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Offlineqwon
destroying angel
Registered: 08/21/04
Posts: 324
Last seen: 3 years, 7 months
spore printing
    #3152489 - 09/19/04 12:17 AM (12 years, 2 months ago)

I read a few teks about sporeprinting. I have a monster near ready and would like some input from you guys; i.e. teks. References?


--------------------
'I'm the commander see, I don't need to explain, I do not need to
explain why I say things. That's the interesting thing about being the president.
Maybe somebody needs to explain to me why they say something,
but I don't feel like I owe anybody an explanation.'
- George W. Bush


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OfflineZeroArmy27
I didn't go to work for a month.
 Arcade Champion: Asteroids 2000

Registered: 09/01/04
Posts: 1,169
Loc: Middle of nowhere
Last seen: 6 years, 1 month
Re: spore printing [Re: qwon]
    #3152774 - 09/19/04 01:38 AM (12 years, 2 months ago)

just follow the teks on the site, man. it's super easy.

take your cap and put it on some HEAVY DUTY foil, not the thin stuff. make sure it's gills down. put a cup over it where it's slightly cracked to allow moisture to escape. if your cap is slightly dry, try putting a drop or two of water on it.

make sure this is in a relatively clean/draft-free room.


--------------------
"a monkey would fuck you up if you tried to put it in a autoclave" - Psychoslut

"it's not like the admins and mods are a tight-knit group of hippies that spend their life together in a log cabin tie-dying shirts and stringing beads inbetween bonghits." - Wiccan_Seeker


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OfflineSpudz76
Got mycelium on the brain...
Male

Registered: 02/08/04
Posts: 391
Last seen: 6 years, 8 months
Re: spore printing [Re: qwon]
    #3152840 - 09/19/04 01:57 AM (12 years, 2 months ago)

A flowhood or glovebox are the right places to do a print, but if you like risks then do it in a relatively clean and draft-free room. :smile:

Depends on if you want to be really sure the print won't be harboring contams when it comes time to make a syringe, and then you'll be sad that 100% of your inoculations will be contam'd.


--------------------
Anywhere in this posting where I have referred to myself or my possessions, it is a personalization of events and items experienced or possessed by someone else.  I do not personally do anything illegal, and I hope nobody else does either.


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OfflineZeroArmy27
I didn't go to work for a month.
 Arcade Champion: Asteroids 2000

Registered: 09/01/04
Posts: 1,169
Loc: Middle of nowhere
Last seen: 6 years, 1 month
Re: spore printing [Re: Spudz76]
    #3152897 - 09/19/04 02:21 AM (12 years, 2 months ago)

I don't use a glove box spudz, and the only time I got contams was when I didn't sterilize between jars. I've been super clean and smart about everything since.

As long as you're careful, you can get away with not haveing a glove box. I just wouldn't suggest trying to do g2g transfers or other sensitive operations without one.


--------------------
"a monkey would fuck you up if you tried to put it in a autoclave" - Psychoslut

"it's not like the admins and mods are a tight-knit group of hippies that spend their life together in a log cabin tie-dying shirts and stringing beads inbetween bonghits." - Wiccan_Seeker


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OfflinemotamanM
old hand
 User Gallery
Registered: 12/18/02
Posts: 6,021
Last seen: 11 days, 5 hours
Re: spore printing [Re: qwon]
    #3152922 - 09/19/04 02:32 AM (12 years, 2 months ago)



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Invisibleutopianglory
Spunkmuffin
Registered: 07/20/02
Posts: 965
Re: spore printing [Re: qwon]
    #3152923 - 09/19/04 02:32 AM (12 years, 2 months ago)

Spore printing is a fairly forgiving part of the process.


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