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InvisibleBolwarra
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Registered: 06/28/03
Posts: 247
Loc: Australia
Re: hormones gartz used? *brassinosteroid* [Re: Hippie3]
    #3057151 - 08/27/04 07:57 AM (19 years, 7 months ago)

QUOTE: Hippie3 has this doc been moved or ?
Looks like, Anno posted that url over 2 years ago.

Here's another link to the same paper.
--> http://annonymer.members.easyspace.com/G...be_cubensis.pdf

Currently working anyway.


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InvisibleATWAR
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Registered: 01/26/03
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Re: hormones gartz used? *brassinosteroid* [Re: mycofile]
    #3057454 - 08/27/04 09:54 AM (19 years, 7 months ago)

I am curious if anyone has found a natural source that meets these two criteria yet:

1. Brassinosteroids are in a concentration high enough to be beneficial
2. The source is cost effective


I remember reading about this a long time ago, but the main downfall was the price. When I searched I think the cheapest was $200+ a gram. A bit too expensive to experiment with in my lab. And the natural sources that were listed then had concentrations too low to be of use (I think rape seed pollen was one of them)...

Any new developments in this area?


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OfflineBlue Helix
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Re: hormones gartz used? *brassinosteroid* [Re: ATWAR]
    #3141656 - 09/15/04 11:06 PM (19 years, 6 months ago)

Bee pollen does, in my experience, have a positive effect on the speed of colonization. Bee pollen granules (at a rate of about a cup per ten pounds dry substrate) mixed with poo resulted in a much more rhizomorphic growth pattern than normal. Mycellium rhizomorphs rose straight up off the surface, prior to the casing, to a height of about 1/2 inch in some places! Since I don't bother with clones, my observations are not particularly scientific, but considering bee pollen granules run about $10 a pound online, it's not really a big deal to add it.

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OfflineSpudz76
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Registered: 02/08/04
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Re: hormones gartz used? *brassinosteroid* [Re: Blue Helix]
    #3141947 - 09/16/04 12:19 AM (19 years, 6 months ago)

Also found isolated brassinolide source.

here


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Anywhere in this posting where I have referred to myself or my possessions, it is a personalization of events and items experienced or possessed by someone else.  I do not personally do anything illegal, and I hope nobody else does either.

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OfflineFirstAvailable
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Re: hormones gartz used? *brassinosteroid* [Re: Spudz76]
    #3142769 - 09/16/04 08:23 AM (19 years, 6 months ago)

What I gather from reading this thread is, with the addition of brassinosteroid, colonization will speed up and fruits will be larger, but potency will not go up.

Then, does that mean that although the fruits will be larger, you will have to eat more to have the same effect, since its the same amount of actives, but spread over a larger amount of fruit?




Also, 5 grams of 0.1% pure Brassinolide for $10? That doesn't sound like too good of a deal. How much would you have to add per 1qt of substrate to achieve a noticeable effect?

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OfflineBlue Helix
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Re: hormones gartz used? *brassinosteroid* [Re: FirstAvailable]
    #3142857 - 09/16/04 09:07 AM (19 years, 6 months ago)

I don't know, but the bee pollen itself seemed to work for me. You go through these fancy isolation procedures and buy this expensive stuff, but if the bee pollen works, why bother? At least try the bee pollen before you knock it.

As far as the fruits being weak when they are big, that is a myth. Fruits are weak 99% of the time because of one of two things: (a) one doesn't pick early enough or (b) one don't dry their shrooms well enough. Particularly the drying part is critical and can be very difficult with big fruits if you are just doing passive dessicant drying (without a fan)--poor drying procedure is how this big fruits are weak myth started. Fan blowing through the mushrooms in the open for a day or two followed by a fan blowing through with a dessicant in a closed container is the only way to dry really well. The exception being if you live in a very dry area like a desert in which case you can skip the dessicant.

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Offlinescatmanrav
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Registered: 05/08/04
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Re: hormones gartz used? *brassinosteroid* [Re: Blue Helix]
    #3143316 - 09/16/04 11:30 AM (19 years, 6 months ago)

Ahh makes sense, I always wondered how that rumor got started. It's silly as hell.


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"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay

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OfflineFirstAvailable
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Re: hormones gartz used? *brassinosteroid* [Re: Blue Helix]
    #3143344 - 09/16/04 11:43 AM (19 years, 6 months ago)

I wasn't referring to the rumor. Earlier in this thread, it was stated that due to the additive, the fruits grow larger, but potency does not increase. Is this true?

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InvisibleShmoppy McGillicuddy
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Re: hormones gartz used? *brassinosteroid* [Re: FirstAvailable]
    #3143591 - 09/16/04 01:04 PM (19 years, 6 months ago)

Potency does not increase, but that doesn't mean it decreases. The fruits will still have normal potency, but a better yield.


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OfflineFirstAvailable
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Re: hormones gartz used? *brassinosteroid* [Re: Shmoppy McGillicuddy]
    #3144211 - 09/16/04 03:06 PM (19 years, 6 months ago)

Oh, ok

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InvisibleJoshua
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Registered: 10/27/98
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Re: hormones gartz used? *brassinosteroid* [Re: mycofile]
    #3144362 - 09/16/04 03:38 PM (19 years, 6 months ago)

I used 3% bee pollen granules as a liquid medium. The resultant growth is the most rigerous growth I have seen in a liquid culture. The jars that were inoculated with this culture grew to be some of my best looking cultures.

