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Amazon Shop for: ½ Pint Jars, Brown Rice Flour, Pressure Cooker, Vermiculite

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Offlinemarshmellow
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Registered: 05/09/04
Posts: 20
Last seen: 12 years, 2 months
Little Newb Help Plz
    #3094707 - 09/05/04 01:13 PM (12 years, 3 months ago)

Hello, I made 9 jars up a week or so ago.. 5 pesa and 4 hawiian and the 5 pesa contaminated with green fungi...the hawiian that were done at the same time dont seem to have any... so what I am asking is..do syringes sometimes have bad batches...or is this probably the fault of moi. Also are there any little common newb things that I may be doing? Thanks for any replys.


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Sometimes it seems like such a hard life.
But there?s good times around the bend.
The rollercoasters got to roll to the bottom.
If you want to climb to the top again.


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Invisiblemuse_sick
ĤŌĿŶĞЋ0$Ŧ

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Registered: 12/15/03
Posts: 9,399
Loc: Giu La Testa
Re: Little Newb Help Plz [Re: marshmellow]
    #3094711 - 09/05/04 01:15 PM (12 years, 3 months ago)

sometimes syringes can be bad
if you got it from a vendor, let them know what happened and im sure you will get a replacement
post more details about your sterilization process, the substrate you were using, innoculation procedure, etc and we can help you out more


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:beakedwhale:


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Offlinemarshmellow
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Registered: 05/09/04
Posts: 20
Last seen: 12 years, 2 months
Re: Little Newb Help Plz [Re: muse_sick]
    #3094727 - 09/05/04 01:19 PM (12 years, 3 months ago)

ok.. i use verm brf, boil steam it for 2 hrs, let it cool for 45 min, use a achohol flame before first innoc and rub down the needle with an achohol pad, i rub the syringe with a pad every time but i only flame sterilyze the first hole.


--------------------
Sometimes it seems like such a hard life.
But there?s good times around the bend.
The rollercoasters got to roll to the bottom.
If you want to climb to the top again.


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Offlineshirley knott
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Re: Little Newb Help Plz [Re: marshmellow]
    #3094738 - 09/05/04 01:22 PM (12 years, 3 months ago)

only cooled for 45 minutes?
hmmmm. this was not long enough.


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buh


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Invisiblefastfred
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Re: Little Newb Help Plz [Re: marshmellow]
    #3094760 - 09/05/04 01:28 PM (12 years, 3 months ago)

Try a pressure cooker. Boiling is a waste of time IMO.


-FF


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Invisiblemadsickpenguin
newbie
Registered: 08/29/04
Posts: 234
Re: Little Newb Help Plz [Re: marshmellow]
    #3095109 - 09/05/04 03:14 PM (12 years, 3 months ago)

yea i'm new as well but i've read over and over that 4+ hours is needed for cooling time.


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Invisiblefastfred
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Re: Little Newb Help Plz [Re: madsickpenguin]
    #3095127 - 09/05/04 03:22 PM (12 years, 3 months ago)

No, not that long. Put a fan on it, won't take long at all.


-FF


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OfflineMAGnum
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Registered: 07/08/04
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Re: Little Newb Help Plz [Re: madsickpenguin]
    #3095132 - 09/05/04 03:23 PM (12 years, 3 months ago)

Pressure cook for an hour. Make sure all the substrait is wetted (if the flour is dry, it will not sterilize that section leading to contam). You should have no contam at all if you follow those.

Sometimes syringes get contammed (ask for a replacement saying every jar of that syrine was contammed). Never suck air into the syringe.

The cooling time is best over night.


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Agent 727
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Invisiblefastfred
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Re: Little Newb Help Plz [Re: MAGnum]
    #3095154 - 09/05/04 03:31 PM (12 years, 3 months ago)

MAGnum, he's using brf, so probably also 1/2 pint PF jars, so 1 hour is way too long.

PF says 20 min.


-FF


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Offlinehyphae
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Re: Little Newb Help Plz [Re: fastfred]
    #3095186 - 09/05/04 03:42 PM (12 years, 3 months ago)

Much respects to the professor but your better off closer to 30-45min. IMO always better to be safe than sorry remember core temp of your jars won't heat up to temp instantly :wink: shit takes time! Like I said better safe than sorry, especially with high endospore counts because ya never know!


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Invisiblefastfred
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Re: Little Newb Help Plz [Re: hyphae]
    #3095241 - 09/05/04 03:57 PM (12 years, 3 months ago)

How many jars have you cooked for 20 min that contamed before birth?

30min is a 50% increase and will be plenty, even if you want to be over cautious.

I know you're not dissin the prof, but give him some credit! I'm sure he's probably cooked more pf jars than all of us combined! I think it's the only method he used, I'm pretty sure he worked out a very precise time and already added a safety margin. Since he was really good about replacing syringes I doubt that he would do anything to increase the failure rate.


-FF


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Offlinehyphae
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Re: Little Newb Help Plz [Re: fastfred]
    #3095312 - 09/05/04 04:17 PM (12 years, 3 months ago)

The professor hasn't even come anywhere close to doing as many jars as the GP but I will say his time has got to be close to a minimum effective time my suggested times will work also without any problems what so ever, and BTW I do give him alot of credit and back him up often if ya haven't noticed :wink: Also people have been growing shrooms long before the professor, I for one started 15yrs. before he officially came out with the PFTek in 1991. It's all good why argue? Again GL guys


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OfflineSamhainJ
I wanna rock out, in my dreams
Registered: 05/16/04
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Re: Little Newb Help Plz [Re: hyphae]
    #3100294 - 09/06/04 09:00 PM (12 years, 3 months ago)

my friend only cools for like 40 mins, 20 on the counter, 20 in the freezer, works fine. also if you do not have an air bubble in your syringe to shake it, put the tip in a flame and you can suck a little air in.


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Amazon Shop for: ½ Pint Jars, Brown Rice Flour, Pressure Cooker, Vermiculite

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