Home | Community | Message Board


Vaposhop
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop: ½ Pint Jars, 10cc Syringe, Laminar Flow Hood

Jump to first unread post. Pages: 1
Offlinecarnage
MycoVore
Registered: 02/25/03
Posts: 23
Loc: OHIO
Last seen: 7 years, 11 months
dark syringe preparation
    #3120544 - 09/10/04 11:41 PM (13 years, 1 month ago)

i obtained one of the dark syringes from thelittleguy.com that have lots and lots os spores in them, but i needed to know how to prepare them for inoculation. a friend of mine told me i could get up to 50 10cc syringes from the dark syringe and he told me to put the whole syringe in a jar and draw it out with empty syringes, but how do i know how much water to add? i am confused on how to make lots of syringes from the dark syringe...anybody that has done this before and knows what to do please pass on your knowledge. thanks!


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineJuan_del_Pueblo
Mr.Papashroom
 User Gallery

Registered: 06/26/04
Posts: 585
Last seen: 11 years, 8 months
Re: dark syringe preparation [Re: carnage]
    #3120569 - 09/10/04 11:47 PM (13 years, 1 month ago)

Just follow the Karo tek bro. Peace...


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineAhHaHaHa
there issomething on myshoulder
Registered: 08/30/04
Posts: 199
Loc: upon the east
Last seen: 13 years, 9 days
Re: dark syringe preparation [Re: carnage]
    #3120665 - 09/11/04 12:09 AM (13 years, 1 month ago)

I always make my syringes dark. I think it is assurance of germination within my substrate because of the amount of spores within the injection. I wouldnt make more syringes just cause of contams. I would inject my jars and know that they will colonize. Then t ake a lot of prints on the results and make more syringes on the next generation. But you are not me, so you decide.


--------------------
So Im packing my bags for the mysty mountains where the spirits go.


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleAdden
Buttermilk Love Nugget
I'm a teapot User Gallery

Registered: 06/04/03
Posts: 38,323
Loc: Amongst the Dunes
Re: dark syringe preparation *DELETED* [Re: AhHaHaHa]
    #3120682 - 09/11/04 12:13 AM (13 years, 1 month ago)

Post deleted by S_2

Reason for deletion: This post was made by someone who isn't me.



--------------------


Outdoor Mushroom Cultivation
"He who seeks revenge best dig two graves.."
The Official Woodlovers Thread


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineGr0wer
always improving
Male

Registered: 09/16/03
Posts: 6,039
Loc: El Paso, TX Flag
Last seen: 3 hours, 35 minutes
Trusted Cultivator
Re: dark syringe preparation [Re: Adden]
    #3120943 - 09/11/04 01:24 AM (13 years, 1 month ago)

The karo tek takes the spores and gives then something to grow on so you no longer growing from spores but live cultures and they multiply in volume in the karo. You only need a few cc to innoc a karo jar. 1-3cc would work fine for innoc. That way you can make multiple karo jars and have some left of the spore syringe to fall back on.

I think your friend is saying he dilutes dark syringes by dividing it among other syringes. The best way to innoc from best to worse is G2G, Karo, dark syringes, then clear syringes. Make sure you have a polyfill filer on your karo jar but you can just go from spore if your a virgin mycologist.


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibleutopianglory
Spunkmuffin
Registered: 07/20/02
Posts: 965
Re: dark syringe preparation [Re: Gr0wer]
    #3121054 - 09/11/04 01:59 AM (13 years, 1 month ago)

Most people, most experienced people, including vendors and even vendors who sell dark syringes here think there is no difference between light and dark syringes.

Not trying to start a debate, I'm just telling it like it is. If you are down on printstock, then don't make a dark syringe thinking you have to. If you have plenty, by all means.. do it if it makes you feel more confident.


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinephucknut68
Focker
Registered: 12/08/01
Posts: 141
Loc: shit mang
Last seen: 12 years, 9 months
Re: dark syringe preparation [Re: utopianglory]
    #3121454 - 09/11/04 07:20 AM (13 years, 1 month ago)

I have had some bad luck with clear syringes, whereas dark ones are always good........ these are vendor bought syringes not homemade, either.


