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Offlineragadinks
MrBeatle
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Registered: 10/20/03
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How to collect Coprinus comatus spores ?
    #3125334 - 09/12/04 09:38 AM (13 years, 8 months ago)

I have found some shaggy mane and wonder how it is possible to collect their spores ?
When I put them on a piece of paper the whole mushroom turns to black ink ...


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InvisibleSpeeker

Registered: 02/11/04
Posts: 688
Re: How to collect Coprinus comatus spores ? [Re: ragadinks]
    #3146852 - 09/17/04 03:41 AM (13 years, 8 months ago)

1. use a mushroom that is just about to open.
2. pull the leg off.
3. hang the mushroom somehow over a paper.
(I used thin bladed knife that I pushed through the cap
and hung the mushroom with it over a kettle)
4. wait, but not too long (mine was over night <8 h)

the cap starts to open and "melt" but with a bit luck you should be able to get a nice print of it.

:goodluck:


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Offlineragadinks
MrBeatle
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Re: How to collect Coprinus comatus spores ? [Re: Speeker]
    #3147076 - 09/17/04 06:00 AM (13 years, 8 months ago)

Woow, thanks.
Have to try that.
Have you put the spores already on agar ?
Wonder it that works ...


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InvisibleSpeeker

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Re: How to collect Coprinus comatus spores ? [Re: ragadinks]
    #3147109 - 09/17/04 06:17 AM (13 years, 8 months ago)

>Have you put the spores already on agar ?
Not yet. I placed a mushroom piece on agar though ..


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Offlineragadinks
MrBeatle
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Re: How to collect Coprinus comatus spores ? [Re: Speeker]
    #3147131 - 09/17/04 06:29 AM (13 years, 8 months ago)

hope it will take - keep us updated and good luck.


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Offlineragadinks
MrBeatle
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Re: How to collect Coprinus comatus spores ? [Re: Speeker]
    #3174102 - 09/24/04 10:32 AM (13 years, 7 months ago)

thanks speeker for your tip - have tried it out now and it worked very well:



Another way to collect the spores of Coprinus comatus I have found here:

Quote:

You might be able to collect & dry spores from C. comatus by removing a =
chunk of the
black gooey tissue from the bottom of the cap, putting it in 10% chlorox =
solution (9 parts water with 1 part chlorox) and pouring the mess =
through filter paper. The paper could then be dried in a freshly washed =
Mason jar with a filter disk in the lid for ventilation. Scrape up some =
of the dried crust and streak it across an agar plate when you want to =
germinate the spores.

I don't know how well the spores of this species would survive contact =
with the chlorox bath. But those spores that lie protected beneath the =
surface of the cap material would be insulated from contact with the =
bath. From my experience with other species, you can get viable and =
uncontaminated dried spores by this technique.




Btw.: What is chlorox ?


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