Joshua


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The Shroomery Bookstore

Great books for inquiring minds!

"Life After Death is Saprophytic!"

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InvisibleShmoppy McGillicuddy
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Re: hormones gartz used? *brassinosteroid* [Re: Joshua]
    #3144495 - 09/16/04 04:05 PM (19 years, 6 months ago)

3% by weight? or volume?

I have a bunch of bee pollen sitting around. SWISM tried it as an additive to WBS, but had problems with carmelization of the sugars in the pollen.


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OfflineGr0wer
always improving
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Re: hormones gartz used? *brassinosteroid* [Re: Shmoppy McGillicuddy]
    #3144557 - 09/16/04 04:25 PM (19 years, 6 months ago)

Sounds like we have a new TEK. Was the mix only 3% pollen or did it have any other additives? Where can one get bee pollen?

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InvisibleJoshua
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Re: hormones gartz used? *brassinosteroid* [Re: Shmoppy McGillicuddy]
    #3144843 - 09/16/04 05:52 PM (19 years, 6 months ago)

3% by weight. I may show some pics of the culture.

If you want to incorporate bee pollen in grain jars, I would adjust your method. I wouldn't PC the pollen solution for longer than 15 minutes. Perhaps you could cook the grain while a bit dry and add the 15 min bee pollen solution with the spores such as in a syringe after the cooking of the grain.

Joshua


--------------------
The Shroomery Bookstore

Great books for inquiring minds!

"Life After Death is Saprophytic!"

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OfflineSpudz76
Got mycelium on the brain...
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Registered: 02/08/04
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Re: hormones gartz used? *brassinosteroid* [Re: Joshua]
    #3145022 - 09/16/04 06:45 PM (19 years, 6 months ago)

That method would seem to make sense, good idea!

I found about a bajillion places to get it from, but this place has it for $18.18 per pound which seems like plenty for a long while. But it does seem to have a relatively short shelf life and should be refrigerated - I'm sure there are places to get lesser amounts. Also, being a human dietary supplement you ought to be able to find some at the local health nut store or maybe even GNC, but maybe not as pure (like, buffered for digestion or to increase shelf life, which probably won't help the mycelium digest it any better).


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Anywhere in this posting where I have referred to myself or my possessions, it is a personalization of events and items experienced or possessed by someone else.  I do not personally do anything illegal, and I hope nobody else does either.

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OfflineBlue Helix
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Re: hormones gartz used? *brassinosteroid* [Re: Spudz76]
    #3146646 - 09/17/04 12:41 AM (19 years, 6 months ago)

You can find bee pollen on Ebay too. I just did a search and found 100% pure bee pollen for $9.50 on Buy It Now plus $4.50 shipping. I have used bee pollen in manure, mixing it prior to pasturization at a concentration MUCH lower than 3%. The colonization was very aggressive and the fastest I've seen.

Edited by Blue Helix (09/17/04 12:42 AM)

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OfflineBlue Helix
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Re: hormones gartz used? *brassinosteroid* [Re: Blue Helix]
    #3146755 - 09/17/04 01:11 AM (19 years, 6 months ago)

Oh, and just for the record, when using the bee pollen in a manure/vermiculite/perlite/coir blend total bed colonized happened in 6 days using ordinary spawn ratios. Typically bed colonization takes me closer to 10 days.

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OfflineSpudz76
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Re: hormones gartz used? *brassinosteroid* [Re: Blue Helix]
    #3147280 - 09/17/04 07:02 AM (19 years, 6 months ago)

Wow, I wasn't picturing near twice the speed. How about pinning speed later on?


--------------------
Anywhere in this posting where I have referred to myself or my possessions, it is a personalization of events and items experienced or possessed by someone else.  I do not personally do anything illegal, and I hope nobody else does either.

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OfflineBlue Helix
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Re: hormones gartz used? *brassinosteroid* [Re: Spudz76]
    #3147537 - 09/17/04 08:53 AM (19 years, 6 months ago)

I should know in about two weeks how that particular tray fruits...  :wink:

Edited by Blue Helix (09/17/04 08:53 AM)

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OfflineBlue Helix
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Re: hormones gartz used? *brassinosteroid* [Re: Blue Helix]
    #3147558 - 09/17/04 09:00 AM (19 years, 6 months ago)

By the way, a lot of things account for colonization speed, including the particular strain that pops up (it was from a multispore culture, not a clone). I cannot reasonably say the average increase in speed is roughly double until I have more data than one tray. I can say, though, that this tray that I used the bee pollen has colonized faster than any other I have seen and the increase in speed was significant, well outside the range of a random fluke I believe.

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Shop: Mushroom-Hut Liquid Cultures   Original Sensible Seeds Autoflowering Cannabis Seeds   PhytoExtractum Buy Bali Kratom Powder   Left Coast Kratom Buy Kratom Extract   Bridgetown Botanicals Bridgetown Botanicals   North Spore Bulk Substrate   MagicBag.co Certified Organic All-In-One Grow Bags   Kraken Kratom Kratom Capsules for Sale   Myyco.com Golden Teacher Liquid Culture For Sale


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