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OfflineKatrina

Registered: 08/18/04
Posts: 131
Last seen: 9 months, 20 days
first jars--probably mold??? pics included
    #3020625 - 08/18/04 11:32 PM (17 years, 2 months ago)

My first ever jars. I did 12 PF tek. Started with one jar growing little green. I took it out, then got 2 more. I took those out. Came back from a trip 3 days later and now I have 4 more with green. I've taken them all out. I am pretty sure these are mold??? What did I do wrong. How can I not do this next time. Are my last 5 jars going to get infected too? Please help






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OfflineMycofriend
Stranger
Registered: 10/23/01
Posts: 25
Last seen: 3 years, 7 months
Re: first jars--probably mold??? pics included [Re: Katrina]
    #3020695 - 08/18/04 11:51 PM (17 years, 2 months ago)

You are gonna have to explain your whole procedure you used. Once that is explained, then people can give you advice.


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OfflineWorf
Lt. Commander

Registered: 07/04/04
Posts: 15,663
Loc: Final Frontier
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Re: first jars--probably mold??? pics included [Re: Katrina]
    #3020716 - 08/18/04 11:57 PM (17 years, 2 months ago)

thats some science experiment you have going there.

Doesn't PF tek recommend half-pint jars?

You might try taken them out and then just carefully take it apart to see where the contams came from. If it's all over it might not have been PC'd well enough.


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OfflineKatrina

Registered: 08/18/04
Posts: 131
Last seen: 9 months, 20 days
Re: first jars--probably mold??? pics included [Re: Mycofriend]
    #3020720 - 08/18/04 11:58 PM (17 years, 2 months ago)

OK. Sorry that would probably help. I did the PF tek as far as substrate mix. I used a pressure cooker 15 psi for 2 hours just to be extremely safe. I kept the holes taped at first and then I read on here not to do that, so I untaped them. The only thing I can think of was the tek said to sterilize the syringe only if it got touched. Now I wonder if should sterilize after each jar anyway or after each hole?? I also used rainwater as someone said to use organic water, but I don't think that would do anything as it was PC'd for 2 hours. I am really worried about my remaining 5 jars. I just dumped the others and cut through them and the green stuff seemed to go through to the center in some, only on the outside in others. Mold did seem to line up with my inoculation holes. I've kept them in a rubbermaid container at about 70 to 75 degrees. Seems like I got good white stuff, but then this stupid green stuff!!! I had 2 diff strains, EC and Orissa. I have more EC green, only have 2 left of it. Just need to know what I did wrong I guess.


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OfflineKatrina

Registered: 08/18/04
Posts: 131
Last seen: 9 months, 20 days
Re: first jars--probably mold??? pics included [Re: Katrina]
    #3020728 - 08/18/04 11:59 PM (17 years, 2 months ago)

They are 1/2 pint jars. Pic must make them look bigger.


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OfflineRat
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Registered: 04/20/04
Posts: 103
Last seen: 16 years, 2 months
Re: first jars--probably mold??? pics included [Re: Katrina]
    #3020834 - 08/19/04 12:15 AM (17 years, 2 months ago)

Did you have foil or any type of filter over your lids? If not, use tyvek, coffee filters, and foil just to be really safe.


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OfflineKatrina

Registered: 08/18/04
Posts: 131
Last seen: 9 months, 20 days
Re: first jars--probably mold??? pics included [Re: Rat]
    #3020879 - 08/19/04 12:27 AM (17 years, 2 months ago)

I used foil over the lids while they were in the pressure cooker. Is that right??


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Offlineletstakeapeak
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Registered: 07/30/04
Posts: 7
Last seen: 17 years, 1 month
Re: first jars--probably mold??? pics included [Re: Katrina]
    #3021325 - 08/19/04 01:38 AM (17 years, 2 months ago)

Theres no way those are half pint jars.The mouths of the jars are much wider.Half pint jars are much smaller check the box they came in.


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Offlineletstakeapeak
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Registered: 07/30/04
Posts: 7
Last seen: 17 years, 1 month
Re: first jars--probably mold??? pics included [Re: Katrina]
    #3021335 - 08/19/04 01:40 AM (17 years, 2 months ago)

Where did You get your spores from


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OfflineKatrina

Registered: 08/18/04
Posts: 131
Last seen: 9 months, 20 days
Re: first jars--probably mold??? pics included [Re: letstakeapeak]
    #3022568 - 08/19/04 11:04 AM (17 years, 2 months ago)

Yes they are 1/2 pint jars, box says and the mixture stated in the PF tek fit perfectly. I mixed each one individually. I got spores from spore101. Could someone please help me with my real problem?


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OfflineAnnoA
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Registered: 06/18/99
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Re: first jars--probably mold??? pics included [Re: Katrina]
    #3023638 - 08/19/04 03:22 PM (17 years, 2 months ago)

It looks like the syringes were contaminated since the contamination starts deep down on the inoculation points. Happens.
I would ask for replacement syringes.


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Offlinecaimen
the unknown
Registered: 06/22/04
Posts: 184
Loc: HERE
Last seen: 15 years, 11 months
Re: first jars--probably mold??? pics included [Re: Katrina]
    #3036061 - 08/22/04 01:33 PM (17 years, 2 months ago)

Hey im kinda new at this too so i know where u are coming from . I had some trouble with contams at frist. You said u flamed the needle once? I flamed my needle every 2 holes and wipe with 70% before every hole. make your self a Alchol lamp Link is at the bottom of my post. very helpfull IMO. I thought my syringes where bad too but i made a karo/water jar and had no contams. save your last 2ccs for a karo/water jar and go from there. PCing for 2 hours is a long time.

CLEAN is very very imporantant.


--------------------
MAN Made Speed
GOD Made Weed
<Who do YOU TRUST ? >

http://www.shroomery.org/index/par/25954


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Offlineasporeprint
Stranger
Registered: 06/04/04
Posts: 10
Last seen: 16 years, 10 months
Re: first jars--probably mold??? pics included [Re: Katrina]
    #3036126 - 08/22/04 01:51 PM (17 years, 2 months ago)

those are 1/2pint jelly jars there not the right ones try again dont pc for so long it carmelizes the grain an wont let the spores grow out


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