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OfflineToricious
Theblunt-smokinglense-man.

Registered: 09/27/03
Posts: 688
Last seen: 12 years, 6 months
fruitbody question
    #3020611 - 08/18/04 11:29 PM (13 years, 7 months ago)

Hi, ive been wondering about something lately - in paul stamets mushroom cultivator it states that you can only take a fruitbody isolate within 48 hours of its being picked - it also says that mushrooms are really compressed mycilium, and on the part where it talks about slants it talks about conserving the specimen by lowering the temperature to 2 to 4 degrees to stop the growth. Logically speaking if you were to refrigerate a fruitbody to the right temperature 48 hours after picking wouldnt the mushroom be viable for cloning forever? Couldnt it be like having a slant if you were just to keep a mushroom in the fridge instead?


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InvisibleFucknuckle
Dog Lover

Registered: 04/24/04
Posts: 6,762
Re: fruitbody question [Re: Toricious]
    #3020734 - 08/19/04 12:00 AM (13 years, 7 months ago)

No It would ROT after a few weeks

Myc isolated in a slant is sterile

A mushroom is covered in micro containms and would ROT


--------------------
What it is, is what it is my Brother.
It is as it is, so suffer thru it.


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OfflineToricious
Theblunt-smokinglense-man.

Registered: 09/27/03
Posts: 688
Last seen: 12 years, 6 months
Re: fruitbody question [Re: Fucknuckle]
    #3022480 - 08/19/04 10:23 AM (13 years, 7 months ago)

alright - but before it rots tho could you use the fruitbody for isolates if its been more than 48 hours but under 48 hours since it was slowed down?


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InvisibleFucknuckle
Dog Lover

Registered: 04/24/04
Posts: 6,762
Re: fruitbody question [Re: Toricious]
    #3023722 - 08/19/04 03:43 PM (13 years, 7 months ago)

Yes ther eis a tek here in the tex section that shows exactly how to do just that
Bascily you cut them stem open in a glove box, in front of a hepa air stream, etc. somewhere where the air is sterial

And take your clone tissue from the center
put in a blender adn some sterial water
Blend
SUck into syringes and inculate as usual
Or do a tissue transfer to agar.
Etc...


--------------------
What it is, is what it is my Brother.
It is as it is, so suffer thru it.


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