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OfflineMycofriend
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Is this a Contaminated Syringe???
    #2996738 - 08/12/04 07:26 PM (12 years, 3 months ago)

3 of 10 syringes looked like this that I received from a vendor. The spores are clumped together and appear to be held together by something. Are these bad? Hopefully I will have attached a photo for refernce.



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OfflineSuntzu
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Re: Is this a Contaminated Syringe??? [Re: Mycofriend]
    #2996967 - 08/12/04 08:10 PM (12 years, 3 months ago)

Hmm.  Tough to say.  Large clumps like that are suspicious, but could be just fine.  If you shake it up, will they disperse?  There's only one real way to find out for sure if they're contaminated  :wink:  Comparing performance to the regular-looking syringes should be good enough, but a negative control would be recommended.  Good luck


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OfflineMycofriend
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Re: Is this a Contaminated Syringe??? [Re: Suntzu]
    #2997011 - 08/12/04 08:17 PM (12 years, 3 months ago)

When you shake it up the ball just bounces around... wont't disperse.


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OfflineSuntzu
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Re: Is this a Contaminated Syringe??? [Re: Mycofriend]
    #2997056 - 08/12/04 08:23 PM (12 years, 3 months ago)

That does sound like something binding the clump together. . .doesn't sound good, but still worth a shot.


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OfflinePolecat
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Re: Is this a Contaminated Syringe??? [Re: Suntzu]
    #2997159 - 08/12/04 08:52 PM (12 years, 3 months ago)

humm
same opinion here... once had a syr with clumped spores with no disperson when shaked. turned out well.

separetly experiment a bad and a good syringe(triplicate) on agar, honey, whatever. you should arrive to some conclusions this way


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O Caos é uma Ordem por decifrar.
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Offlinemycoguy
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Re: Is this a Contaminated Syringe??? [Re: Mycofriend]
    #3000429 - 08/13/04 01:33 PM (12 years, 3 months ago)

are you shaking the syring, while theres some air in it? You won't be able to break up that clump, if there's no air in the syringe.

Put the needle inside a flame, and suck up some hot air. Then shake.


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OfflineSuntzu
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Re: Is this a Contaminated Syringe??? [Re: mycoguy]
    #3002296 - 08/13/04 10:32 PM (12 years, 3 months ago)

Looks like a little air bubble in the pic. . .Very good advice, though;  More air might give better shaking.  I like the idea of sucking air from a flame
[do it all the time actually :wink: ]


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OfflineMycofriend
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Re: Is this a Contaminated Syringe??? [Re: Suntzu]
    #3002806 - 08/14/04 12:54 AM (12 years, 3 months ago)

I put some on a dozen anti-bacterial agar jars and it is some of the weakest, cottony looking stuff I've ever seen. once it fully infects the plate I'll take a picture of it. If those are somehow spores still, they are crap and weak.


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OfflinePoontangaroo
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Re: Is this a Contaminated Syringe??? [Re: Mycofriend]
    #3003054 - 08/14/04 02:13 AM (12 years, 3 months ago)

A couple of my most recent syringes had the exact same deal, clump-like matter amidst the liquid. Although as one of my recent posts seemed to come to the conclusion that i might have had a contaminated syringe, 2 of the other 3 have worked out nicely so far. Although I really did do my best to keep sterilization the key, apparently my efforts werent good enough with a bunch of my jars and this one syringe caused me a lot of trouble.
Do I think that a clump in the syringe means u should be worried? Probably not... I think my troubles were most likely my own fault somewhere down the line... after all, these clumps came in all the syringes i recently bought and i got an assload (24 pints... that's a lot right?) of nicely colonized jars.
In conclusion, the klumps were nice people, they might have needed to lay off the complex carbohydrates, but u cant fault a person for loving the pasta with extra cheese, wordemup to the homies.


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i like to tap larger bottoms because they ripple better and its sorta mesmorizing


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