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Invisibletahoe
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Ps Cyanescens mycelium Question
    #3151541 - 09/18/04 06:40 PM (12 years, 9 months ago)

Does ps cyan mycel grow rhizomorphicaly on mea? I was looking at a Petri and its lumpy and a tad cottony, no thick rhizos like cubes on agar.


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OfflineAnnoA
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Re: Ps Cyanescens mycelium Question [Re: tahoe]
    #3151586 - 09/18/04 06:54 PM (12 years, 9 months ago)

No rhizos, no. But the growth is rather thick and linear usually, depending on the agar formulation.


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Offlinehyphae
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Re: Ps Cyanescens mycelium Question [Re: tahoe]
    #3151664 - 09/18/04 07:28 PM (12 years, 9 months ago)

I've also noted the linear tomentose type growth which is normal. I've started it on MEA also then to rye--> cardboard--> alder chips. I've had better success with PF style jars with sawdust/wheatbran and a syringe though.


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Offlineshirley knott
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Re: Ps Cyanescens mycelium Question [Re: hyphae]
    #3151668 - 09/18/04 07:29 PM (12 years, 9 months ago)

why so many steps, hyphae?


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Offlinehyphae
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Re: Ps Cyanescens mycelium Question [Re: shirley knott]
    #3151688 - 09/18/04 07:36 PM (12 years, 9 months ago)

Agar for isolation rye for it's many inoculation points for the cardboard then trays were filled halfway with chips the colonized sheet was laid on top of that and the trays were filled with chips for the remaining space. That was the oldtimers way way back when! :wink: Now it's PF style sawdust/bran then these are dumped into large bags of hardwood chips and these are left to colonize during the summer and dumped in late summer, much simpler (KISS). :wink:


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Invisibletahoe
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Re: Ps Cyanescens mycelium Question [Re: hyphae]
    #3157393 - 09/20/04 09:19 PM (12 years, 9 months ago)

cool, Thanks for the info. I new it looked like mycel but i dont have my copy of tmc to refer to. how do these look?


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OfflineJAMIEK
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Re: Ps Cyanescens mycelium Question [Re: tahoe]
    #3157401 - 09/20/04 09:22 PM (12 years, 9 months ago)

What is this method called in ur pic?????? and what is ur medium there honey water?


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OfflineJAMIEK
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Re: Ps Cyanescens mycelium Question [Re: JAMIEK]
    #3157422 - 09/20/04 09:29 PM (12 years, 9 months ago)

Ok petri dish I got that now what is the medium
Im reading hyphae Im reading....
Now when is that spawn ready to be introduce to a medium is it cut into pieces of that petri dish or what is the process plz?
Also is the growth so quick can them petri dishes be used as sort of a mother spawn for cloning sorta....Am I in the ball park here?


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Offlinehyphae
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Re: Ps Cyanescens mycelium Question [Re: JAMIEK]
    #3157489 - 09/20/04 09:41 PM (12 years, 9 months ago)

Cold weather species are rather slow growing compared to warm weather species. The agar media looks to be MEA (malt extract agar). Those dishes tahoe look heavenly BTW! WTG!!!


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Invisibletahoe
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Re: Ps Cyanescens mycelium Question [Re: JAMIEK]
    #3157497 - 09/20/04 09:43 PM (12 years, 9 months ago)

the petri dish is to isolate a strong, healthy strain. i can also isolate contams with it. The spores for this were from a wild specimen


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