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Amazon Shop for: ½ Pint Jars, Brown Rice Flour, Vermiculite

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Offlinediscman1
journeyman
Registered: 08/24/04
Posts: 962
Last seen: 11 years, 11 months
8 days, no signs of growth..
    #3044829 - 08/24/04 05:25 PM (12 years, 3 months ago)

I realize it could take up to 2 weeks, but I was really hoping for 2-5 days. :P Should I be concerned at all? I'm using 1/2 pint jars. 2 innoculation points, using organic brown rice flour and vermiculite. I sterilized for an hour at 20PSI just for added security. :wink: I let the jars cool for 24+ hours before innoculation.

Nothing else seems to be growing either, so at least I know the jars are sterile.. I guess.

The Puerto Rican spores that I used were from 12/03, so almost a year old. :frown: They also weren't stored in the best conditions(out of light, but rather high temperatures.. 80 - 100?F). I am hoping that isn't my problem, but somehow I think it could be.

In the future, should I store syringes in the fridge?

Thanks for you replies. :smile:


Edited by discman1 (08/24/04 06:00 PM)


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Invisiblemyndreach
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Registered: 08/07/04
Posts: 2,368
Re: 8 days, no signs of growth.. [Re: discman1]
    #3044959 - 08/24/04 05:54 PM (12 years, 3 months ago)

welcome to the boards friend.

I'm not sure about syringe storing...sorry. But give it time...a couple to few days isn't much. Give it at least a week before you worry :smile:


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InvisibleFucknuckle
Dog Lover

Registered: 04/24/04
Posts: 6,762
Re: 8 days, no signs of growth.. [Re: discman1]
    #3044984 - 08/24/04 06:02 PM (12 years, 3 months ago)

Your spores are dry from all the sitting around and high temps you stated.

They are going to need 2-3 days to rehidrate then a couple more to germinate.
So I would guess you should see some growth in the next 2-3 days.

Question? Do you have them incubated at 84-86 degs.???

That will help them move along %200-300 faster.


--------------------
What it is, is what it is my Brother.
It is as it is, so suffer thru it.


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Offlineskullfarmer1979
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Registered: 05/02/04
Posts: 506
Loc: Bum Fuck, Egypt
Last seen: 11 years, 9 months
Re: 8 days, no signs of growth.. [Re: discman1]
    #3045195 - 08/24/04 06:55 PM (12 years, 3 months ago)

i'm thinking your spores are no good dude.if i was you,i would innoculate them again using more spore water this time.i bought some new spore syringes once and i had the same problem .i ended up using 1 syringe to innoculate 3 fucking jars! peace


--------------------
I TASTE THE WREAKAGE OF CRUMBLING FACES,I KNOW THE PALE THING IN THE DARKEST OF PLACES. -DAX RIGGS-

get the most out of life,listen to ACID BATH!!!!


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Offlinediscman1
journeyman
Registered: 08/24/04
Posts: 962
Last seen: 11 years, 11 months
Re: 8 days, no signs of growth.. [Re: Fucknuckle]
    #3045211 - 08/24/04 06:58 PM (12 years, 3 months ago)

I forgot to mention that it was a spore syringe, not a print.. so I don't think the hydration thing applies, could be wrong, though.

Yes, temperatures have been betwen 78 and 86?F the whole time, as per the outdoor thermometer probe that is in the box at the same level the jars are.

Does it absolutely have to be 100% dark? There is probably a little light leak, since I use a small 15W fluorescent light to keep the shelf warm(It doesen't shine on the jars directly, but there is probably a little light leak in the cardboard box they're in).

Yeah, I've thought about dumping the remaining spore solution I have into the jars, but I figured at the least I should try some new ones, just incase it was something other than the spores.

I used about 6cc between 7 jars..

Honestly, the syringe didn't look very populated with spores(although you could certainly see them, and they weren't clumpy), at least compared to some of the syringe pictures I've seen where they're like actually purple-hued.


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Offlineskullfarmer1979
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Registered: 05/02/04
Posts: 506
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Last seen: 11 years, 9 months
Re: 8 days, no signs of growth.. [Re: discman1]
    #3045402 - 08/24/04 07:39 PM (12 years, 3 months ago)

i would keep my jars in constant light if i was you.they will colonize faster with 24hour light. you kind of sound like a newbie,search around the site some more and you will find all you need.peace


--------------------
I TASTE THE WREAKAGE OF CRUMBLING FACES,I KNOW THE PALE THING IN THE DARKEST OF PLACES. -DAX RIGGS-

get the most out of life,listen to ACID BATH!!!!


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Offlineliveby
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Registered: 06/15/04
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Re: 8 days, no signs of growth.. [Re: discman1]
    #3045418 - 08/24/04 07:44 PM (12 years, 3 months ago)

same here , 10 days no growth im not worried!


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Offlinediscman1
journeyman
Registered: 08/24/04
Posts: 962
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Re: 8 days, no signs of growth.. [Re: skullfarmer1979]
    #3045529 - 08/24/04 08:01 PM (12 years, 3 months ago)

I am a newbie, but I have done lots and lots of reading. I have a green thumb, so most of the concepts aren't that foreign to me.

I thought mycelium was supposed to grow in the dark and that light was only needed to induce fruiting?

Those are its normal natural conditions, as far as I know.


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Offlineliveby
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Re: 8 days, no signs of growth.. [Re: discman1]
    #3045547 - 08/24/04 08:03 PM (12 years, 3 months ago)

no coldshocking is used to introduce pinning , light itself is only needed at the pinning/fruiting stage,P.j


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OfflineUncleMike
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Re: 8 days, no signs of growth.. [Re: discman1]
    #3045580 - 08/24/04 08:10 PM (12 years, 3 months ago)

There are a lot of reasons that could be the reason for the slow growth. 1 strand you are using. B+ and Malaysian are very slow. The light really won't retard the growth. the light can cause premature pinning in the jar. the spores are old so that can slow down things. give it a while longer. Also you may have growth and don't realize it.


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Live each day like it will be your last, tomorrow my never come.
SporeSmart


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Amazon Shop for: ½ Pint Jars, Brown Rice Flour, Vermiculite

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