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OfflineSporpheus
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Registered: 05/10/01
Posts: 5
Last seen: 23 years, 22 days
How do you use agar?
    #314315 - 05/10/01 08:03 PM (23 years, 22 days ago)

Does anyone have any links to some good agar teks or anything? When using agar do you like let the mycelium grow on the agar and then case the agar with bird seed or whatever and then case that with the vermiculite or what? Also, I heard something about making brown rice flour paste and using it as if it was agar... anyone know anything about that?


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OfflineAnnoA
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Registered: 06/17/99
Posts: 24,166
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Re: How do you use agar? [Re: Sporpheus]
    #314319 - 05/10/01 08:08 PM (23 years, 22 days ago)

Read this:
http://www.mykoweb.com/articles/cultivation.html

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Offlineegghead
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Registered: 04/25/01
Posts: 1,054
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Re: How do you use agar? [Re: Sporpheus]
    #314521 - 05/11/01 05:03 AM (23 years, 22 days ago)

What you described might work just fine. You can get a little more mileage from your agar cultures by slicing them up and dropping the slices into prepared grain jars (1 or 2 slices per jar). Cutting the slices as triangles works well.

Some folks will put the agar culture into a blender w/ sterile water and chop them up into a liquid innoculant. Then they inject that solution into a prepared substrate.

Some folks find working with agar cultures fun & interesting. It's also a good way to work with a dirty print and/or to isolate a strain within a culture. You can get free sample from Moorhead Agar. The sample (two 8 oz packs) should last you a good long time.



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OfflineKaren
Pooh-Bah
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Registered: 01/02/00
Posts: 1,677
Last seen: 7 years, 7 days
Re: How do you use agar? [Re: egghead]
    #315139 - 05/11/01 07:03 PM (23 years, 21 days ago)

I was going to get one of those free samples but seeing as how I am new to this I didn't know which one to pick.

GELIDIUM or GRACILARIA

I have no idea what that means. Can You help me out?

Karen



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OfflineAnnoA
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Re: How do you use agar? [Re: Karen]
    #315171 - 05/11/01 07:48 PM (23 years, 21 days ago)

These are 2 different species of seaweed agar is extracted from.

http://www.mbari.org/~conn/botany/reds/sharon/
http://www.botany.hawaii.edu/GradStud/smith/websites/Gsal-home.htm

Order both samples ;)

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Offlineegghead
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Registered: 04/25/01
Posts: 1,054
Loc: Milky Way
Last seen: 22 years, 2 months
Re: How do you use agar? [Re: Karen]
    #315215 - 05/11/01 08:46 PM (23 years, 21 days ago)

I got both.. ;) Sporeworks is also selling it now if you don't feel like waiting. It took about 10 days for the sample to arrive.




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