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Offlineshallow
member
Registered: 07/04/04
Posts: 229
Last seen: 15 years, 11 months
Airflow in jars
    #3049281 - 08/25/04 04:19 PM (19 years, 7 months ago)

Ive got a few jars going ATM. Ive read on the boards from people I respect that you should keep you jars covered and that you shouldnt?
So...ugh...whats the consensus? Should I peel the tape or leave it on?

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OfflineMycoGlowFlow
between the walls
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Registered: 05/20/01
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Re: Airflow in jars [Re: shallow]
    #3049294 - 08/25/04 04:22 PM (19 years, 7 months ago)

leave the tape on and untighten the lid a little bit, that way there is still a shield from falling contams, also keep ur jars in a very clean place.. some ppl say that unscrewing lids or peeling off injection hole tape is too risky but the fact of the matter is that if there is a lack of O2 the growth will slow down to point where the contams when the grow race


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(the key-strokes that have completed everything ever typed on this user name have been a random coincidence, except for the words in this notation)

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OfflineMad_Hatter2004
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Registered: 06/19/04
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Re: Airflow in jars [Re: MycoGlowFlow]
    #3049315 - 08/25/04 04:27 PM (19 years, 7 months ago)

Leave the tape on until you see signs of mycelium,then peel back the tape.The dry vermiculite layer (assuming you're using PF Tek) on top of the substrate is a barrier for contaminates (or so I understand and have been told).


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Offlinehyphae
born to grow
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Registered: 12/13/02
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Re: Airflow in jars [Re: shallow]
    #3049372 - 08/25/04 04:41 PM (19 years, 7 months ago)

Leave tape off holes after innoculating. It's easier to do it then than later IME, Of course it's not necessary to take tape off until myc starts growing but forgeting to take it off is too easy.Bottom line take the tape off it makes for a healthier culture all around :wink:

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Offlinemeanoldman
old guy
Registered: 06/14/04
Posts: 96
Last seen: 3 years, 9 months
Re: Airflow in jars [Re: shallow]
    #3049387 - 08/25/04 04:46 PM (19 years, 7 months ago)

Next time, don't bother taping the holes. Just use a ~6" square piece of aluminum foil and cover the top of the jar like a form fitted umbrella during the PC-ing. Leave it on until innoculation, it keeps the top of the lid sterile until innoc time. Much quicker too. After innoc, they need no tape or foil, the dry verm layer does it all.

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Offlinezappaisgod
horrid asshole

Registered: 02/11/04
Posts: 81,741
Loc: Fractallife's gym
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Re: Airflow in jars [Re: shallow]
    #3049391 - 08/25/04 04:47 PM (19 years, 7 months ago)

What about polyfill stuffed tightly in a hole in the center of the lid?


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Offlinemeanoldman
old guy
Registered: 06/14/04
Posts: 96
Last seen: 3 years, 9 months
Re: Airflow in jars [Re: shallow]
    #3049405 - 08/25/04 04:50 PM (19 years, 7 months ago)

Air exchange isn't a problem with PF style jars as they colonize, just leave the lid a little loose. No need for polyfill or anything.

(I assumed this guy is doing PF style jars)

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Offlinehyphae
born to grow
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Registered: 12/13/02
Posts: 6,228
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Re: Airflow in jars [Re: meanoldman]
    #3049564 - 08/25/04 05:21 PM (19 years, 7 months ago)

Quote:

meanoldman said:
Air exchange isn't a problem with PF style jars as they colonize, just leave the lid a little loose. No need for polyfill or anything.

(I assumed this guy is doing PF style jars)



Unless you leave the tape on the holes and jars sealed tight :wink: Like meanoldman said crack the lids a bit thats all ya need to do.

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OfflineWorf
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Registered: 07/04/04
Posts: 15,663
Loc: Final Frontier
Last seen: 11 years, 1 month
Re: Airflow in jars [Re: shallow]
    #3049623 - 08/25/04 05:33 PM (19 years, 7 months ago)

you can take the tape off because the top layer of verm protects it from contams. Just be sure not to disturb the jar so the vermiculite doesn't move

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