Home | Community | Message Board


Vaposhop
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop for: ½ Pint Jars, Wild Bird Seed

Jump to first unread post. Pages: 1
OfflineSatori1
Stranger

Registered: 12/05/03
Posts: 162
Loc: everywhere
Last seen: 10 years, 11 months
Grain to grain transfer bags not fully colonizing
    #3055798 - 08/26/04 11:45 PM (12 years, 3 months ago)

This is a strage problem, and I can't figure out for the life of me why it's happening. Someone created WBS bags and transfered colonized grain from another bag as a g2g transfer.
The G2 bags colonized a little for the first few days, then quit. What could possibly cause this? lack of air exchange mabey?

The master grain and G2 grain were incubated in two different incubators,with the same basic set-up - maintaining a temperature range of 83-86 F. The incubator which the G2 grain was placed had the plastic tote cover on it, with a blanket draped over it. The incubator which successfully incubated the master spawn did NOT have the plastic cover on it, only a few thick blankets draped over the opening. I don't if this would make any kind of difference.

There are no signs of contamination of any sort, it's been ~12 days since the G2G transfer took place. The master grain was inoculated via spore syringe. In both instances the bird seed was prepared in the same way. The only difference was that they were incubated in different incubators, with the g2 grain incubator being a few liter's less volume than the master spawn incubator, and the tote cover.

So, knowing all this, what could possibly make the G2 grain stall like this?


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibledog
straw dog

Registered: 06/20/02
Posts: 2,790
Loc: Route 66
Re: Grain to grain transfer bags not fully colonizing [Re: Satori1]
    #3055970 - 08/27/04 12:32 AM (12 years, 3 months ago)

Bacterial contaminations are often invisible.


--------------------


Fascism (fash'izem) n. A governmental system marked by a centralized dictatorship, stringent socioeconomic controls, and often belligerent nationalism. see also: the Bush Administration.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineSatori1
Stranger

Registered: 12/05/03
Posts: 162
Loc: everywhere
Last seen: 10 years, 11 months
Re: Grain to grain transfer bags not fully colonizing [Re: Satori1]
    #3057662 - 08/27/04 12:44 PM (12 years, 3 months ago)

.. I don't think contamination is the problem, but it's looking that way


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleTomandjerry58
Stranger
Registered: 01/27/03
Posts: 5,209
Re: Grain to grain transfer bags not fully colonizing [Re: Satori1]
    #3057795 - 08/27/04 01:27 PM (12 years, 3 months ago)

i think you might have answered ur own question with the lack of air. have u ever used quart jars?with 3/8 inch hole drilled in top with synthetic filter discs. if not try it. usually two spoon fulls and a good shake will colonize a jar in 8 days.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflinepsilocyberV
old hand

Registered: 06/09/99
Posts: 1,809
Last seen: 29 days, 5 hours
Re: Grain to grain transfer bags not fully colonizing [Re: Satori1]
    #3058085 - 08/27/04 02:32 PM (12 years, 3 months ago)

Most invisible contaminants will emit a very distinct odor from the filter patch on the bag. They will smell very "sour" sometimes strongly like ammonia if it is something like wet spot bacteria.

Stalling in bags that are otherwise sealed and spawned properly is almost always due to water ratios being off. How was the grain prepared to get the water content correct? Have you tried shaking and mixing the colonized grains with the uncolonized grains inside the bag? Make sure your filter patches are not restricted or being blocked by something in the incubator.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineSatori1
Stranger

Registered: 12/05/03
Posts: 162
Loc: everywhere
Last seen: 10 years, 11 months
Re: Grain to grain transfer bags not fully colonizing [Re: psilocyber]
    #3058975 - 08/27/04 06:34 PM (12 years, 3 months ago)

There is no sour smell, and I am familiar with the smells of invisible bacteria, such as wet rot. There are no unusual smells of any kind. The filter patches were not blocked or in any way restricted from breathing.

The master grain and G2 grain were prepared in the same way -
soak for ~12 hours, skimmed floaties off, rinsed, drained for 30 min, pc'ed for 90 min and innoculated.

Initially, the colonized grain was mixed evenly throughout the uncolonized kernels, then left for a few days, during which time growth appeared to be normal. then after ~3 days it just stopped. Yesterday it was mixed again, with no signs of recovery.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineSexySmurff
HyperQube
Male User Gallery

Registered: 06/21/04
Posts: 507
Loc: HyperSpace
Last seen: 8 days, 11 hours
Re: Grain to grain transfer bags not fully colonizing [Re: Satori1]
    #3059943 - 08/27/04 11:08 PM (12 years, 3 months ago)

contaminated...sorry.


--------------------
Exalted is He from what they utter. Exalted is He from what they imagine. Exalted is He that exists without a place. Exalted is He that was there forever without a time or a beginning. Exalted is He that will be there forever without a time or an end. Exalted is He that will forever be praised. That is your God.


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop for: ½ Pint Jars, Wild Bird Seed

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* Is this bag fully colonized? buttmonkey24 1,175 10 03/29/09 02:49 PM
by anakin3197
* ~Should I Wait for 100% Colonization For Grain Transfer....~??? crazyoutlaw 1,254 18 11/16/09 02:02 PM
by PassiveAgressive
* Fully colonized WBS smells funny Jrsxt 947 7 06/19/08 12:37 PM
by Jrsxt
* Grain 2 Grain Q?
( 1 2 all )
Choking victim 1,172 23 12/30/08 09:51 AM
by Choking victim
* Quicki how long will take my 3lb to fully colonize TheMan420 1,693 18 08/16/07 07:31 PM
by TheMan420
* Time estimate from Grain transfer to Fruiting in Growbag KauaiOrca 490 1 08/25/08 05:38 PM
by hiredman
* Grain to grain transfers = Uber! Pr0_X 498 2 11/29/07 11:32 AM
by richismalls
* does the jar have to be fully colonized in GTG transfers? arp180 537 5 11/02/08 07:17 PM
by arp180

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Magash, Shroomism, george castanza, Prisoner#1, RogerRabbit, Citric, total, FooMan, 13shrooms, stonesun, EvilMushroom666, cronicr, PussyFart
924 topic views. 33 members, 92 guests and 29 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Edabea
Please support our sponsors.

Copyright 1997-2016 Mind Media. Some rights reserved.

Generated in 0.035 seconds spending 0.003 seconds on 14 queries.