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lsdwanted
<3 mushies
Registered: 07/03/04
Posts: 76
Loc: Venice Beach, CA
Last seen: 6 years, 2 months
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damnit, try #3 nothing
#3157022 - 09/20/04 05:39 PM (19 years, 5 months ago) |
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well i got 3 jars thank god, not wastin too much verm and brf. Im attemptin to grow sum Cambodians, but its not working to well.There is one problem, i got the temps right at 80-84. i have a rubbermaid tub with an old amp on the bottom with a pretty big towel thats folded up on top of that so the temps dont go to the 90s. (i never bothered to cleean rubbermaid container or amp) THen i have my thermometer on the towel with all the jars with both layers of tinfoil and a the lids on top as well. I do check the jars often wich would problem make like a sudden temperature change, but i doubt thats the reason the mycelium isnt growing. I only PCed for 30 minutes, because well im impatient, but it seemed to cover it and it was pretty low on water. then i put the jars in the freezer to cool em down and imade my three syringes (because theyre very small) and then innoculated my babies. but each time i do innoculate i do get sum spore on top of the tinfoil so maybe thats a problem. Then i put the tinfoil lid back on and the metal lid as well and i stuck em in the incubation chamber. its been 4 days and no signs of growth, im gonna wait a couple more days and these cakes wel go to the same fate as my other ones, ill throw em in my backyard and let them sit and disintergrate in nature :[ if anyone believe i should try other teks, please feel free to suggest, and also i really wanted to check out magash's ziplock tek, but i cant find the post, thanx alot for you time. jeremy
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Edited by lsdwanted (09/20/04 06:01 PM)
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evilchipmunk
All your nutsare belong tome.
Registered: 05/13/04
Posts: 268
Last seen: 16 years, 6 months
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Re: damnit, try #3 nothing [Re: lsdwanted]
#3157260 - 09/20/04 06:43 PM (19 years, 5 months ago) |
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Man, I can tell you exactly what your problem is: impatience. Seriously dude, if you can't even PC for the correct amount of time you're going to have problems from the start. 4 days is too soon to know if you were successful or not, it takes a few days just for the spores to germinate, and then at least a few more days after that to see growth. Put the jars in a dark place and forget about them for a week or so. If you don't see any growth after 2 weeks, then toss them. In the mean time, you should read as many teks and faqs as possible so that hopefully your next attempt will go better. Good luck!
Edited by evilchipmunk (09/20/04 06:44 PM)
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lsdwanted
<3 mushies
Registered: 07/03/04
Posts: 76
Loc: Venice Beach, CA
Last seen: 6 years, 2 months
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yea i figured that was my problem. alright well tahnx for replyin bro, ill wait 2 weeks i guess thats sum good advice.
peace
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shallow
member
Registered: 07/04/04
Posts: 229
Last seen: 15 years, 10 months
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Re: damnit, try #3 nothing [Re: lsdwanted]
#3157390 - 09/20/04 07:19 PM (19 years, 5 months ago) |
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Follow the tek, no improvision, from what I can tell your improvision sucks.
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lsdwanted
<3 mushies
Registered: 07/03/04
Posts: 76
Loc: Venice Beach, CA
Last seen: 6 years, 2 months
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Re: damnit, try #3 nothing [Re: shallow]
#3157508 - 09/20/04 07:45 PM (19 years, 5 months ago) |
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comon dude u can jus say my improvisions arent the greatest, dont say they suck tho :[
haha thanx for being honest bro
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diseasedfetus
I Love You!
Registered: 09/01/04
Posts: 80
Loc: Canada?
Last seen: 19 years, 4 months
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Re: damnit, try #3 nothing [Re: lsdwanted]
#3157523 - 09/20/04 07:49 PM (19 years, 5 months ago) |
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Shallow is a bitch.
-------------------- World of shit in which a sandwich would be what was concerning your fantasic ideals, fruitalicious fuckwad. I am a racoon.
