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Hamurabi
the babylonianleader..
Registered: 03/31/02
Posts: 2,421
Loc: Greece
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pan. cyans
#3182097 - 09/26/04 09:31 AM (19 years, 6 months ago) |
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hi guys, can i innoculate pan.cyans in brf cakes and after they colonise , spawn some poo with them? or i must use grains?
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MAGnum
veteran
Registered: 07/08/04
Posts: 2,421
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Re: pan. cyans [Re: Hamurabi]
#3182114 - 09/26/04 09:47 AM (19 years, 6 months ago) |
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I would use sawdust.
I believe that's what they grow good on.
I have a sawdust tech.
-------------------- Agent 727 7
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Hamurabi
the babylonianleader..
Registered: 03/31/02
Posts: 2,421
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Re: pan. cyans [Re: MAGnum]
#3182139 - 09/26/04 10:08 AM (19 years, 6 months ago) |
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well i didnt want any other teks. i just wanna know if they can be grown with the method i said or not
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scatmanrav
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Registered: 05/08/04
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Re: pan. cyans [Re: Hamurabi]
#3182240 - 09/26/04 10:55 AM (19 years, 6 months ago) |
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Nope I dont think they can. And you need to use agar for them too and isolate a vigorous strain. This is not for a newbie who hasnt mastered cubies (or casings) yet...
Grains and poo *might* work, either way you would need to get an isolate (not using spores to innoculate).
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4hodmt
AspiringMycologist
Registered: 04/06/04
Posts: 759
Last seen: 9 years, 10 months
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what heoppens if u dont get an isolate?
is KARO considered an isolate?
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scatmanrav
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Re: pan. cyans [Re: 4hodmt]
#3182344 - 09/26/04 11:55 AM (19 years, 6 months ago) |
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Karo isnt an isolate unless you used an isolate to knock it up. With pan cyans the growth from multispore is weak (so I hear) and so an isolate (strong rhizo growth) must be gotten using agar or something similar and picking out the best growth. That can then be used (an agar wedge) to knock up a karo jar and let that grow, then your Karo is an isolate. Just making the Karo from multispore though isn't going to isolate any strain traits though, you still have all the crappy myc (from crappy spores) with all the good myc (from good spores). Problem with this is with pan cyans (unlike cubensis which you DONT need an isolate for) there are more bad then good and you end up getting jars that may not fully colonize and even if they do may not fruit. This is all what I've heard but I havn't actually grown pan's so some of that info may not be correct (maybe they really can be done from multispore and the people who I heard talking dont know what they talking bout) but all the isolate stuff (like how to do it and if Karo is an isolate when multispored) is correct I'll search for the other pans cyans thread about this same thing a few days ago and edit my post with the link. Then you can judge for yourselves if you believe the people who say it won't go from multispore.. edit: http://www.shroomery.org/forums/showflat...;o=&fpart=1 There ya go.
Edited by scatmanrav (09/26/04 11:56 AM)
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Snobrdr311
outdoorenthusiast
Registered: 09/03/01
Posts: 1,468
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Quote:
scatmanrav said: Nope I dont think they can. And you need to use agar for them too and isolate a vigorous strain. This is not for a newbie who hasnt mastered cubies (or casings) yet...
Grains and poo *might* work, either way you would need to get an isolate (not using spores to innoculate).
This is BAD information and untrue. You need to use grains with Pan Cyans but you CAN inject spores into a jar of grains with this species! You do NOT need an isolate! You can also fruit them in casings off grains but the preferred method is that grain be spawned to dung or straw.
This species isn't that much harder than cubes if you have already successfully spawned cubes to dung.
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scatmanrav
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Don't forget the rest of what I say in a quote like that instead of taking it out of context. I also said:
"This is all what I've heard but I havn't actually grown pan's so some of that info may not be correct (maybe they really can be done from multispore and the people who I heard talking dont know what they talking bout) but all the isolate stuff (like how to do it and if Karo is an isolate when multispored) is correct I'll search for the other pans cyans thread about this same thing a few days ago and edit my post with the link. Then you can judge for yourselves if you believe the people who say it won't go from multispore..
edit: http://www.shroomery.org/forums/showflat...;o=&fpart=1 There ya go. "
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Snobrdr311
outdoorenthusiast
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No one in the thread you posted said anything about Pan Cyans and them NOT being able to ge grown with multispore injections. You flat out said no they can't.
