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KOPELANDIAA
Stranger
Registered: 11/17/01
Posts: 805
Loc: under a pine
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Semilanceata has been cultivated
#3182147 - 09/26/04 10:14 AM (19 years, 7 months ago) |
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...and it is not fresh news...but maybe was i the last informed ? I used to think that nobody ever succeed in attempting to cultivate this specie in a controled environment...i was wrong. See: http://www.psilocybe.org/Wissenschaft/FurtherInvestigation.htm
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Openminded
Dicotyledon

Registered: 08/28/03
Posts: 657
Loc: England.
Last seen: 6 years, 4 months
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Re: Semilanceata has been cultivated [Re: KOPELANDIAA]
#3182156 - 09/26/04 10:19 AM (19 years, 7 months ago) |
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Also, it looks like there's a load of yummy sclerotia in that substrate! Maybe sclerotia could be grown, even if fruiting is too hard for most people?
(or maybe my eyes are playing up, and there are no sclerotia...)
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KOPELANDIAA
Stranger
Registered: 11/17/01
Posts: 805
Loc: under a pine
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Re: Semilanceata has been cultivated [Re: Openminded]
#3182186 - 09/26/04 10:30 AM (19 years, 7 months ago) |
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hmm...sclerotia ? With Semilanceata ? Don't know about this, but since it looks like a temperate cousin of Mexicana (in appearence only maybe), dreaming is permitted ...
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Prankster239
Spore Searcher


Registered: 01/06/04
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Loc: Europe
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Re: Semilanceata has been cultivated [Re: KOPELANDIAA]
#3185400 - 09/27/04 06:03 AM (19 years, 7 months ago) |
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Semis form sclerotia. Here is a pic from Jochen Gartz`s book " Narrenschw?mme"

Its really not much but i also found this about the semi cultivation in an paper from Gartz:
I have cultivated some fruit bodies of Psilocybe semilanceata to determine determinate the alkaloid levels in comparison to naturally grown mushrooms.
Mycelium obtained from the spores of one mushroom was kept as a stock culture on 6% malt agar (6). The spores can germinate after a storage of the dried mushrooms for 9 months at 20?C. --------------------------------------------------------------------------- Fig. 2 - Fruiting of Psilocybe semilanceata. [in Erlenmeyer flask] ---------------------------------------------------------------------------
No indole derivatives have been detected in the mycelia from a surface culture of P. semilanceata on liquid malt extract media. In contrast to these results a fruiting of the species (figure 2) yielded fruit bodies with a similar high level of alkaloids as the naturally grown mushrooms (table 1).
This strain fruited after 3 to 4 months but other mycelia failed to form fruit bodies at all.
-------------------- Support www.fsre.nl !!! I love to grow exotics with spores from Sporeworks
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KOPELANDIAA
Stranger
Registered: 11/17/01
Posts: 805
Loc: under a pine
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Re: Semilanceata has been cultivated [Re: Prankster239]
#3185458 - 09/27/04 06:56 AM (19 years, 7 months ago) |
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wow, thank you... Would be a good idea to check for sclerotia, next hunt !
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Alan Rockefeller
Mycologist

Registered: 03/10/07
Posts: 48,392
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Re: Semilanceata has been cultivated [Re: KOPELANDIAA] 1
#27651763 - 02/09/22 03:02 AM (2 years, 3 months ago) |
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It isn't hard to cultivate Psilocybe semilanceata, but I am pretty sure those are just wild fruits tossed into a erlenmeyer flask. They look quite a bit different when grown indoors, and would look especially different if fruited in that container.
See https://www.shroomery.org/forums/showflat.php/Number/27613283/fpart/all/vc/1/nt/3
and https://www.shroomery.org/forums/showflat.php/Number/27517472/fpart/all
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BSUUF2
derails threads



Registered: 10/15/20
Posts: 666
Loc: not that important
Last seen: 2 years, 2 months
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Congratulations! Now that's some serious thread necroing! 
Any chance getting about four year old semi spores germinated? I've already tried it via agar/inoculation loop (neither myc, mold or bacteria). Maybe swab the print, then cut out some soft agar, put a piece from that swab onto the cut out area, move cut out agar back in place, wait. Worked with a stubborn PE6 swab a year ago (I think about 3 years old), but I'm prioritizing APE now, where I'm in the same situation. I'll be going to try that anyway, once it fits into my time schedule...
-------------------- LAGM2022
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Alan Rockefeller
Mycologist

Registered: 03/10/07
Posts: 48,392
Last seen: 2 days, 17 hours
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Re: Semilanceata has been cultivated [Re: BSUUF2]
#27652574 - 02/09/22 04:41 PM (2 years, 3 months ago) |
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Quote:
BSUUF2 said: Congratulations! Now that's some serious thread necroing! 
Any chance getting about four year old semi spores germinated? I've already tried it via agar/inoculation loop (neither myc, mold or bacteria). Maybe swab the print, then cut out some soft agar, put a piece from that swab onto the cut out area, move cut out agar back in place, wait. Worked with a stubborn PE6 swab a year ago (I think about 3 years old), but I'm prioritizing APE now, where I'm in the same situation. I'll be going to try that anyway, once it fits into my time schedule...
One of my friends got old Psilocybe semilanceata spores to germinate after putting them directly on agar without any luck. What he did is add the spores to sterile water in a centrifuge tube, then pipetted some of the spore water onto agar plates every day for a week, spreading the water evenly on the plate with a cell spreader.
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BSUUF2
derails threads



Registered: 10/15/20
Posts: 666
Loc: not that important
Last seen: 2 years, 2 months
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Quote:
Alan Rockefeller said:
Quote:
BSUUF2 said: Congratulations! Now that's some serious thread necroing! 
Any chance getting about four year old semi spores germinated? I've already tried it via agar/inoculation loop (neither myc, mold or bacteria). Maybe swab the print, then cut out some soft agar, put a piece from that swab onto the cut out area, move cut out agar back in place, wait. Worked with a stubborn PE6 swab a year ago (I think about 3 years old), but I'm prioritizing APE now, where I'm in the same situation. I'll be going to try that anyway, once it fits into my time schedule...
One of my friends got old Psilocybe semilanceata spores to germinate after putting them directly on agar without any luck. What he did is add the spores to sterile water in a centrifuge tube, then pipetted some of the spore water onto agar plates every day for a week, spreading the water evenly on the plate with a cell spreader.
Thanks for that info, though I lack this kind of equipment, maybe there's some "ghetto" way...
-------------------- LAGM2022
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