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lsdwanted
<3 mushies
Registered: 07/03/04
Posts: 76
Loc: Venice Beach, CA
Last seen: 6 years, 2 months
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haha thanx
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ShroomAcolyte
Stranger
Registered: 09/16/04
Posts: 73
Loc: Canada
Last seen: 9 years, 5 months
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Re: damnit, try #3 nothing [Re: lsdwanted]
#3157631 - 09/20/04 08:11 PM (19 years, 5 months ago) |
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Quote:
lsdwanted said: well i got 3 jars thank god, not wastin too much verm and brf. Im attemptin to grow sum Cambodians, but its not working to well.There is one problem, i got the temps right at 80-84. i have a rubbermaid tub with an old amp on the bottom with a pretty big towel thats folded up on top of that so the temps dont go to the 90s. (i never bothered to cleean rubbermaid container or amp) THen i have my thermometer on the towel with all the jars with both layers of tinfoil and a the lids on top as well. I do check the jars often wich would problem make like a sudden temperature change, but i doubt thats the reason the mycelium isnt growing. I only PCed for 30 minutes, because well im impatient, but it seemed to cover it and it was pretty low on water. then i put the jars in the freezer to cool em down and imade my three syringes (because theyre very small) and then innoculated my babies. but each time i do innoculate i do get sum spore on top of the tinfoil so maybe thats a problem. Then i put the tinfoil lid back on and the metal lid as well and i stuck em in the incubation chamber. its been 4 days and no signs of growth, im gonna wait a couple more days and these cakes wel go to the same fate as my other ones, ill throw em in my backyard and let them sit and disintergrate in nature :[
if anyone believe i should try other teks, please feel free to suggest, and also i really wanted to check out magash's ziplock tek, but i cant find the post, thanx alot for you time.
jeremy
Ok, if you're still trying for success for a first time, I strongly suggest you follow the Teks to the very best of your ability. Your post is excellently detailed though, so I'll point out some things I suggest you should change. I'm still a newbie grower myself, but I know the Teks very well.
1.) Make extra-sure you have the right water content. Remember, 2 parts verm, 1 part BRF (ground finely), 1 part water. Also, make sure you really cover the tops tightly with foil before you PC, to make sure water doesn't get in OR out.
2.) PC the recommended times Contams are a whore, and with only three jars, make sure you do them absolutely perfectly.
3.) Try to find a larger syringe. They are ridiculously cheap if you can find where to buy them. (Pharmacies, pet stores, farm/ranch type places probably have them.) The more syringes you have to make, the greater the chance of contamination.
4.) I have read in multiple places to never put your jars in the freezer. I really don't know why, I don't think they would crack, and I don't think it would kill the spores, but maybe it would slow them down? Anyways, the point is, follow the Teks. They say don't put them in the freezer, so on your first time, don't put them in the freezer.
I don't mean to sound like an ass or something, just trying to help
I recommend the PF Tek: http://www.shroomery.org/index/par/23593
Good luck, and I hope you succeed with these jars you have now
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Rose
Devil's Advocate
Registered: 09/24/03
Posts: 22,518
Loc: Mod not God
Last seen: 1 year, 7 months
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Re: damnit, try #3 nothing [Re: lsdwanted]
#3157961 - 09/20/04 09:23 PM (19 years, 5 months ago) |
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Follow the tek. You think you are impatient now... wait till you fail 3 more times. Be patient next time... and you will have a shroom stash before you start your next grow.
-------------------- Fiddlesticks.
Edited by Rose (09/20/04 09:23 PM)
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lsdwanted
<3 mushies
Registered: 07/03/04
Posts: 76
Loc: Venice Beach, CA
Last seen: 6 years, 2 months
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Re: damnit, try #3 nothing [Re: lsdwanted]
#3158270 - 09/20/04 10:24 PM (19 years, 5 months ago) |
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thanx so much for all the help you guys and if u think u sound like an asshole, ShroomAcolyte wow i wonder how u r when u think ur nice... :]
i shall tresure this advice.