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Snobrdr311
outdoorenthusiast
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"Nope I dont think they can. And you need to use agar for them too and isolate a vigorous strain. This is not for a newbie who hasnt mastered cubies (or casings) yet..."
WTF is this then?
ANother thing that's wrong, Pan Cyans are NOT much harder to grow than Cubes are long as you can be sterile!! The only harder part is they're more susceptable to contams.. do the sterile procedures by the book and you will have no problems.
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Snobrdr311
outdoorenthusiast
Registered: 09/03/01
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Quote:
scatmanrav said: Don't forget the rest of what I say in a quote like that instead of taking it out of context.
uhm.. that was all you said in the post, and it was your last post here.
"Nope I dont think they can. And you need to use agar for them too and isolate a vigorous strain. This is not for a newbie who hasnt mastered cubies (or casings) yet..."
This is what you said in the whole post, I didn't take anything out of context or forget anything.
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ATWAR
Connoisseur
Registered: 01/26/03
Posts: 1,640
Loc: #108768 in line...
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Re: pan. cyans [Re: MAGnum]
#3182502 - 09/26/04 12:54 PM (19 years, 6 months ago) |
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Quote:
MAGnum said: I would use sawdust.
I believe that's what they grow good on.
I have a sawdust tech.
Time to rethink your species and substrate match... That is not true...
-------------------- To give is to live...
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Alsius
Non-photo Blue
Registered: 07/21/04
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Re: pan. cyans [Re: MAGnum]
#3182505 - 09/26/04 12:54 PM (19 years, 6 months ago) |
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Quote:
MAGnum said: I would use sawdust.
I believe that's what they grow good on.
I have a sawdust tech.
Don't say anything unless you know what the hell you're talking about. Which you never do.
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scatmanrav
Brainy Smurf
Registered: 05/08/04
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http://www.shroomery.org/forums/showflat.php?Number=3170079 Quote from posed: "Pan Cyan's are very vulnerable to contam, which makes the Pan Cyan more difficult. The multispore method is more likely to fail, but is possible." very vulnerable to contam (makes them more difficult then cubies for someone who's only done cakes a few times) is where I got the more difficult then cubies. Also kilgore said: "the myc is weaker i believe, making agar a preferable method so that you can isolate a thick, strong, fast myc strain. that should also help lower contam chances a little " High contams chances from these makes me again say that an isolate should really be used to guarentee any sort of results And nimbus said: "I made some multispore Pan Cyan last spring and out of 8 1 liter trays I only got 1 gram dry" You may not have noticed and just thought this was a succesful grow but 8 1 liter trays got ONE dry gram TOTAL. That means 8 seperate multispore tray's failed. Besides the original poster, and myself and one other person not adding info but asking a question these were the only people who replied. The first two mention that multispore should work but all say negative things about it and then a third saying it didnt work for them (though the myc grew they just didnt fruit) in 8 seperate trays gives me the impression that the thread was against its use. Now for what I said, "nope i dont THINK they can" think being the keyword which I later posted more info when I wasn't in a hurry that I havn't actually grown these. And that was actually the last time I posted (and before your post) that short post wasnt my last one here. So you may have taken what I said in my first post but you still shouldn't leave out that I AM ADMITING I DONT KNOW SHIT ABOUT PAN'S in my second post which was made before you made that post. Now if you'll excuse me I couldn't care less about this crap anymore. I would like to ONCE AGAIN LIKE I DID BEFORE I WAS CORRECTED REPETIDLY SAY I DONT KNOW SHIT ABOUT PAN CYANS other then what I've read. I've read very easy to contam and hard to get to produce fruits when not using an isolate. This makes them IN MY OPINION not something that someone who has only done cakes a few times should do. Damn all I wanted to do was add a little info on isolates...
Edited by scatmanrav (09/26/04 01:19 PM)
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george castanza
Lord Of The Idiots!
Registered: 10/21/02
Posts: 8,749
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Re: pan. cyans [Re: Hamurabi]
#3183109 - 09/26/04 04:00 PM (19 years, 6 months ago) |
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ok hamurabi you got your answer yes.. it should work brf is a grain so you got nothing to worry about.... *click*
-------------------- KRAMER CAKES
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