Re: dimitri211]
    #318500 - 05/16/01 12:27 PM (15 years, 6 months ago)

Agreed. Hopefully there are no fungal spores in the tablets..

So far I've been able to beat out all agar contams through transfers. I know that one day there'll be that clone from the wild that's gonna need an anti-biotic treatment. So it's sittin' here just in case.

* Contributor to the Free Spore Ring


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