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scatmanrav
Brainy Smurf
Registered: 05/08/04
Posts: 11,483
Loc:
Last seen: 11 years, 2 months
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Re: damnit, try #3 nothing [Re: lsdwanted]
#3158313 - 09/20/04 10:35 PM (19 years, 5 months ago) |
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Try doing 20 quarts of rye at a time...
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ZeroArmy27
I didn't go to work for a month.
Registered: 08/31/04
Posts: 1,169
Loc: Middle of nowhere
Last seen: 6 years, 19 days
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Re: damnit, try #3 nothing [Re: scatmanrav]
#3158506 - 09/20/04 11:30 PM (19 years, 5 months ago) |
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I don't think he's going to have horrible results if he only PCs for 30 minutes with the PF Tek, people boil for an hour with the PF Tek and get no contamination whatsoever. The really strong need to PC is when you work with grains, where endospores can get on your grains or in your grains, so that 24 hour soak you do before makes them germinate, then the good, long PC will kill them. With verm and rice flour, I don't see too many problems happening.
Did you let your spores rehydrate in the syringe/water for at least 12 hours? I didn't do this on my first attempt and the growth was DRASTICALLY slower than when I hydrated them. If this is the case, try it next time, and learn from your mistakes.
Give the jars a good 2 weeks. If it takes longer than that, your cakes could get dried out or contaminated while you wait for the mycelium to take over the jar...
Hope I helped, and good luck.
Oh, and BTW - if you're going to cool your jars in the freezer, be sure to put them in a sterile box first - a shoebox soaked in Lysol and allowed to dry would work, and then put them in the box and that box in the freezer. Make sure when they're cooling that the tape is attached... when the jars cool, they like to suck air in, bringing in contams. So if you're impatient and want to cool them that way, make sure you cut down the avaliability of those contams. I'm a freezer guy myself, although I've been told it's bad. My jars have never cracked and I've not been contamed in my jars for over a month - my source of contam was an old needle that just wouldn't get cleaned out, even with a PC in with some jars - new needle, no problems.
-------------------- "a monkey would fuck you up if you tried to put it in a autoclave" - Psychoslut "it's not like the admins and mods are a tight-knit group of hippies that spend their life together in a log cabin tie-dying shirts and stringing beads inbetween bonghits." - Wiccan_Seeker
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lsdwanted
<3 mushies
Registered: 07/03/04
Posts: 76
Loc: Venice Beach, CA
Last seen: 6 years, 2 months
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Re: damnit, try #3 nothing [Re: ZeroArmy27]
#3159159 - 09/21/04 08:24 AM (19 years, 5 months ago) |
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that rehydration sounds like a good idea and thanx for that shoe box trick, wow the suggestions keep on coming, you guys are awesome.
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mudvayne
newbie
Registered: 08/19/04
Posts: 64
Last seen: 18 years, 8 months
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Re: damnit, try #3 nothing [Re: lsdwanted]
#3159230 - 09/21/04 09:21 AM (19 years, 5 months ago) |
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you need to increase your pc time by at least 30 minutes and increase your patience. 4 days is way too early to panic.
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Reject
member
Registered: 02/18/04
Posts: 260
Loc: L'ville
Last seen: 15 years, 8 months
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Re: damnit, try #3 nothing [Re: lsdwanted]
#3159322 - 09/21/04 09:53 AM (19 years, 5 months ago) |
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My biggest recommendation is to not put them in the freezer.
Let them cool down from PCing naturally... by putting them in the freezer, you could have a couple of things happen. 1. Outside cools quickly, inside still warm... bam spores are dead 2. Inside and outside get really cool, freeze the water in the jars, spores don't like the jars, get a sudden cold shock which will delay or possibly stop germination.
-------------------- Any and all references to growing are completely facticious, all pictures shown unless specifically stated are stock photographs found via google image search.
