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InvisibleKOPELANDIAA
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Registered: 11/17/01
Posts: 805
Loc: under a pine
Semilanceata has been cultivated
    #3182147 - 09/26/04 10:14 AM (19 years, 7 months ago)

...and it is not fresh news...but maybe was i the last informed ?
I used to think that nobody ever succeed in attempting to cultivate
this specie in a controled environment...i was wrong.
See:
http://www.psilocybe.org/Wissenschaft/FurtherInvestigation.htm


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OfflineOpenminded
Dicotyledon

Registered: 08/28/03
Posts: 657
Loc: England.
Last seen: 6 years, 4 months
Re: Semilanceata has been cultivated [Re: KOPELANDIAA]
    #3182156 - 09/26/04 10:19 AM (19 years, 7 months ago)

Also, it looks like there's a load of yummy sclerotia in that substrate! Maybe sclerotia could be grown, even if fruiting is too hard for most people?

(or maybe my eyes are playing up, and there are no sclerotia...)

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InvisibleKOPELANDIAA
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Registered: 11/17/01
Posts: 805
Loc: under a pine
Re: Semilanceata has been cultivated [Re: Openminded]
    #3182186 - 09/26/04 10:30 AM (19 years, 7 months ago)

hmm...sclerotia ? With Semilanceata ?
Don't know about this, but since it looks like a temperate cousin
of Mexicana (in appearence only maybe), dreaming is permitted ...

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OfflinePrankster239
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Registered: 01/06/04
Posts: 518
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Re: Semilanceata has been cultivated [Re: KOPELANDIAA]
    #3185400 - 09/27/04 06:03 AM (19 years, 7 months ago)

Semis form sclerotia. Here is a pic from Jochen Gartz`s book " Narrenschw?mme"




Its really not much but i also found this about the semi cultivation in an paper from Gartz:



I have cultivated some fruit bodies of Psilocybe semilanceata to determine
determinate the alkaloid levels in comparison to naturally grown mushrooms.

Mycelium obtained from the spores of one mushroom was kept as a stock
culture on 6% malt agar (6). The spores can germinate after a storage of
the dried mushrooms for 9 months at 20?C.
---------------------------------------------------------------------------
Fig. 2 - Fruiting of Psilocybe semilanceata. [in Erlenmeyer flask]
---------------------------------------------------------------------------

No indole derivatives have been detected in the mycelia from a surface
culture of P. semilanceata on liquid malt extract media. In contrast to
these results a fruiting of the species (figure 2) yielded fruit bodies
with a similar high level of alkaloids as the naturally grown mushrooms
(table 1).

This strain fruited after 3 to 4 months but other mycelia failed to form
fruit bodies at all.


--------------------
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I love to grow exotics with spores from Sporeworks


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InvisibleKOPELANDIAA
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Registered: 11/17/01
Posts: 805
Loc: under a pine
Re: Semilanceata has been cultivated [Re: Prankster239]
    #3185458 - 09/27/04 06:56 AM (19 years, 7 months ago)

wow, thank you...
Would be a good idea to check for sclerotia, next hunt !

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OfflineAlan RockefellerM
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Registered: 03/10/07
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Re: Semilanceata has been cultivated [Re: KOPELANDIAA] * 1
    #27651763 - 02/09/22 03:02 AM (2 years, 3 months ago)

It isn't hard to cultivate Psilocybe semilanceata, but I am pretty sure those are just wild fruits tossed into a erlenmeyer flask.  They look quite a bit different when grown indoors, and would look especially different if fruited in that container.

See https://www.shroomery.org/forums/showflat.php/Number/27613283/fpart/all/vc/1/nt/3

and https://www.shroomery.org/forums/showflat.php/Number/27517472/fpart/all

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OfflineBSUUF2
derails threads
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Registered: 10/15/20
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Re: Semilanceata has been cultivated [Re: Alan Rockefeller] * 1
    #27651791 - 02/09/22 04:43 AM (2 years, 3 months ago)

Congratulations! Now that's some serious thread necroing! :grin:

Any chance getting about four year old semi spores germinated? I've already tried it via agar/inoculation loop (neither myc, mold or bacteria). Maybe swab the print, then cut out some soft agar, put a piece from that swab onto the cut out area, move cut out agar back in place, wait. Worked with a stubborn PE6 swab a year ago (I think about 3 years old), but I'm prioritizing APE now, where I'm in the same situation. I'll be going to try that anyway, once it fits into my time schedule...


--------------------
LAGM2022

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OfflineAlan RockefellerM
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Re: Semilanceata has been cultivated [Re: BSUUF2]
    #27652574 - 02/09/22 04:41 PM (2 years, 3 months ago)

Quote:

BSUUF2 said:
Congratulations! Now that's some serious thread necroing! :grin:

Any chance getting about four year old semi spores germinated? I've already tried it via agar/inoculation loop (neither myc, mold or bacteria). Maybe swab the print, then cut out some soft agar, put a piece from that swab onto the cut out area, move cut out agar back in place, wait. Worked with a stubborn PE6 swab a year ago (I think about 3 years old), but I'm prioritizing APE now, where I'm in the same situation. I'll be going to try that anyway, once it fits into my time schedule...




One of my friends got old Psilocybe semilanceata spores to germinate after putting them directly on agar without any luck.  What he did is add the spores to sterile water in a centrifuge tube, then pipetted some of the spore water onto agar plates every day for a week, spreading the water evenly on the plate with a cell spreader.

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OfflineBSUUF2
derails threads
I'm a teapot User Gallery


Registered: 10/15/20
Posts: 666 666 Posts!
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Re: Semilanceata has been cultivated [Re: Alan Rockefeller]
    #27657381 - 02/13/22 11:18 AM (2 years, 3 months ago)

Quote:

Alan Rockefeller said:
Quote:

BSUUF2 said:
Congratulations! Now that's some serious thread necroing! :grin:

Any chance getting about four year old semi spores germinated? I've already tried it via agar/inoculation loop (neither myc, mold or bacteria). Maybe swab the print, then cut out some soft agar, put a piece from that swab onto the cut out area, move cut out agar back in place, wait. Worked with a stubborn PE6 swab a year ago (I think about 3 years old), but I'm prioritizing APE now, where I'm in the same situation. I'll be going to try that anyway, once it fits into my time schedule...




One of my friends got old Psilocybe semilanceata spores to germinate after putting them directly on agar without any luck.  What he did is add the spores to sterile water in a centrifuge tube, then pipetted some of the spore water onto agar plates every day for a week, spreading the water evenly on the plate with a cell spreader.




Thanks for that info, though I lack this kind of equipment, maybe there's some "ghetto" way...


--------------------
LAGM2022

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