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 MoneyAddyct
Picaro
Registered: 09/10/04
Posts: 410
Last seen: 13 years, 10 months
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 Quick Incubation Question 
#3130003 - 09/13/04 02:16 PM (19 years, 7 months ago) |
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During incubation, should the tiny syringe holes in the top of the jar lid that were used for innoculation be covered?
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 fastfred
Old Hand
 
Registered: 05/17/04
Posts: 6,899
Loc: Dark side of the moon
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Re: Quick Incubation Question [Re: MoneyAddyct]
#3130033 - 09/13/04 02:24 PM (19 years, 7 months ago) |
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Yes, the entire top of the jar should be covered by tinfoil. The holes should not be taped shut.
-FF
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zdm
Baby eater
Registered: 08/01/04
Posts: 457
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Re: Quick Incubation Question [Re: MoneyAddyct]
#3130101 - 09/13/04 02:42 PM (19 years, 7 months ago) |
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read it wrong
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Edited by zdm (09/13/04 02:42 PM)
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ZeroArmy27
I didn't go to work for a month.
Registered: 08/31/04
Posts: 1,169
Loc: Middle of nowhere
Last seen: 6 years, 1 month
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Re: Quick Incubation Question [Re: MoneyAddyct]
#3130189 - 09/13/04 03:10 PM (19 years, 7 months ago) |
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This is actually something that some people argue about. I keep mine closed until there's very noticable growth, then take the foil/tape off to help with gas exchange so my colonization will go as fast as possible. I'm sure the foil won't hinder the gas exchange enough to hurt you, but I'd take the tape off if it were me.
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"a monkey would fuck you up if you tried to put it in a autoclave" - Psychoslut
"it's not like the admins and mods are a tight-knit group of hippies that spend their life together in a log cabin tie-dying shirts and stringing beads inbetween bonghits." - Wiccan_Seeker
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ZeroArmy27
I didn't go to work for a month.
Registered: 08/31/04
Posts: 1,169
Loc: Middle of nowhere
Last seen: 6 years, 1 month
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Re: Quick Incubation Question [Re: ZeroArmy27]
#3130201 - 09/13/04 03:16 PM (19 years, 7 months ago) |
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Oh, I forgot something. I have that layer of vermiculite on the tops of my jars, so that will help stop contaiminants. I also put tyvek between the ring and the lid (I have 2-piece lids), that allows gas exchange while adding another barrier for those nasty contams. You can pick up Tyvek at your local post office in the form of Priority or Express mail envelopes, just look for the word Tyvek on them. It may say 25% post-consumer content, but that's just recycled Tyvek that Du Pont uses... not paper as some people seem to think. You should consider the Tyvek for your next batch of jars.
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"a monkey would fuck you up if you tried to put it in a autoclave" - Psychoslut
"it's not like the admins and mods are a tight-knit group of hippies that spend their life together in a log cabin tie-dying shirts and stringing beads inbetween bonghits." - Wiccan_Seeker
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kitteh
yeehaw
Registered: 03/08/03
Posts: 134
Loc: SC
Last seen: 11 years, 11 months
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Re: Quick Incubation Question [Re: MoneyAddyct]
#3130216 - 09/13/04 03:21 PM (19 years, 7 months ago) |
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I have found that it really doesnt matter if you tape up the innculation points or not using PFTEK. I havent noticed a diffrence so therefore if i tape the holes i keep them taped. I HAVE noticed if you dont put dry verm ALL the way to the top the jar it is more likely to contam.
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hyphae
born to grow
Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 12 years, 10 months
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Re: Quick Incubation Question [Re: kitteh]
#3130268 - 09/13/04 03:44 PM (19 years, 7 months ago) |
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If your jars are sealed air tight you will drastically increase your chances for unhealthy growth or/and stalled growth, I myself have seen tape stall jars many many times so you kitteh are one of the lucky ones or possibly your jars aren't sealed as tight as you thought.  BTW foil will provide plenty of gas exchange, just don't seal it skin tight! 
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MoneyAddyct
Picaro
Registered: 09/10/04
Posts: 410
Last seen: 13 years, 10 months
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Re: Quick Incubation Question [Re: hyphae]
#3130297 - 09/13/04 03:55 PM (19 years, 7 months ago) |
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Ok. That's all I was worried about--gas exchange. I won't seal the holes with tape, but I will cover the lid with foil (but not too tight). Thank you.
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AhHaHaHa
there issomething on myshoulder
Registered: 08/29/04
Posts: 199
Loc: upon the east
Last seen: 19 years, 6 months
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Re: Quick Incubation Question [Re: kitteh]
#3130373 - 09/13/04 04:13 PM (19 years, 7 months ago) |
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I disagree with the PF tek. I did 24 jars and taped the holes and got 2 jars to grow. Then on the next generation I did Magash tek with a 2 holes covered with tyvek and in about a week all 12 jars were growing mycelia. So I would say gas exchange is important. I had 1 jar with green mold but it is better to have 11 jars growing than no growth at all.
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So Im packing my bags for the mysty mountains where the spirits go.
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fastfred
Old Hand
Registered: 05/17/04
Posts: 6,899
Loc: Dark side of the moon
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Re: Quick Incubation Question [Re: AhHaHaHa]
#3130470 - 09/13/04 04:36 PM (19 years, 7 months ago) |
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>I disagree with the PF tek.