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lsdwanted
<3 mushies
Registered: 07/03/04
Posts: 76
Loc: Venice Beach, CA
Last seen: 6 years, 2 months
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Re: damnit, try #3 nothing [Re: Reject]
#3160522 - 09/21/04 03:14 PM (19 years, 5 months ago) |
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thanx for the suggestions i really appreciate all of u taking ur time to help me. Also i got the temps up to 86 and below by putin a towel on top of the tub :]. that will speed up colonization correct?
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lsdwanted
<3 mushies
Registered: 07/03/04
Posts: 76
Loc: Venice Beach, CA
Last seen: 6 years, 2 months
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Re: damnit, try #3 nothing [Re: lsdwanted]
#3160904 - 09/21/04 04:24 PM (19 years, 5 months ago) |
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i kno ur gonna say im impatient, but ithink im going to innoculate the cake again cuz i got a better syringe and im very sure i innoculated whiel the jars were too hot and i didnt let the spore rehydrate at all, so i believe to try again. tell me if thats a dumb idea, thanx
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scatmanrav
Brainy Smurf
Registered: 05/08/04
Posts: 11,483
Loc:
Last seen: 11 years, 2 months
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Re: damnit, try #3 nothing [Re: lsdwanted]
#3161035 - 09/21/04 05:02 PM (19 years, 5 months ago) |
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You'd be better making new substrate and letting it cool overnight after being PCed. Those that you have if they are going to contam have a head start on the spores. If you did the sterilization process correctly then you should be ok injecting them again. There isnt a problem with injecting them twice (other then possibly getting to much water if you already were close to to much) its the fact that theyre being injected almost a week after PCed. I do wait till up to 2 weeks sometimes before knocking up my jars but mine are properly PCed. If you did well enough with that 30 minutes (maybe you did with BRF, I use grains and they need longer) then you should be ok but do you want to risk a good syringe?
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zerozero
Stranger
Registered: 08/31/04
Posts: 782
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Re: damnit, try #3 nothing [Re: scatmanrav]
#3161164 - 09/21/04 05:28 PM (19 years, 5 months ago) |
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yo lsd, i had a problem that was kinda the same and all i can say is what i did. i didn't use my good syringes on old pre-inoculated material cause i didnt want to take the chance of wasting more spores, which are harder to get than grain. so i just left my old stuff and started new making sure i did everything exactly like a tek told me, and that way if i didnt have luck i wouldnt wonder if i should have redone those jars or not. but if you have excess spores then go ahead. peace 00
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Diploid
Cuban
Registered: 01/09/03
Posts: 19,274
Loc: Rabbit Hole
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Re: damnit, try #3 nothing [Re: lsdwanted]
#3161209 - 09/21/04 05:42 PM (19 years, 5 months ago) |
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Man, I can tell you exactly what your problem is: impatience.
Ditto!
-------------------- Republican Values: 1) You can't get married to your spouse who is the same sex as you. 2) You can't have an abortion no matter how much you don't want a child. 3) You can't have a certain plant in your possession or you'll get locked up with a rapist and a murderer. 4) We need a smaller, less-intrusive government.
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zappaisgod
horrid asshole
Registered: 02/11/04
Posts: 81,741
Loc: Fractallife's gym
Last seen: 7 years, 9 months
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Re: damnit, try #3 nothing [Re: Reject]
#3161254 - 09/21/04 05:58 PM (19 years, 5 months ago) |
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Quote:
Reject said: My biggest recommendation is to not put them in the freezer.
Let them cool down from PCing naturally... by putting them in the freezer, you could have a couple of things happen. 1. Outside cools quickly, inside still warm... bam spores are dead 2. Inside and outside get really cool, freeze the water in the jars, spores don't like the jars, get a sudden cold shock which will delay or possibly stop germination.
I put my jars in the freezer after pcing and had no problem. I only gave them a couple hours and I don't have a super duper low temp freezer but I don't think you have to worry about internal temps still being so high that it will kill the spores. After all. you're dripping the spore solution down the outside of the jar. No growth gets near the center for several days.
