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OfflineLocoJRP
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Registered: 07/28/04
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Reinoculation?
    #2992204 - 08/11/04 07:22 PM (13 years, 1 month ago)

If after 2 weeks with no signs of growth or contamination, should my friend reinoculate his 12 pf jars with a new spore syringe? He used one 10 cc cambodian syringe for all 12 1/2 pint jars from Mike O'Logical's.
:confused:


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Invisiblemycopsycho
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Re: Reinoculation? [Re: LocoJRP]
    #2992223 - 08/11/04 07:25 PM (13 years, 1 month ago)

if you got the jars from mike then you know it's gotta be the syringe or your incubator cause mikes jars are the shit! what temp are you keeping them at?


--------------------
I Am The Sickness.

Diploid: I think adults have a right to make stupid decisions and it's nobody else's fucking business.


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OfflineLocoJRP
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Re: Reinoculation? [Re: mycopsycho]
    #2992258 - 08/11/04 07:34 PM (13 years, 1 month ago)

The question is just hypothetical. The jars were just inoculated on 08/09/2004 at 9pm and there is no growth yet today, which I know is not expected. But I was just wandering if I should reinoculate if that were to happen. The jars are in a rubbermaid covered under blankets. There is no heating equipment in the rubber maid but the thermistat in the house is set to 82.


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Invisiblemycopsycho
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Re: Reinoculation? [Re: LocoJRP]
    #2992426 - 08/11/04 08:07 PM (13 years, 1 month ago)

it is important that you know what the temp is in the rubbermaid.


--------------------
I Am The Sickness.

Diploid: I think adults have a right to make stupid decisions and it's nobody else's fucking business.


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OfflineLocoJRP
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Registered: 07/28/04
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Re: Reinoculation? [Re: mycopsycho]
    #2992508 - 08/11/04 08:20 PM (13 years, 1 month ago)

I was thinking that the temp in the rubbermaid should be similar to room temperature. I'll pick up a thermometer tomorrow. Any affordable recomendations?


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Invisiblemycopsycho
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Re: Reinoculation? [Re: LocoJRP]
    #2992526 - 08/11/04 08:24 PM (13 years, 1 month ago)

wally world has some thermometers for like 10 bucks. btw, you do know that your cakes will be about 1-2 degrees(F) warmer than the air temp, don't you?


--------------------
I Am The Sickness.

Diploid: I think adults have a right to make stupid decisions and it's nobody else's fucking business.


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OfflineLocoJRP
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Posts: 41
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Re: Reinoculation? [Re: mycopsycho]
    #2992579 - 08/11/04 08:31 PM (13 years, 1 month ago)

I think I read that the myc will give off some heat, but I don't have any growth yet. Temps at around 85 degrees should cause the spores to germinate quicker, right?


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Invisiblemycopsycho
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Re: Reinoculation? [Re: LocoJRP]
    #2992610 - 08/11/04 08:36 PM (13 years, 1 month ago)

85 is pretty optimal. keep it there until you see growth and then drop it 1 degree.


--------------------
I Am The Sickness.

Diploid: I think adults have a right to make stupid decisions and it's nobody else's fucking business.


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OfflineLocoJRP
newbie
Registered: 07/28/04
Posts: 41
Last seen: 12 years, 6 months
Re: Reinoculation? [Re: mycopsycho]
    #2992631 - 08/11/04 08:39 PM (13 years, 1 month ago)

Cool, thanks for the advice.


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Invisiblemycopsycho
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Re: Reinoculation? [Re: LocoJRP]
    #2992651 - 08/11/04 08:43 PM (13 years, 1 month ago)

no problem. that's what this board is here for, to help newcomers!


--------------------
I Am The Sickness.

Diploid: I think adults have a right to make stupid decisions and it's nobody else's fucking business.


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Amazon Shop: ½ Pint Jars

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