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Amazon Shop: ½ Pint Jars, Agar, Petri Dish

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Offlinenimbus
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Registered: 04/22/04
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AGAR question
    #3178686 - 09/25/04 01:22 PM (13 years, 8 months ago)

I transferred some spores to petri dishes filled with PDY Agar today
and have 2 questions:

- do I have to worry about some condensation of water on the insides
of the lids of the petri dishes?

- do I have to seal them with foil later on? doesnt the mycelium
(later) need any air?

thanks for any answers!


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we are reasonable people...


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Offlineazurescens
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Re: AGAR question [Re: nimbus]
    #3178728 - 09/25/04 01:31 PM (13 years, 8 months ago)

I always turn my dishes upside down to prevent condensation droplets from hitting the agar surface. And I would say most definitely yes to sealing them. YOu can use medical tape or even regular tape. Unless you have them in a clean room that you believe is virtually contam free.


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Offlinenimbus
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Re: AGAR question [Re: azurescens]
    #3178745 - 09/25/04 01:37 PM (13 years, 8 months ago)

hmmm...so I can put them upside down? didnt think of that! :smile: cant see no disadvantage..or is there?

one more question: do I put them in the dark? like jars?


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Offlinescatmanrav
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Registered: 05/08/04
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Re: AGAR question [Re: nimbus]
    #3178977 - 09/25/04 03:22 PM (13 years, 8 months ago)

condensation is fine. You'll be best to wrap them with cling wrap (I cut off the end of a roll to use for just such occasions). Just wrap it around then pull to rip and it sticks right on there. It will allow it to breathe and form a good seal that still comes off easy (easier then tape). I use this and keep plates for over a month before using them and then continue their sycle in the same manner. Hardly ever had contams from outside source (except bad syringe trying to isolate ect, non outside source)


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


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Offlinehyphae
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Re: AGAR question [Re: scatmanrav]
    #3179171 - 09/25/04 04:31 PM (13 years, 8 months ago)

Needs to be Cling Wrap also as other types do not breath like Cling Wrap (gas permeable). The shit works great don't it scatmanrav! Thats the ticket!


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Offlinenimbus
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Re: AGAR question [Re: hyphae]
    #3179515 - 09/25/04 06:27 PM (13 years, 8 months ago)

where do I get that?


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Offlinehyphae
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Re: AGAR question [Re: nimbus]
    #3179569 - 09/25/04 06:38 PM (13 years, 8 months ago)

At the grocery store, Walmart, Kmart, etc; "Cling Wrap" brand.


Edited by hyphae (09/26/04 12:51 AM)


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Offlinescatmanrav
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Registered: 05/08/04
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Re: AGAR question [Re: hyphae]
    #3180994 - 09/26/04 12:38 AM (13 years, 8 months ago)

Yep Cling Wrap only, not just saran wrap! By Reynolds (sp?)...where he said! Works great! Cut a peice off to use for plates and the rest of the roll gets used for covering casings. I've found its best to cover for good myc growth quickly :thumbup:


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


Post Extras: Print Post  Remind Me! Notify Moderator
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Amazon Shop: ½ Pint Jars, Agar, Petri Dish

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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