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OfflineSpaceSpore
stranger thanyou
Registered: 08/14/04
Posts: 29
Last seen: 11 years, 7 months
Ah Crap... Frozen spores? Please help
    #3057797 - 08/27/04 01:27 PM (12 years, 3 months ago)

I had syringes of Equador, Mazapetec, Aussie, and Cambodians that I didn't need to use for a few days so I put em in the fridge. I checked back a few hours later and the liquid inside wasn't moving anymore and the little air bubbles inside were frozen in place.

Are the syringes ruined???


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Mushroom spores can survive space travel... imagine the possibilities.


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Offlinemeanoldman
old guy
Registered: 06/14/04
Posts: 85
Last seen: 12 years, 2 months
Re: Ah Crap... Frozen spores? Please help [Re: SpaceSpore]
    #3057907 - 08/27/04 01:52 PM (12 years, 3 months ago)

Only one way to tell


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OfflineSpaceSpore
stranger thanyou
Registered: 08/14/04
Posts: 29
Last seen: 11 years, 7 months
Re: Ah Crap... Frozen spores? Please help [Re: meanoldman]
    #3057924 - 08/27/04 01:57 PM (12 years, 3 months ago)

If I injected them into a mycobag and they didn't take, would it ruin the substrate or could I re-innoculate?


--------------------
Mushroom spores can survive space travel... imagine the possibilities.


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InvisiblePrisoner#1M
Even Dumber ThanAdvertized!
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Registered: 01/22/03
Posts: 191,640
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Re: Ah Crap... Frozen spores? Please help [Re: SpaceSpore]
    #3058028 - 08/27/04 02:20 PM (12 years, 3 months ago)

you could re-inocculate... just dont use more than 1cc and make sure that you maintain sterility.


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Invisiblegeorge castanzaM
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Re: Ah Crap... Frozen spores? Please help [Re: SpaceSpore]
    #3058061 - 08/27/04 02:26 PM (12 years, 3 months ago)

kramer has frozen syringes before and they always worked out fine :crazy2:
if the syringe was good before you froze it then your still gold


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Quote:

pftek said:Impossible to clean everything right now. Maybe in a week.





ROTHBURY

:pm:


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OfflinepsilocyberV
old hand

Registered: 06/09/99
Posts: 1,809
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Re: Ah Crap... Frozen spores? Please help [Re: SpaceSpore]
    #3058070 - 08/27/04 02:28 PM (12 years, 3 months ago)

As long as nothing else starts growing in your MycoBag (ie molds or other contaminations) then you can always go back and add more inoculation material to them to get them moving again, you don't ruin a bag because of a single injection that doesn't take.


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Offlinehyphae
born to grow
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Re: Ah Crap... Frozen spores? Please help [Re: SpaceSpore]
    #3058341 - 08/27/04 03:32 PM (12 years, 3 months ago)

First thing I'd do is adjust that fridge them give them a try. they can often times handle light freezes but the colder it gets 20F., 10F.,0F.,-10F.,etc; the likely hood of survival decreases for cubies. Your fridge I bet is 30F. but should be 40F. or so :wink: GL and Welcome SpaceSpore!


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OfflineKaptKid
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Registered: 12/11/03
Posts: 6,247
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Re: Ah Crap... Frozen spores? Please help [Re: SpaceSpore]
    #3058687 - 08/27/04 05:06 PM (12 years, 3 months ago)

Keep my refeg at 50f.It's just for spores.Keep trying.Substrate is cheap(if home made) compaired to bought syringes.

Let us know how it goes

Good Luck


--------------------
Child of the 60's, Tripping ever since.


:sun:


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