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Amazon Shop for: ½ Pint Jars, HEPA Filter, Laminar Flow Hood, Wild Bird Seed

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Offlinejphilly
Stranger
Registered: 11/09/03
Posts: 24
Last seen: 12 years, 4 months
grain to grain
    #3075167 - 08/31/04 08:11 PM (12 years, 4 months ago)

I innoculated about 2 weeks ago using EQ, 7 pint jars using WBS. 1 jar is at about 80%, 2 at about 50%, one at about 25%. I was wondering if i could do a grain to grain tranfer to the other jars that werent showing any progress or if i should just do other jars from scratch (pressure cook and all) and then do a G2G.
what should i do with the jars showing no progress at all...take out of incubator?
Thanks


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InvisibleMykey
spectraltraveler

Registered: 04/07/04
Posts: 542
Re: grain to grain [Re: jphilly]
    #3075193 - 08/31/04 08:19 PM (12 years, 4 months ago)

Give them a good shake to distribute the myc and wait it out another week or so and see what happens before you count them out. It sometimes takes a little longer than usual,but you should be fine unless it takes so long that bacteria begins to show up. It seems like this is what usually gets the slow growing wbs jars.

Good luck!


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Invisiblemuse_sick
ĤŌĿŶĞЋ0$Ŧ

Folding@home Statistics
Registered: 12/15/03
Posts: 9,399
Loc: Giu La Testa
Re: grain to grain [Re: jphilly]
    #3075220 - 08/31/04 08:25 PM (12 years, 4 months ago)

you should shake them like mykey said
then ask your self:
did you innoc down the sides of the jar?
&
shake after you innoced?


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InvisibleFucknuckle
Dog Lover

Registered: 04/24/04
Posts: 6,762
Re: grain to grain [Re: jphilly]
    #3075393 - 08/31/04 09:35 PM (12 years, 4 months ago)

Grain to grain transfers can only be done in front of a flow hood.
If you attempt to open uncolinized jars they will fail.

You would do better to wait. It sounds like over the next 3-4 days they will take off. That is, if the temps are a constent 86 degs????


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What it is, is what it is my Brother.
It is as it is, so suffer thru it.


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InvisibleMykey
spectraltraveler

Registered: 04/07/04
Posts: 542
Re: grain to grain [Re: Fucknuckle]
    #3075405 - 08/31/04 09:39 PM (12 years, 4 months ago)

...or a glovebox.


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InvisibleFucknuckle
Dog Lover

Registered: 04/24/04
Posts: 6,762
Re: grain to grain [Re: Mykey]
    #3075423 - 08/31/04 09:43 PM (12 years, 4 months ago)

Even a glove box will fail unless it has a hepa air filter attached forcing postive air pressure into the glove box. :wink:

Gloveboxs without a hepa air filter attached, in my opion, are only good for doing prints and syringes. :thumbup:


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What it is, is what it is my Brother.
It is as it is, so suffer thru it.


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Offlinemockeylock
head
 User Gallery

Registered: 02/07/04
Posts: 1,046
Last seen: 6 years, 6 months
Re: grain to grain [Re: Mykey]
    #3075840 - 08/31/04 11:53 PM (12 years, 4 months ago)

Quote:

Mykey said:
...or a glovebox.





:thumbup: :mushroom2: :thumbup:


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Amazon Shop for: ½ Pint Jars, HEPA Filter, Laminar Flow Hood, Wild Bird Seed

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