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OfflineTriple_3
member
Registered: 03/21/01
Posts: 99
Last seen: 22 years, 8 months
Re: liquid innoculation
    #305418 - 04/30/01 09:09 PM (22 years, 9 months ago)

yes exactly works better than honey water
i think that the agar helps it germinate faster and stronger
it also works with a stem

4%malt to water is supposed to work

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OfflineSuntzu
Geek
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Registered: 10/14/99
Posts: 1,396
Last seen: 4 months, 4 days
Re: liquid innoculation
    #306329 - 04/30/01 10:55 PM (22 years, 9 months ago)

Agarical; thanks for the credit, but I think I remember reading about that here, don't remember who. A long long time ago. I don't know, maybe not. Could have been a dream.
[stoner]

Have you ever left your blended solutions sitting around [covered] for a week or so? Mine form a surface layer of new mycelial growth, I think the agar chunks float to the surface giving the fungus more surface tension and nutrients, so it's like a very wet agar plate. Looks funny, though, a 1/2 pt of water with fresh mycelium covering the surface.


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OfflineAzure
old hand
Registered: 12/31/98
Posts: 469
Loc: California, USA
Last seen: 21 years, 9 months
Re: liquid innoculation
    #306345 - 04/30/01 11:13 PM (22 years, 9 months ago)

Agarical-How did you make the home made blender base?


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OfflineBleuboxo
enthusiast
Registered: 04/05/01
Posts: 196
Loc: Geographic Location (Stat...
Last seen: 22 years, 7 months
Re: liquid innoculation
    #310748 - 05/06/01 05:53 PM (22 years, 9 months ago)

this is what i usually do. no blenders they are not good for mycelia in my opinion. others have there opinions and results though.
take 4% malt extract and mix with water. cook for 45minutes. let cool down and put a whole agar dish in, you can cut it up into fourths if you want and put one fourth in there but i usually put the whole things and it gets loaded with mycelia in about 2 weeks. shake every day. if you dont want all the liquid you can put some grain in there once its colonized and shake it up. wait a couple days and drain innoculant out from the small hole on the lid. leave rye in there for two days and use that to spawn other substrates.

" Insanity is just a thread of reality...the make-as-you-go part of living, the bare second reflex of dying _Stavros Christou... by_"


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" Insanity is just a thread of reality...the make-as-you-go part of living, the bare second reflex of dying _Stavros Christou... by_"

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OfflineAzure
old hand
Registered: 12/31/98
Posts: 469
Loc: California, USA
Last seen: 21 years, 9 months
Re: liquid innoculation [Re: Bleuboxo]
    #311513 - 05/07/01 04:41 PM (22 years, 9 months ago)

That kind of defeats the purpose of getting an even distribution of mycelium in jars. It'd be easier to just place the agar wedge directly onto grain to save time.
What I've noticed is that you definitely need to make a very dilute solution of malt. The more you put in the water, the harder it is to break it up and suck into the syringe. The nail/sharp object idea sounds excellent, but you need to make sure you get stainless steel or a metal that doesn't ionize in water.


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OfflineAgarical
journeyman

Registered: 01/14/01
Posts: 21
Last seen: 22 years, 25 days
Re: liquid innoculation [Re: Azure]
    #312656 - 05/08/01 08:20 PM (22 years, 9 months ago)

Azure, the blender base isn't home made. I just remove the original glass blender container, and replace it by threading a jar into the blender base. Take a look at your blender, and you'll figure it out. Most blenders are usually 3 pieces. The base (motor), the blades (with gasket and threaded insert) and the glass container with lid.


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