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Anonymous

Simple question regarding B+ strain on WBS.
    #2997391 - 08/12/04 09:42 PM (12 years, 8 months ago)

Lets play pretend, and say that I inoculated two WBS jars with a B+ syringe. The jars were prepared properly, and have been incubating in the dark at a temperature of 84 degrees for the last 7 days, yet they show no signs of growth. If this were to happen, should I be worried that these jars have failed, or is the B+ strain just a slow starter?


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OfflineShaqAttack
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Re: Simple question regarding B+ strain on WBS. [Re: ]
    #2997449 - 08/12/04 09:57 PM (12 years, 8 months ago)

I'm no expert but there should probably be some sign of life after 7 days.

Did you let the jars cool?

Do you have a hole and filter in the top of the jar for some air/gas exchage?

Maybe drop the temp to 80 degrees?


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Offlineiluan
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Re: Simple question regarding B+ strain on WBS. [Re: ShaqAttack]
    #2997491 - 08/12/04 10:11 PM (12 years, 8 months ago)

84 is fine, you shoulda seen something by now, but if no contams i would let em go a few more days anyway just to be sure, if you pushing 2 weeks w/o growth you might wana worry.


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Anonymous

Re: Simple question regarding B+ strain on WBS. [Re: iluan]
    #2997538 - 08/12/04 10:24 PM (12 years, 8 months ago)

Thanks for the input on both parts, i'll keep an eye on it and just be a bit more patient.


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OfflineJaRRn
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Re: Simple question regarding B+ strain on WBS. [Re: ]
    #2997614 - 08/12/04 10:41 PM (12 years, 8 months ago)

I had made 3 jars of WBS, 2 took off like lightning, the other in a different type of jar, has done SFA after 3 weeks, little bits here and there. dont ask what happened all the WBS was prepared from the same batch.


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OfflineQandA
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Re: Simple question regarding B+ strain on WBS. [Re: JaRRn]
    #2997681 - 08/12/04 11:01 PM (12 years, 8 months ago)

WBS can be funny sometimes. If the water content is too high then that can slow things down drastically. Be patient... :grin:


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OfflineSpudz76
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Re: Simple question regarding B+ strain on WBS. [Re: ]
    #2999665 - 08/13/04 10:25 AM (12 years, 8 months ago)

B+ also can be funny at times. I have 6 jars of that and none show jack and it's been 3 weeks, but the other two strains I did at the same time (and same substrate mixture, which was too wet) are pretty much as done as they are going to be (again, too wet, so they went but won't make it to 100% - but at least they did something!).

You could, if you have 1cc or so of spores left, try a liquid culture method (karo/honey/malt/whatever) and see if you get germination on that, and then reinnoculate with a live mycelium syringe to "kick them in the ass" and get them going. At least you'd be waiting on two different things then and maybe it won't drive you nuts as much (I know I would go crazy if I wasn't trying ten different things at once).


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Offlinedreamsxclovers
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Re: Simple question regarding B+ strain on WBS. [Re: Anonymous]
    #11352213 - 10/30/09 04:08 PM (7 years, 5 months ago)

I have inoculated 1 syringe of B+ in 8 jars. today is the 10th day and there are 4 jars with dime sized- half dollar sized growth.  the first sign was after 5 days.
i still have 4 jars to go, so not having any growth in just 2 jars after 8 days isn't that big of a deal in my eyes.

this strain is VERY forgiving.
the only thing it cant handle is too much heat.. like did you sue a pressure cooker? if so, did you wait at least 24 hours before opening it. the only way you would show NO GROWTH is if
A. it got too hot
B. the spores didn't make it into the jars
C. you arn't keeping it at 75-82 degrees in a dark place

REMEMBER: HEAT KILLS SPORES, COLD STUNTS THEIR GROWTH TILL IT REACHES THE RIGHT TEMP
anything else you could have done wrong would result in contamination.

HOPE THAT HELPS.


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Invisibledaytripper05
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Re: Simple question regarding B+ strain on WBS. [Re: dreamsxclovers]
    #11352292 - 10/30/09 04:22 PM (7 years, 5 months ago)

hahaha...this thread is 5 years old by someone who wasn't even a member. :rofl:


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OfflineAmbience
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Re: Simple question regarding B+ strain on WBS. [Re: daytripper05]
    #11352311 - 10/30/09 04:25 PM (7 years, 5 months ago)

haha he made an account just to post that too.

btw early congrats on 3 year mark daytripper


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Offlinegornyhuy
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Re: Simple question regarding B+ strain on WBS. [Re: Ambience]
    #11352354 - 10/30/09 04:32 PM (7 years, 5 months ago)

I'm guessing anonymous has been watching this thread like a hawk for 5 years and FINALLY got some answers.


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Offlinephototronicnubius
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Re: Simple question regarding B+ strain on WBS. [Re: gornyhuy]
    #11352779 - 10/30/09 05:49 PM (7 years, 5 months ago)

Be patient. Some strains are slower than others. If you don't see any growth by 2 weeks. I might be worried.


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