--------------------
-=-=-=-=-|:---- Doctor Of Journalism -----:|-=-=-=-=-
-=-=-=-=-|:---- S M C -----:|-=-=-=-=-




Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblefastfred
Old Hand
Male User Gallery

Folding@home Statistics
Registered: 05/17/04
Posts: 6,899
Loc: Dark side of the moon
Re: dark syringe preparation [Re: phucknut68]
    #3121462 - 09/11/04 07:33 AM (13 years, 1 month ago)

Simple math would seem to indicate that darker syringes are a better value... More spores per the dollar.

I'm sure it wouldn't make that much difference, but I would imagine that darker syringes would perform slightly better.


-FF


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibleutopianglory
Spunkmuffin
Registered: 07/20/02
Posts: 965
Re: dark syringe preparation [Re: fastfred]
    #3121480 - 09/11/04 08:06 AM (13 years, 1 month ago)

As I pointed out in another thread, in science there is a concept called saturation. The point at which no further gain can be had with increasing the input. This concept is used heavily in electronics. It is reasonable to suggest that this will occur with germination. There is only so much substrate after all.

One thing that we can all testify to is that if you inject more solution you definitely get a "gain" in performance. It makes more sense for you people to be using more solution that more concentrated solution as you are able to deliver your spores to a larger area of substrate to germinate on and thus avoid the redundancy of injecting too many spores onto an area of host.

Obviously water content can be upset doing this but that probably won't be an issue unless you're trying to go too overboard.

If people didn't believe in this they wouldn't shake their jars to distribute the solution to a larger area as it is.


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleMESCALATO
hunting.
 User Gallery

Registered: 05/16/02
Posts: 1,025
Loc: Central Alabama
Re: dark syringe preparation [Re: carnage]
    #3121518 - 09/11/04 08:45 AM (13 years, 1 month ago)

>a friend of mine told me i could get up to 50 10cc syringes from the dark syringe and he told me to put the whole syringe in a jar and draw it out with empty syringes, but how do i know how much water to add? i am confused on how to make lots of syringes from the dark syringe...

just follow any of the teks for making a syringe from a print. but instead of scraping spores from a print into the shot glass ( or whatever is used ) put 1cc from the dark syringe in to the glass and suck it up into a clean syringe with 9cc of sterile water.


--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblefastfred
Old Hand
Male User Gallery

Folding@home Statistics
Registered: 05/17/04
Posts: 6,899
Loc: Dark side of the moon
Re: dark syringe preparation [Re: utopianglory]
    #3121521 - 09/11/04 08:52 AM (13 years, 1 month ago)

Good point utopianglory. Thank you for taking the time to explain your statement rather than flame me like hyphae does.

One advantage of darker syringes might be that you could use less spore solution when starting liquid culture. Instead of 1cc you might do just as well with 1 drop. I like to have a dozen or so spores germinate and let them battle it out. It makes them fight to get into that syringe you pull out. (odds wise, based on mycelium size at least)

-FF

P.S. Any chemistry minded folks please check out my "psilocin oxidation" thread in advanced cultivation.


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinehawksapprentice
Yearns to Snowboard
 User Gallery

Registered: 06/06/03
Posts: 3,193
Loc: Oregon
Last seen: 1 year, 1 month
Re: dark syringe preparation [Re: carnage]
    #3121525 - 09/11/04 08:56 AM (13 years, 1 month ago)

Okay since noone is really going to awnser the question with detail i guess i will. Sterilize a 250ml jar. Or anyother container that you could sterilize and will hld water. Inject the syringe filled with spores into the container. Now take some 10cc syringes that have been sterilized with 9cc water in them. Shoot that water into the container and suck back up. And repeat for each syringe. Remember to do this in a EXTREMELY sterile environment. But the suggestion of growing them out and printing the best fruits is a good way to go, Also a karo tek is awesome and very easy to do. So with that and all the other posts you should be able to get what you want. Good luck.


--------------------
"I celebrate the Earth, my home, my mother, my grave, and as long as men are Man they must, if they would preserve the integrated being, do the same---[and preserve]--this rank casual hungry smelly sweaty lusting transitory body, my oozy pulpy liquid-bag-swollen body, bones, blood, hair glands, my bejeweled sex; I love and celebrate it all.  never to let men forget that they are animals as much as gods---that is one thing I shall say."

