Home | Community | Message Board


Kraken Kratom
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop for: ½ Pint Jars, Agar, Mycobags

Jump to first unread post. Pages: 1
Offlinejethro
Stranger

Registered: 09/19/04
Posts: 43
Last seen: 11 years, 3 months
Producing Mycellial Syringes.....PMS
    #3154291 - 09/19/04 11:59 PM (12 years, 2 months ago)

What method of making mycelium syringes work the best for you?


-shooting spores into honey water and then sucking up mycellial growth

-taking a tissue culture from the inner meat of the mushroom

-growing cultures on agar and blending to make syringes


Which one produces the least contams.


My friend wants to do mycobags, but until now grain to grain was working fine. Every time he has tried honey water or tissue he has gotten contams. Is H2O2 needed?


Is a contam-free mycellial syringe possible


Edited by jethro (09/20/04 12:01 AM)


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblemuse_sick
ĤŌĿŶĞЋ0$Ŧ

Folding@home Statistics
Registered: 12/15/03
Posts: 9,399
Loc: Giu La Testa
Re: Producing Mycellial Syringes.....PMS [Re: jethro]
    #3154820 - 09/20/04 02:23 AM (12 years, 2 months ago)

IMO the best innovation for the home cultivator is the karo jars
just 100 mL h20/1 tablespoon karo
PC for 10 minutes
let cool,
shoot up with 2ccs of multispore or myc
let colonize
break up myc with a sterile syringe by sucking in and shooting out water
then fill syringe
shoot up substrate
colonization time is drastically cut
h202 is not needed but if you find it helps then i encourage you to experiment at low dilutions
h202 is not needed


--------------------
:beakedwhale:


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinediscman1
journeyman
Registered: 08/24/04
Posts: 962
Last seen: 11 years, 11 months
Re: Producing Mycellial Syringes.....PMS [Re: muse_sick]
    #3154859 - 09/20/04 02:41 AM (12 years, 2 months ago)

Pardon my n00bage, but what is karo?

Links welcomed. :wink:


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblemuse_sick
ĤŌĿŶĞЋ0$Ŧ

Folding@home Statistics
Registered: 12/15/03
Posts: 9,399
Loc: Giu La Testa
Re: Producing Mycellial Syringes.....PMS [Re: discman1]
    #3154888 - 09/20/04 02:48 AM (12 years, 2 months ago)

karo is light corn syrup
available in any supermarket


--------------------
:beakedwhale:


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinediscman1
journeyman
Registered: 08/24/04
Posts: 962
Last seen: 11 years, 11 months
Re: Producing Mycellial Syringes.....PMS [Re: muse_sick]
    #3154955 - 09/20/04 03:16 AM (12 years, 2 months ago)

Ooh, duh. :wink: Interesting. So can you just innoculate the bottle as it comes, since you would assume that it is sterile?

I think I'm in need of a tek, here. I'm interested in this avenue.

Edit: On second look, you do say that you need to PC them. Ok.

As for the H2O2... I suppose it certainly doesen't hurt anything. H2O2 degrades rapidly into H2O and O when heated, so I guess you'll have to be the judge of whether it's worth it or not.

It might be better to add the H2O2 after being PC'd. It would help oxygenate the corn syrup. Adds contam risk, but it might speed up mycelium growth for a few days.

I'm under the assumption that the mycelium grows rather slowly in such liquid substrates. My guess would be due to lack of gas exchange.


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblemuse_sick
ĤŌĿŶĞЋ0$Ŧ

Folding@home Statistics
Registered: 12/15/03
Posts: 9,399
Loc: Giu La Testa
Re: Producing Mycellial Syringes.....PMS [Re: discman1]
    #3155015 - 09/20/04 03:47 AM (12 years, 2 months ago)

no it grows fairly fast
5 days you will have a usable amount of myc
if you incubate


--------------------
:beakedwhale:


Post Extras: Print Post  Remind Me! Notify Moderator
Offlineonetime
onetime
 User Gallery

Registered: 11/14/03
Posts: 3,609
Last seen: 5 years, 11 months
Re: Producing Mycellial Syringes.....PMS [Re: muse_sick]
    #3155043 - 09/20/04 11:07 AM (12 years, 2 months ago)

well one could also scrape spores in to a shot glass and then suck up stairal honey or kero and squirt that into the shot glass and suck it up again and incubate the sryinge and then inject couldnt you ?


--------------------

See?
Yes, with my own three eyes.
Depression, Misspells , wanting everying thing i cant have haveing nothing i want


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinejethro
Stranger

Registered: 09/19/04
Posts: 43
Last seen: 11 years, 3 months
Re: Producing Mycellial Syringes.....PMS [Re: onetime]
    #3155144 - 09/20/04 11:59 AM (12 years, 2 months ago)

I am familiar on how to make syringes, but they always contam. Even with H2O2.

we are talking like ten tries with zero success.


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleMagashM
Da Bud Guru
 User Gallery

Registered: 07/25/02
Posts: 5,875
Loc: Near Hilo
Trusted Cultivator
Re: Producing Mycellial Syringes.....PMS [Re: jethro]
    #3155164 - 09/20/04 12:06 PM (12 years, 2 months ago)

Ok, here is what I did and it's in the faq as the poorman's syringe tek. (it should be the lazy man's tek).

Stick syringes with steril water in em in a glove box. Put in a shotglass and a fully colonized cake.

Well if you know the cake is fully colonized you know it's not contamed. I just shoot the water into the shotglass and pull the plunger out of the syringe. Scrape a little myc off of the cake and put it in the syringe by hand. Put the plunger back in suck up the water and shake like a spaztic monkey before using them. This was tested on over 100 cakes by two different people.  :grin: :thumbup: :wink: :cool:


--------------------
All creatures tremble when faced with violence. All creatures fear death, all love life. If we can only see ourselves in others, then how could we possibly hurt another creature?


:growingweed: Join us at the Growery! :growingweed:


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinejethro
Stranger

Registered: 09/19/04
Posts: 43
Last seen: 11 years, 3 months
Re: Producing Mycellial Syringes.....PMS [Re: Magash]
    #3156759 - 09/20/04 06:28 PM (12 years, 2 months ago)

Thank you, I definately need something that works. Don't wanna waste those bags or all that grain.


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop for: ½ Pint Jars, Agar, Mycobags

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* Shotglass growing
( 1 2 all )
coconutjamushies 2,121 22 06/24/07 11:47 AM
by coconutjamushies
* Liquid Mycellial Culture Question :-) Mklangelo 1,537 8 01/25/02 08:40 AM
by ar393
* Re: Mycellial Slowness (ramsey seal ?) 3Mshroom 567 6 04/18/00 12:51 PM
by Anonymous
* I am scared to do a Print/syring! fearforce7 4,196 16 07/22/12 02:56 PM
by God Stamet
* cakes producing on verm pleezr 566 2 12/25/01 03:55 PM
by sporechase13
* shotglass colonize time? tort 917 18 09/23/01 09:36 AM
by Zen Peddler
* Colonizing using shotglasses Shlewm 671 15 12/04/05 11:45 PM
by xburn
* Extra Syring smkpt 1,332 14 05/07/07 11:26 PM
by

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Magash, Shroomism, george castanza, Prisoner#1, RogerRabbit, Citric, total, FooMan, 13shrooms, stonesun, EvilMushroom666, cronicr, PussyFart
704 topic views. 22 members, 92 guests and 25 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Lil Shop Of Spores
Please support our sponsors.

Copyright 1997-2016 Mind Media. Some rights reserved.

Generated in 0.032 seconds spending 0.003 seconds on 14 queries.