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OfflineHerbanShaman
I am the Walrus

Registered: 07/15/04
Posts: 233
Loc: Everywhere and Nowhere
Last seen: 11 years, 6 months
Possibly contaminated spore prints - need advice
    #2996214 - 08/12/04 05:21 PM (13 years, 2 months ago)

I've been thinking about trying to make a karo jar using a spore print that i saved from former batch. Problem is that this print might not be totally sterile. Here's how it happened. I took a playing card wiped it off, put the mushroom on it, and covered it with a glass to prevent dust. No I did not spray the card with alcohol, a mistake. I happened to be starting a trip when I made the print, I had realized it was then or never (it was my last mushie). The print looked great when it was done. I wrappped it in aluminum foil and put it away at room temp. Can I do the karo tek and then maybe smell the mixture to detect contamination. Will I be able to tell visually if its contaminated? What do you guys suggest, should I just break down and buy a spore syringe to be safe and try the karo tek then?


--------------------
"There is a world beyond ours, a world that is far away, nearby, and invisible. And there is where God lives, where the dead live, the spirits and the saints, a world where everything has already happened and everything is known. That world talks. It has a language of its own. I report what it says. The sacred mushroom takes me by the hand and brings me to the world where everything is known. It is they, the sacred mushrooms, that speak in a way I can understand. I ask them and they answer me. When I return from the trip that I have taken with them, I tell what they have told me and what they have shown me."
-- Mazatec shaman


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OfflineQandA
wanderer

Registered: 05/19/04
Posts: 352
Last seen: 13 years, 2 months
Re: Possibly contaminated spore prints - need advice [Re: HerbanShaman]
    #2996225 - 08/12/04 05:25 PM (13 years, 2 months ago)

I'd at least try the karo tek with it. Alot of times you can see contams in karo/honey. If you had some agar, that would be best though.


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I'm not crazy...crazy.


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InvisibleFucknuckle
Dog Lover

Registered: 04/24/04
Posts: 6,762
Re: Possibly contaminated spore prints - need advice [Re: HerbanShaman]
    #2996247 - 08/12/04 05:29 PM (13 years, 2 months ago)

Dude you should have just made the print on foil.


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What it is, is what it is my Brother.
It is as it is, so suffer thru it.


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OfflineQandA
wanderer

Registered: 05/19/04
Posts: 352
Last seen: 13 years, 2 months
Re: Possibly contaminated spore prints - need advice [Re: Fucknuckle]
    #2996316 - 08/12/04 05:47 PM (13 years, 2 months ago)

The problem with foil is that is causes the spores to clump together. Paper removes moisture from the spores so that they will not clump. Though, to some ppl, it doesnt matter.... depends on whether you are making syringes or not.


--------------------
I'm not crazy...crazy.


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Invisiblemycopsycho
Tit Inspector.
Male
Folding@home Statistics
Registered: 06/17/04
Posts: 3,712
Loc: Going Nowhere Fast
Re: Possibly contaminated spore prints - need advice [Re: HerbanShaman]
    #2996318 - 08/12/04 05:48 PM (13 years, 2 months ago)

ok, you can save it, but it's gonna take a little work...... make a liquid media jar and nocc that bitch up. once you see myc growth make another liquid media jar and get it ready. make a water/peroxide mixture (10:1 ratio) fill a syringe about half way with the water/peroxide mix and then suck some myc up and shoot the cocktail into the new liquid media jar. it should grow contaminate free now! also, be patient with the second jar, the peroxide will most likely shock the myc at first so give it an extra day or two.

:peace:


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I Am The Sickness.

Diploid: I think adults have a right to make stupid decisions and it's nobody else's fucking business.


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OfflineMAGnum
veteran

Registered: 07/08/04
Posts: 2,421
Last seen: 5 years, 10 months
Re: Possibly contaminated spore prints - need advice [Re: Fucknuckle]
    #2996352 - 08/12/04 05:56 PM (13 years, 2 months ago)

Start a culture on agar, start a shitload.

Some will probably contam, it's pretty much trial and error.

Once the shit grows, Isolate it onto new petri dishes.

If they all have mold, definately don't isolate it if it has cobweb.

This is a standard isolation tech for any type of isolation, It's so simple school children could do it.

Goodluck


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Offlinemeanoldman
old guy
Registered: 06/14/04
Posts: 85
Last seen: 13 years, 29 days
Re: Possibly contaminated spore prints - need advice [Re: HerbanShaman]
    #2996455 - 08/12/04 06:30 PM (13 years, 2 months ago)

If agar and Petrie dishes are not your cup of tea, there is another possibility.

Make a bunch of PF jars. Inoculate them with as LITTLE spore water as possible. Hopefully at least one injection site in one jar will be clean, take a sample of myc from there and do a transfer.

Sucks, but if you have no other spores....


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