Ah Crap... Frozen spores? Please help - Mushroom Cultivation

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SpaceSpore
stranger thanyou

Registered: 08/14/04
Posts: 29
Last seen: 19 years, 9 days

Ah Crap... Frozen spores? Please help

#3057797 - 08/27/04 11:27 AM (19 years, 7 months ago)

I had syringes of Equador, Mazapetec, Aussie, and Cambodians that I didn't need to use for a few days so I put em in the fridge. I checked back a few hours later and the liquid inside wasn't moving anymore and the little air bubbles inside were frozen in place.

Are the syringes ruined???

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Mushroom spores can survive space travel... imagine the possibilities.

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meanoldman
old guy

Registered: 06/14/04
Posts: 96
Last seen: 3 years, 10 months

Re: Ah Crap... Frozen spores? Please help

[Re: SpaceSpore]
#3057907 - 08/27/04 11:52 AM (19 years, 7 months ago)

Only one way to tell

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SpaceSpore
stranger thanyou

Registered: 08/14/04
Posts: 29
Last seen: 19 years, 9 days

Re: Ah Crap... Frozen spores? Please help

[Re: meanoldman]
#3057924 - 08/27/04 11:57 AM (19 years, 7 months ago)

If I injected them into a mycobag and they didn't take, would it ruin the substrate or could I re-innoculate?

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Mushroom spores can survive space travel... imagine the possibilities.

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Prisoner#1
Even Dumber ThanAdvertized!

Registered: 01/22/03
Posts: 193,665
Loc: Pvt. Pubfag NutSuck

Re: Ah Crap... Frozen spores? Please help

[Re: SpaceSpore]
#3058028 - 08/27/04 12:20 PM (19 years, 7 months ago)

you could re-inocculate... just dont use more than 1cc and make sure that you maintain sterility.

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California Has Legalized Child Prostitution

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george castanza
Lord Of The Idiots!

Registered: 10/21/02
Posts: 8,759

Re: Ah Crap... Frozen spores? Please help

[Re: SpaceSpore]
#3058061 - 08/27/04 12:26 PM (19 years, 7 months ago)

kramer has frozen syringes before and they always worked out fine :crazy2:

if the syringe was good before you froze it then your still gold

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KRAMER CAKES

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psilocyber
old hand

Registered: 06/08/99
Posts: 1,839
Last seen: 1 year, 4 months

Re: Ah Crap... Frozen spores? Please help

[Re: SpaceSpore]
#3058070 - 08/27/04 12:28 PM (19 years, 7 months ago)

As long as nothing else starts growing in your MycoBag (ie molds or other contaminations) then you can always go back and add more inoculation material to them to get them moving again, you don't ruin a bag because of a single injection that doesn't take.

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psilocyber!
SporeWorks: Exotic Mushroom Culture and Spore Supply

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hyphae
born to grow

Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 12 years, 10 months

Re: Ah Crap... Frozen spores? Please help

[Re: SpaceSpore]
#3058341 - 08/27/04 01:32 PM (19 years, 7 months ago)

First thing I'd do is adjust that fridge them give them a try. they can often times handle light freezes but the colder it gets 20F., 10F.,0F.,-10F.,etc; the likely hood of survival decreases for cubies. Your fridge I bet is 30F. but should be 40F. or so :wink:

GL and Welcome SpaceSpore!

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KaptKid
Spaced Pirate

Registered: 12/11/03
Posts: 6,252
Loc: Bright Side of the Sun
Last seen: 4 years, 1 month

Re: Ah Crap... Frozen spores? Please help

[Re: SpaceSpore]
#3058687 - 08/27/04 03:06 PM (19 years, 7 months ago)

Keep my refeg at 50f.It's just for spores.Keep trying.Substrate is cheap(if home made) compaired to bought syringes.

Let us know how it goes

Good Luck

--------------------
Child of the 60's, Tripping ever since.

:sun:

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation

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