  Edward Abbey


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblefastfred
Old Hand
Male User Gallery

Folding@home Statistics
Registered: 05/17/04
Posts: 6,899
Loc: Dark side of the moon
Re: dark syringe preparation [Re: hawksapprentice]
    #3121527 - 09/11/04 09:03 AM (13 years, 1 month ago)

You could also just add water to the jar first and then sterilize. That might work better if you are using fresh pre-sterilized syringes from a package. Might also have less chance of contams since you are only sucking instead of squirting then sucking repeatedly.


-FF


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinehawksapprentice
Yearns to Snowboard
 User Gallery

Registered: 06/06/03
Posts: 3,193
Loc: Oregon
Last seen: 1 year, 1 month
Re: dark syringe preparation [Re: fastfred]
    #3121542 - 09/11/04 09:23 AM (13 years, 1 month ago)

Good point magash. I jsut figure alot of people are resterilizing the their syringes, i have found this to be a decent way to do it. I usually perform this in a very high quality flow hood. But i think your way is excellent.


--------------------
"I celebrate the Earth, my home, my mother, my grave, and as long as men are Man they must, if they would preserve the integrated being, do the same---[and preserve]--this rank casual hungry smelly sweaty lusting transitory body, my oozy pulpy liquid-bag-swollen body, bones, blood, hair glands, my bejeweled sex; I love and celebrate it all.  never to let men forget that they are animals as much as gods---that is one thing I shall say."

  Edward Abbey


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineMycoCat
a.k.a. ShroomCat

Registered: 10/09/03
Posts: 1,042
Last seen: 3 years, 9 months
Re: dark syringe preparation [Re: hawksapprentice]
    #3122852 - 09/11/04 05:53 PM (13 years, 1 month ago)

I happened to notice this while I was browsing through Ralphster's site, just thought I'd put it out there:

What's the difference between a dark syringe and a regular crystal syringe?

For the longest time I tried to convince customers that a darker syringe with more spores packed into it wasn't necessarily better. We did extensive testing and could not come to the conclusion that one was better than the other. But still - email after email requesting dark syringes. So, I gave in and offer them now purely for novelty. I would suggest sticking to the regular crystal syringes.

- www.ralphstersspores.com


--------------------
No question is so difficult to answer as that to which the answer is obvious.

Meow.


Edited by MycoCat (09/11/04 05:54 PM)


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibleutopianglory
Spunkmuffin
Registered: 07/20/02
Posts: 965
Re: dark syringe preparation [Re: carnage]
    #3124091 - 09/12/04 12:36 AM (13 years, 1 month ago)

Even vendors word, the people who sell dark syringes never does much to quash this  rumour :smile:


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop: ½ Pint Jars, 10cc Syringe, Laminar Flow Hood

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* one syring per print with this tek? ismokeweed 2,384 13 02/06/08 01:06 AM
by Cyber
* Dark Syringes? lobster989 1,103 17 10/21/03 04:05 PM
by
* Really Dark PF Jars...? DrugFreeMan 374 2 07/08/06 05:51 PM
by DrugFreeMan
* How do I prepare to make a Spore Print?????? Cracka_X 1,783 15 07/15/03 05:40 PM
by Ekstaza
* newbie first injection/shelfing questions
( 1 2 all )
deathw 2,834 22 12/17/08 11:02 AM
by SyMaNtiCX66
* Getting ready to make my first spore syring fromthemoon 7,241 10 06/29/07 10:49 PM
by wikedferret
* Newbie about to dive in head first - amount of spore syring CCs into 4 lb grow bag?
( 1 2 all )
ChrisHippyLoser 11,894 25 06/02/16 07:47 PM
by OldSawbonesHisself
* Did my pet lemming inject too much!? DarkLemming 814 5 10/08/02 11:20 PM
by unsecreto

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Roadkill, Magash, Shroomism, george castanza, RogerRabbit, Citric, total, FooMan, 13shrooms, stonesun, cronicr, PussyFart, Tmethyl, Pastywhyte, bodhisatta
1,149 topic views. 32 members, 128 guests and 25 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Azarius
Please support our sponsors.

Copyright 1997-2017 Mind Media. Some rights reserved.

Generated in 0.032 seconds spending 0.004 seconds on 19 queries.