Home | Community | Message Board


Mycohaus
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop for: ½ Pint Jars, Agar, Petri Dish, Rye Grain

Jump to first unread post. Pages: 1
Invisiblepaddoholland
addict

Registered: 02/14/01
Posts: 317
Loc: the Netherlands
Re: Slow colonisation
    #299500 - 04/24/01 02:34 AM (15 years, 7 months ago)

Okay, some more info, sorry,
720 ml jars with 175 grams of rye and 200 ml water were pc'ed 1 hour.
These jars were shaken while hot and cooled down. After this they were innoculated with agar chunks from a fully colonised petri-dish.
One agar chunk/jar
Colonised in the dark, at 18?C (I know it's a little low).
After one week 1 jar had a little mycelium growth, others do not show any growth. Jars were thouroughly shaken when agar chunks were in the jar. In the first week they also have been shaken a few times to spread the non-visible mycelium!

I have now a heating mat, so the jars are getting at 20-25?C.
I hope this will speed up colonisation!
More to come........

---------------------------------------------------------------------------
Rolling, smoking!!!



--------------------
---------------------------------------------------------------------------
Visit & Support Free Spore Ring Europe



Post Extras: Print Post  Remind Me! Notify Moderator
Offlinehatter
addict
Registered: 03/27/01
Posts: 501
Last seen: 14 years, 5 months
Re: Slow colonisation [Re: paddoholland]
    #300220 - 04/24/01 02:40 AM (15 years, 7 months ago)

Could you translate Celsius to Fahrenheit for us Americanos?



--------------------
How is a raven like a writing desk?

How deep is the rabbit hole? To find out, go.to/FreeSporeRing


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblepaddoholland
addict

Registered: 02/14/01
Posts: 317
Loc: the Netherlands
Re: Slow colonisation [Re: hatter]
    #300226 - 04/24/01 02:46 AM (15 years, 7 months ago)

That's very hard for me, I can only say, 20?C is room temperature, and 37?C is body temp.
Dunno about the farenheit, but maybe this helps, wait I'll just look it up at haslta vista...just a sec

---------------------------------------------------------------------------
Rolling, smoking!!!



--------------------
---------------------------------------------------------------------------
Visit & Support Free Spore Ring Europe



Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblepaddoholland
addict

Registered: 02/14/01
Posts: 317
Loc: the Netherlands
Re: Slow colonisation [Re: hatter]
    #300231 - 04/24/01 02:50 AM (15 years, 7 months ago)

Found it:To convert ?F to ?C
( Farenheit to Celcius )
Substract 32,
multiply by 5 and
then divide by 9

--------------------------------------------------------------------------------

To convert ?C to ?F
( Celcius to Farenheit )
multiply by 9,
divide by 5 and
then add 32
so 18?C = 64.4 F
20-25?C = 68-77 F
Better?



---------------------------------------------------------------------------
Rolling, smoking!!!



--------------------
---------------------------------------------------------------------------
Visit & Support Free Spore Ring Europe



Post Extras: Print Post  Remind Me! Notify Moderator
Offlinehatter
addict
Registered: 03/27/01
Posts: 501
Last seen: 14 years, 5 months
Re: Slow colonisation [Re: paddoholland]
    #300285 - 04/24/01 04:38 AM (15 years, 7 months ago)

Well, if you were spawned off over a week ago and nothing has happened, my first instinct is something unseen happened(maybe bacteria contam..).

However, given the temperature conversions, your heat is still a bit too low...82-86F(no idea what that is C) is optimal for mycelial growth. I personally find the hot water heater closet to be the perfect temperature(85F).

Give it a few more days before you throw in the towel, and shake the HELL out of those jars one last time!

Good Luck!



--------------------
How is a raven like a writing desk?

How deep is the rabbit hole? To find out, go.to/FreeSporeRing


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblepaddoholland
addict

Registered: 02/14/01
Posts: 317
Loc: the Netherlands
Re: Slow colonisation [Re: hatter]
    #300295 - 04/24/01 05:10 AM (15 years, 7 months ago)

Yeah, heat is much 2 low.
Therefor I have bought a heating mat.
Hope it will help!

---------------------------------------------------------------------------
Rolling, smoking!!!



