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Amazon Shop for: ½ Pint Jars, Agar, Microscope, Pressure Cooker, Rye Grain, Vermiculite

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Offlinecarpediem13
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Registered: 08/17/04
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Rye Jars
    #3035731 - 08/22/04 10:48 AM (12 years, 7 months ago)

Just a few questions I couldn't piece together from all the teks I've been reading...

Ok... say there are sterile rye jars made with a pressure cooker. Is it a really bad idea to let them sit a week or two before inoculation? I know it would be better to do it quicker... but does it have to be done right away or is it possible to make them wait some? Also, everytime I see a tek for this it says "shoot 2-3 cc of syringe into the jars." Well, in the pf tek you have to push the needle through the verm and against the glass, but in the rye teks it doesn't even look like the needle will reach the rye. Do I just shoot it on top of the rye? Last question... since casings are moist, do they have to be put in a terrarium for humidity or can they be left out on their own?

Thanks in advance! Later


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Life is a dream on the way to death...


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OfflineJaRRn
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Re: Rye Jars [Re: carpediem13]
    #3035736 - 08/22/04 10:49 AM (12 years, 7 months ago)

>Do I just shoot it on top of the rye

You shake the Jar  :twirlyface:


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Offlinecarpediem13
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Registered: 08/17/04
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Re: Rye Jars [Re: JaRRn]
    #3035813 - 08/22/04 11:31 AM (12 years, 7 months ago)

So I just shoot 2-3 cc on top of the grain and then shake the jar?


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Life is a dream on the way to death...


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OfflineRogerRabbitM
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Re: Rye Jars [Re: carpediem13]
    #3035842 - 08/22/04 11:49 AM (12 years, 7 months ago)

I don't shake rye jars until about 30% colonization. Shoot the spore solution down the edge of the glass so it runs as far down the jar as possible, not into the middle of the rye. Don't shake right away. Wait for germination and partial colonization for best results.

Jars should be used as soon as they are sterilized. Some people have waited longer and still succeeded, but it's rare. Use them right away, or re-sterilize.


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OfflineAnnoA
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Re: Rye Jars [Re: RogerRabbit]
    #3035945 - 08/22/04 12:40 PM (12 years, 7 months ago)

I never had problems with shaking it right away, actually I get a faster colonisation this way.


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Offlinedoug_E_fresh
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Registered: 07/01/04
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Re: Rye Jars [Re: Anno]
    #3036432 - 08/22/04 03:58 PM (12 years, 7 months ago)

Quote:

Anno said:
I never had problems with shaking it right away, actually I get a faster colonisation this way.




Indeed, same with me.

Anyway
>Also, every time I see a tek for this it says "shoot 2-3 cc of syringe into the jars." Well, in the pf tek you have to push the needle through the verm and against the glass, but in the rye teks it doesn't even look like the needle will reach the rye. Do I just shoot it on top of the rye?

Just stick the needle in the jar hole, shoot the spores in there, cover the hole you just put into the lid with tape or a band aid, then....SHAKE LIKE CRAZY.


>Last question... since casings are moist, do they have to be put in a terrarium for humidity or can they be left out on their own?

Kind of confusing question (at least I think). But let?s see if I get it right. When you shoot up the jars, if you made an incubator, then put them in there. If not (in my case I don?t use an incubator), put them in a dark place. Either way, I shake at around 50-80% colonized. Then let the mycelium rebuild, and you?re ready to case.

Anyway, check this out, this is magash's grain tek and his martha setup. I use both. http://www.shroomery.org/forums/showflat...;o=&fpart=1


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Edited by doug_E_fresh (08/22/04 04:04 PM)


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Offlinehyphae
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Re: Rye Jars [Re: carpediem13]
    #3036499 - 08/22/04 04:28 PM (12 years, 7 months ago)

It is always advantagous to shake right away especially when doing G2G transfers. Shooting in the middle insures all spores are on grain and not stuck to the glass which is really no biggie as germination will still take place bigtime. People need to realize the more initial contact the faster the results (basic) this is a microscopic environment, one piece of of myc covered agar can leave microscopic "bits" throughout an entire jar if shaken "like a crazy madman" or shook and rolled, shook and rolled repeatedly. then another shake will serve two purposes, one it will distribute colonized grains even more throughly and as important will show any jars that may be contamed (they will show slowed growth). When to shake is IMHO a personal preference, anywhere from 30-50% is beneficial after that you really missed the benefits of shaking (fact) GL guys


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Offlinescatmanrav
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Re: Rye Jars [Re: hyphae]
    #3037482 - 08/22/04 09:46 PM (12 years, 6 months ago)

When casing youll need to put them in a terrerium. Usually room rh will dry out casings. Need I think at least 85% rh but I keep at ~99%.


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"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


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Amazon Shop for: ½ Pint Jars, Agar, Microscope, Pressure Cooker, Rye Grain, Vermiculite

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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