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lsdwanted
<3 mushies
Registered: 07/03/04
Posts: 76
Loc: Venice Beach, CA
Last seen: 6 years, 2 months
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Re: damnit, try #3 nothing [Re: zappaisgod]
#3161518 - 09/21/04 06:44 PM (19 years, 5 months ago) |
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im goin to reinnoculate, 2morrow morning or tommorrow afternoon when the spores are done rehydrating, any suggestions on how long i should let it sit after it leaves the fridge, and again i reeeaaallly apppreciate your guys responses, i mean wow one of my posts has 2 pages lol :]
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lsdwanted
<3 mushies
Registered: 07/03/04
Posts: 76
Loc: Venice Beach, CA
Last seen: 6 years, 2 months
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Re: damnit, try #3 nothing [Re: Diploid]
#3161536 - 09/21/04 06:47 PM (19 years, 5 months ago) |
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o and would it a b a better idea to just innoculate the old jars. or should i rePC them?
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scatmanrav
Brainy Smurf
Registered: 05/08/04
Posts: 11,483
Loc:
Last seen: 11 years, 2 months
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Re: damnit, try #3 nothing [Re: lsdwanted]
#3164560 - 09/22/04 11:58 AM (19 years, 5 months ago) |
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Hmm tough question...you could do either and I'd probably rePC them since your going to knock them up anyway. Actually I'd get some new jars and see if you really failed on your old ones or just stalled them (poor water content). I NEVER EVER EVER throw out a jar until I see a contam. Took almost 2 weeks for some cabodian spores I had to germinate.
How long you should let what sit after leaving the fridge? Not sure what your taking out of the fridge but nothing should be in there. Untill you mean the syringe in which case you can use them immediatly (didnt think of syringe at first, store mine at room temp in a drawer).
I mean dont forget we are assuming these are your only mistakes, we have no idea if you got the correct water content in your jars or not (which is VERY important). You might keep failing not knowing this is the problem. Thats usually what it is when growth takes a while to start and to go once started.
Oh and you can use the freezer but for someone who has failed mutiple times dont you think it would be best to cut out the shortcuts? That's where you find the problems. I have used the fridge and freezer however I make sure to not leave them in for long and I use grains which can be shaken to make sure the center gets cooled at the same rate.
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Rose
Devil's Advocate
Registered: 09/24/03
Posts: 22,518
Loc: Mod not God
Last seen: 1 year, 7 months
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Re: damnit, try #3 nothing [Re: lsdwanted]
#3164578 - 09/22/04 12:02 PM (19 years, 5 months ago) |
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Perhaps you should try following the advice of your fellow cultivators if you wish to succeed.
-------------------- Fiddlesticks.
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lsdwanted
<3 mushies
Registered: 07/03/04
Posts: 76
Loc: Venice Beach, CA
Last seen: 6 years, 2 months
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Re: damnit, try #3 nothing [Re: lsdwanted]
#3179200 - 09/25/04 02:46 PM (19 years, 5 months ago) |
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hell yea thanx everyone, i think i got mycelial growth on one of the jars in 2 areas, im not sure exactly, but im very sure, ill keep u guys up dated and ill tell u how it is by wednesday prolly.
thanx again, peace
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Killer
Bowhunter
Registered: 09/17/04
Posts: 25
Loc: Wisconsin
Last seen: 19 years, 5 months
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Re: damnit, try #3 nothing [Re: lsdwanted]
#3179208 - 09/25/04 02:48 PM (19 years, 5 months ago) |
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Paitence is a virtue
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lsdwanted
<3 mushies
Registered: 07/03/04
Posts: 76
Loc: Venice Beach, CA
Last seen: 6 years, 2 months
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Re: damnit, try #3 nothing [Re: Killer]
#3179310 - 09/25/04 03:23 PM (19 years, 5 months ago) |
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i concur
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