--------------------
---------------------------------------------------------------------------
Visit & Support Free Spore Ring Europe



Post Extras: Print Post  Remind Me! Notify Moderator
Offlinegreymule
enthusiast
Registered: 03/25/01
Posts: 135
Loc: Colorado
Last seen: 5 years, 6 months
Re: Slow colonisation [Re: paddoholland]
    #300318 - 04/24/01 07:15 AM (15 years, 7 months ago)

Whether the agar itself is continuing to grow mycellium seems to be the biggest clue here and it's still missing. Look at the agar slices and see if the cut sides and the bottom where no mycellium were present when you innoculated now have mycellium growing in those spots. The agar usually colonizes itself first before branching out to new food. The delay may be caused in part by the "new food" phenom.

Them not busy bein' born are busy dyin'.


--------------------
Them not busy bein' born are busy dyin'.


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblepaddoholland
addict

Registered: 02/14/01
Posts: 317
Loc: the Netherlands
Re: Slow colonisation [Re: greymule]
    #300325 - 04/24/01 07:33 AM (15 years, 7 months ago)

The mycelium in the petri-dishes has grown onto the surface where once agar had it's place, the chunks in the jars are not visible due to the shaking. Only one chunk is visible and from there no signs of growth are visible!
Will post new findings at the end of the week, maybe next week!

---------------------------------------------------------------------------
Rolling, smoking!!!



--------------------
---------------------------------------------------------------------------
Visit & Support Free Spore Ring Europe



Post Extras: Print Post  Remind Me! Notify Moderator
Offlinegreymule
enthusiast
Registered: 03/25/01
Posts: 135
Loc: Colorado
Last seen: 5 years, 6 months
Re: Slow colonisation [Re: paddoholland]
    #300327 - 04/24/01 07:39 AM (15 years, 7 months ago)

Sounds like a temp prob, then. Let us know.

Them not busy bein' born are busy dyin'.


--------------------
Them not busy bein' born are busy dyin'.


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblepaddoholland
addict

Registered: 02/14/01
Posts: 317
Loc: the Netherlands
Re: Slow colonisation [Re: greymule]
    #300330 - 04/24/01 07:45 AM (15 years, 7 months ago)

Will do, end of week, maybe monday I hope to have fully colonised jars!

---------------------------------------------------------------------------
Rolling, smoking!!!



--------------------
---------------------------------------------------------------------------
Visit & Support Free Spore Ring Europe



Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblepaddoholland
addict

Registered: 02/14/01
Posts: 317
Loc: the Netherlands
Re: Slow colonisation [Re: greymule]
    #301368 - 04/25/01 07:03 AM (15 years, 7 months ago)

After putting jars on a heating mat colonisation appeared very quick. Throughout the whole jar white mycelium growth is visible.
So this was a temperature problem SOLVED!!!
Thanks for the help guys/girls, pictures from previous flushes will be posted next week!

---------------------------------------------------------------------------
Rolling, smoking!!!



--------------------
---------------------------------------------------------------------------
Visit & Support Free Spore Ring Europe



Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop for: ½ Pint Jars, Agar, Petri Dish, Rye Grain

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* Slow colonization (I searched first) flower_child 1,977 10 10/17/06 07:17 PM
by creamcorn
* Slow colonization of PF jars grayswan 2,118 7 12/08/04 02:00 PM
by blissedout
* slow colonizing jars... tonyperez420 3,189 6 11/06/06 11:28 AM
by BooNanZa
* Mad slow colonization camplo 2,262 3 08/16/08 02:44 PM
by camplo
* Slow colonization of birdseed allowing too much time for... Ayrios 1,061 5 09/08/04 02:52 PM
by Prisoner#1
* Slow colonization.
( 1 2 all )
u4ia 2,679 23 05/13/03 08:14 PM
by u4ia
* slow colonization means slow pinning/fruiting? wds 1,687 2 06/08/04 12:03 AM
by jong21
* Slow colonization, whats the deal?
( 1 2 all )
tokey666 1,870 20 09/08/05 10:30 PM
by gema

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Magash, Shroomism, george castanza, Prisoner#1, RogerRabbit, Citric, total, FooMan, 13shrooms, stonesun, EvilMushroom666, cronicr, PussyFart
934 topic views. 18 members, 92 guests and 26 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Gaiana.nl
Please support our sponsors.

Copyright 1997-2016 Mind Media. Some rights reserved.

Generated in 0.056 seconds spending 0.003 seconds on 14 queries.