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Offlineindigo758
psyentist

Registered: 07/01/03
Posts: 114
Last seen: 18 years, 3 months
Steriling syringes?
    #3119805 - 09/10/04 06:29 PM (20 years, 4 months ago)

I've been trying to find the best way to sterilize my syringes. I was going to try boiling some water and then rinsing with it, but I realized this wouldn't sterilize the needle cover at all so it would be kind of pointless. I was thinking of PCing them, but I'm a lil apprehensive. This'll be the first time I'm actually going to use a PC so i don't know a lot about em. Whats the best way to do this? Thanks.


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please allow me to introduce myself, i'm a man of wealth and taste...

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OfflineAhHaHaHa
there issomething on myshoulder
Registered: 08/29/04
Posts: 199
Loc: upon the east
Last seen: 20 years, 3 months
Re: Steriling syringes? [Re: indigo758]
    #3119815 - 09/10/04 06:33 PM (20 years, 4 months ago)

Burn needle then wipe with alchohol before innoc. I rinse syringe 4 or 5 times with boiling water. Then I put boiling water in and let cool before sucking up spores. Overall the PC is better. Of course you could do both.


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So Im packing my bags for the mysty mountains where the spirits go.

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Offlinescape
II
Registered: 08/03/04
Posts: 315
Loc: somewhere but not where y...
Last seen: 4 years, 11 months
Re: Steriling syringes? [Re: AhHaHaHa]
    #3119854 - 09/10/04 06:48 PM (20 years, 4 months ago)



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EVERYTHING I SAY OR DO IS FAKE, IM A COMPULSIVE LIAR, sorry for any confusion this makes.

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OfflineStrange_Design
Cucumber

Registered: 04/18/04
Posts: 43
Last seen: 17 years, 11 months
Re: Steriling syringes? [Re: scape]
    #3120009 - 09/10/04 07:40 PM (20 years, 4 months ago)

the very simple and highly effective way of doing it goes like this:

place empty syringes (with needle guard on) on a sheet of alum foil and wrap it around them tightly

then place that in your pc, best to use a empty jar to keep them up out of the water, get it up to 15 pounds of pressure and cook for 25 min.

take it out and let cool and you are good to go.

you can also toss your syringes in with any jars you happen to be cooking, just set them on top. the added time won't hurt them abit. i have seen syringes be used 100's of times like this with no probs.

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Invisiblegeorge castanzaM
Lord Of The Idiots!
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Registered: 10/21/02
Posts: 8,777
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Re: Steriling syringes? [Re: Strange_Design]
    #3121777 - 09/11/04 09:47 AM (20 years, 4 months ago)

Quote:

Strange_Design said:
the very simple and highly effective way of doing it goes like this:

place empty syringes (with needle guard on) on a sheet of alum foil and wrap it around them tightly

then place that in your pc, best to use a empty jar to keep them up out of the water, get it up to 15 pounds of pressure and cook for 25 min.

take it out and let cool and you are good to go.

you can also toss your syringes in with any jars you happen to be cooking, just set them on top. the added time won't hurt them abit. i have seen syringes be used 100's of times like this with no probs.


:thumbup:


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KRAMER CAKES



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Offlinehyphae
born to grow
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Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 13 years, 8 months
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Re: Steriling syringes? [Re: Strange_Design]
    #3121836 - 09/11/04 10:24 AM (20 years, 4 months ago)

Quote:

Strange_Design said:
the very simple and highly effective way of doing it goes like this:

place empty syringes (with needle guard on) on a sheet of alum foil and wrap it around them tightly

then place that in your pc, best to use a empty jar to keep them up out of the water, get it up to 15 pounds of pressure and cook for 25 min.

take it out and let cool and you are good to go.

you can also toss your syringes in with any jars you happen to be cooking, just set them on top. the added time won't hurt them abit. i have seen syringes be used 100's of times like this with no probs.


Just a sidenote on syringes some brands will do well with repeated autoclaving (B-D) Where as some will not (Terumo).

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Offlineindigo758
psyentist

Registered: 07/01/03
Posts: 114
Last seen: 18 years, 3 months
Re: Steriling syringes? [Re: hyphae]
    #3137068 - 09/14/04 11:20 PM (20 years, 4 months ago)

Well just a note to everyone so maybe you can learn from my errors. I didn't realize the amount of water necessary for the PC and just put a little in the bottom. I PC'd for about 10 min at 15 PSI (once i got it to work right), and the steam stopped coming out because there was no more water inside. I cooled down and added more water after reading up a little more (about an inch). I then proceeded to cook them wait til it heated up again (15 min), put the rocker on, let pressurize, and cook for about 25 min. I let the PC cool and proceeded to boil some distilled water i had for the spore solution. After 10 min of boiling i unwrapped the syringes from the foil and what do ya know- melted all to shit. So now i guess i'm goin down to the needle exchange and maybe a tack/feed store to try to get some more syringes, which is just going to be a bitch. So be forewarned~ use caution if you choose to PC your syringes.


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please allow me to introduce myself, i'm a man of wealth and taste...

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Offlinescatmanrav
Brainy Smurf

Registered: 05/08/04
Posts: 11,483
Loc: Flag
Last seen: 12 years, 14 days
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Re: Steriling syringes? [Re: indigo758]
    #3139220 - 09/15/04 03:01 PM (20 years, 4 months ago)

Do you need to use distilled water when making spore syringes? How about liquid myc syringes? Just regular tap water work? Drinking water?


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"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay

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Invisiblegeorge castanzaM
Lord Of The Idiots!
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Registered: 10/21/02
Posts: 8,777
Trusted Cultivator
Re: Steriling syringes? [Re: scatmanrav]
    #3139487 - 09/15/04 04:22 PM (20 years, 4 months ago)

Quote:

scatmanrav said:
Do you need to use distilled water when making spore syringes? How about liquid myc syringes? Just regular tap water work? Drinking water?




1)no,but it doesn't hurt
2)no,but it doesn't hurt

tap water will work,distilled water is like $.59 a gal.... :eek:


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KRAMER CAKES



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Offlineindigo758
psyentist

Registered: 07/01/03
Posts: 114
Last seen: 18 years, 3 months
Re: Steriling syringes? [Re: george castanza]
    #3139960 - 09/15/04 05:55 PM (20 years, 4 months ago)

the syringes i had were monoject for anyone wondering. i got some new ones at the local non big name feed store, but the needles are only an inch or so. will these work for jars?


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please allow me to introduce myself, i'm a man of wealth and taste...

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