You didn't do the PF tek. PF tek doesn't include taping the holes. Next time you decide to badmouth a tek try doing it right first.
-FF
Edit: I was wrong, live it up while you can.
Edited by fastfred (09/13/04 06:38 PM)
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AhHaHaHa
there issomething on myshoulder
Registered: 08/29/04
Posts: 199
Loc: upon the east
Last seen: 19 years, 6 months
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Re: Quick Incubation Question [Re: fastfred]
#3130910 - 09/13/04 06:18 PM (19 years, 7 months ago) |
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Easy there fastfred, I think you should read the first line under incubation of jars in the PF tek. The original version, not the simple mind.
"After inoculation of the jars, tighten the lid bands and retape the needle holes."
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So Im packing my bags for the mysty mountains where the spirits go.
Edited by AhHaHaHa (09/13/04 06:21 PM)
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hyphae
born to grow
Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 12 years, 10 months
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Re: Quick Incubation Question [Re: fastfred]
#3130924 - 09/13/04 06:20 PM (19 years, 7 months ago) |
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fastfred
Old Hand
Registered: 05/17/04
Posts: 6,899
Loc: Dark side of the moon
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Re: Quick Incubation Question [Re: hyphae]
#3131039 - 09/13/04 06:48 PM (19 years, 7 months ago) |
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Damn, I was wrong. Thanks for pointing that out AhHaHaHa. Now that I look at it... I don't think I ever read it very closely. It wasn't free back when I started, so I used the MMGG which was claimed to be based directly off of PF tek.
Now that I look at it I see that it doesn't even show tinfoil being used. You're right PF tek does suck. MMGG method is the way to go with PF jars.
Sorry AhHaHaHa.
-FF
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AhHaHaHa
there issomething on myshoulder
Registered: 08/29/04
Posts: 199
Loc: upon the east
Last seen: 19 years, 6 months
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Re: Quick Incubation Question [Re: fastfred]
#3131451 - 09/13/04 08:18 PM (19 years, 7 months ago) |
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The PF tek for simple minds uses tin foil. Too much tin foil. Tin foil just doesnt seem sterile to me. I use a grain substrate with tyvek filters. Gas exchange is greater and is conducted through a filter that is very sterile, since it is PC with the jars. Quicker colonization time. More economical also with more nutritonal substrate. I paid maybe 12 dollars at the mill for a 60lb bag of rye grain. The rye expands when boiled so not much is used. A little more work but I think it is worth it. Going on 2 yrs on 1 bag. Check out Magash's rye tek, you might like it.
Apology accepted.
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So Im packing my bags for the mysty mountains where the spirits go.
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fastfred
Old Hand
Registered: 05/17/04
Posts: 6,899
Loc: Dark side of the moon
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Re: Quick Incubation Question [Re: AhHaHaHa]
#3132216 - 09/13/04 10:27 PM (19 years, 7 months ago) |
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>Tin foil just doesnt seem sterile to me.
That's why you put it on the jars and then put it through the pressure cooker.
-FF
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MoneyAddyct
Picaro
Registered: 09/10/04
Posts: 410
Last seen: 13 years, 10 months
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Re: Quick Incubation Question [Re: fastfred]
#3132670 - 09/13/04 11:49 PM (19 years, 7 months ago) |
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Jars should be incubated in the dark, correct? I know exactly where I'll incubate them. I have this one-tier step-stool that has a built-in toolbox/storage area currently with nothing in it. It'll fit 10 jars with no problem and it's completely dark inside. Since my room is usually between 70-80F, the inside of the "toolbox" should be just right.
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fastfred
Old Hand
Registered: 05/17/04
Posts: 6,899
Loc: Dark side of the moon
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Re: Quick Incubation Question [Re: MoneyAddyct]
#3132784 - 09/14/04 12:15 AM (19 years, 7 months ago) |
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Yes, in the dark.
-FF
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wds
another brick
Registered: 03/06/04
Posts: 422
Loc: Mother Earth
Last seen: 16 years, 7 months
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Re: Quick Incubation Question [Re: fastfred]
#3132880 - 09/14/04 12:39 AM (19 years, 7 months ago) |
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i have my holes covered with a layer of tinfoil, would you recommend exposing those four wholes? growth is well on it's way.
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I wish psychedelic mushrooms were legal, because I would like to indulge in this amazing fruit of the earth. Unfortunately, I can only learn about this interesting hobby, and cultivate legal mushrooms.
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fastfred
Old Hand
Registered: 05/17/04
Posts: 6,899
Loc: Dark side of the moon
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Re: Quick Incubation Question [Re: wds]
#3133010 - 09/14/04 01:42 AM (19 years, 7 months ago) |
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Always keep the tinfoil over the top of the jar. The tinfoil is not airtight and will let gas exchange happen. The tinfoil prevents contams from dropping down through the holes.
-FF
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wds
another brick
Registered: 03/06/04
Posts: 422
Loc: Mother Earth
Last seen: 16 years, 7 months
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Re: Quick Incubation Question [Re: fastfred]
#3133028 - 09/14/04 01:53 AM (19 years, 7 months ago) |
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could someone explain how flipping the jars helps? when should i do it?
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I wish psychedelic mushrooms were legal, because I would like to indulge in this amazing fruit of the earth. Unfortunately, I can only learn about this interesting hobby, and cultivate legal mushrooms.
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