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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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OfflineGulf_Shroomer
Tastes LikeChicken

Registered: 04/06/01
Posts: 103
Last seen: 20 years, 9 months
syringe sterilization?
    #311435 - 05/07/01 03:10 PM (24 years, 1 month ago)

what is the best way to sterilize a spore syringe? (please tell me step by step, as if you have just innoculated your pf jars and are getting ready to put the syringe away.)

Please Help!

--------------------------------------------------------------
Abandon All Ye Hope... For Hell Is An Obstruction Of all Reason...

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OfflineI_Sore
member
Registered: 04/23/01
Posts: 60
Last seen: 23 years, 11 months
Re: syringe sterilization? [Re: Gulf_Shroomer]
    #311462 - 05/07/01 03:41 PM (24 years, 1 month ago)

Heres what I did when I made them for AFOAF:

1. Take them completey apart
2. Rinse all the unused spores out. If you need to, take a needle and scrape them out.
3. Put all the parts in a bowl of rubbing alcohol for a few minutes. Not too long since alcohol weakens plastics. Learned that from cleaning acrylic bongs ;)
4. Boil some water. Then let the parts boil in that for a bit.
5. At this time I use germ X to clean my hands. No use wasting all that sterilzation if you have shit on your hands.
6. Take the parts and shake the water out. Then put them back together.
7. Leave the water boiling. Suck some boiling water into the syringes, and the squirt it into the sink. Do this several times.
8. Suck some more boiling water into the syringe until its full. Put the cap back on it, and place into a baggy. Then another one.

-When you are ready to make syringes from prints use this:

http://www.shroomery.org/findorgrowthem.php?View=docs&doc=119

This isnt 100 percent fail proof. But Ive never had problems with it,.....Maybe its just 100 percent fail proof for me. Ive heard of folks having problems, but this is as good as it gets with used syringes!

-peace



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Second star to the right, and straight on 'till morning..........Let us choke down some of this fairy dust and we are coming peter!

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InvisibleBrownPastures
old hand

Registered: 03/15/01
Posts: 968
Loc: here
Re: syringe sterilization? [Re: I_Sore]
    #311468 - 05/07/01 03:48 PM (24 years, 1 month ago)

great post I sore ... quick and easy .. the best way

go fish

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OfflineGulf_Shroomer
Tastes LikeChicken

Registered: 04/06/01
Posts: 103
Last seen: 20 years, 9 months
Re: syringe sterilization? [Re: I_Sore]
    #311475 - 05/07/01 03:52 PM (24 years, 1 month ago)

im not talking about after you use ALL of the spore liquid, im talking about if i still have like 8 cc's left in the syringe and i just want it sterile so i can get rid of all contams for the next time i want to make a PF cake...

--------------------------------------------------------------
Abandon All Ye Hope... For Hell Is An Obstruction Of all Reason...

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OfflineI_Sore
member
Registered: 04/23/01
Posts: 60
Last seen: 23 years, 11 months
Re: syringe sterilization? [Re: Gulf_Shroomer]
    #311501 - 05/07/01 04:33 PM (24 years, 1 month ago)

hahaha. I m sorry ;)

Just take a lighter and heat the tip when you are ready to inoc again. Once its heated, take some rubbing alcohol on a paper towel and wipe of the needle!

Sorry dude I thought you had finished them off and wanted to use them again! :)

Take it easy!



--------------------
Second star to the right, and straight on 'till morning..........Let us choke down some of this fairy dust and we are coming peter!

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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