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InvisibleGnuBobo
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Karo innoc. with a bit of colonized grain?
    #2976326 - 08/08/04 03:27 AM (18 years, 3 months ago)

Hey OMC. I was reading this afternoon through past Karo tek posts, but didn't find an answer to my question. I have a few rye jars that are colonized. I'm going to G2G some of them, and wondered if I could put a small chunk or two of myc. from these jars into a karo mix? Anyone tried it?

Thanks!


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Jerry Garcia. JERRY GARCIA! JERRY GARCIA!!!!


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Invisiblemycopsycho
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Re: Karo innoc. with a bit of colonized grain? [Re: GnuBobo]
    #2976429 - 08/08/04 04:19 AM (18 years, 3 months ago)

you are asking for trouble . that method has a better chance of contams.IMO


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I Am The Sickness.

Diploid: I think adults have a right to make stupid decisions and it's nobody else's fucking business.


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InvisibleGnuBobo
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Re: Karo innoc. with a bit of colonized grain? [Re: mycopsycho]
    #2976452 - 08/08/04 04:24 AM (18 years, 3 months ago)

Hmmm. So, in the tek it says you can use a bit of living mushroom tissue or a spore syringe.
The approach would then to be to get a spore print, mix with water, then shoot into Karo?
I saw a post by another member regarding mycellium under a microscope, that the sample had a few contams on it, but these were no trouble as the myc. had colonized the substrate.
So, assuming there's some level of contamination on the mycellium in a grain jar, it would colonize and take over the karo jar before the myc got a foothold?
Thanks!

GB


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Jerry Garcia. JERRY GARCIA! JERRY GARCIA!!!!


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OfflineTheGanjaGorilla
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Re: Karo innoc. with a bit of colonized grain? [Re: GnuBobo]
    #2976611 - 08/08/04 05:45 AM (18 years, 3 months ago)

Sure you can do that. The trick is to keep everything as clean as you can while you work. I lucked out once after a BRF jar went bad. I just dropped the chunk of cake into the karo mix, and it grew from there...

It's best of you use a floow hood though...


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"Whateva I do what I want..." Eric Catman




"Whateva, I do what I want..." Eric Cartman


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InvisibleMykey
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Re: Karo innoc. with a bit of colonized grain? [Re: GnuBobo]
    #2976750 - 08/08/04 07:01 AM (18 years, 3 months ago)

If you are going to try it a quick dunk in h2o2/water solution will reduce the likelyhood of contams. Just put a little in a shotglass and dunk the piece in it before you put it in the karo water.

Good Luck!


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InvisibleGnuBobo
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Re: Karo innoc. with a bit of colonized grain? [Re: Mykey]
    #2976839 - 08/08/04 07:26 AM (18 years, 3 months ago)

Interesting. Thanks. Are we talking 3% H2o2 in a 10% solution in water or should the ratio be higher for this dunk?
Cheers man.

GB


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Jerry Garcia. JERRY GARCIA! JERRY GARCIA!!!!


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InvisibleMykey
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Re: Karo innoc. with a bit of colonized grain? [Re: GnuBobo]
    #2976900 - 08/08/04 07:42 AM (18 years, 3 months ago)

I think a bit stronger is best for this type of thing. I would go with about 30 to 40% h2o2(3%). I know it goes without saying,but a glovebox in combination with the h2o2 treatment is the best way to go. If you have to do it in the open air make sure you have taken every precaution possible to sterilize the transfer area and wear a mask so you dont breath any bacteria onto your work. Lysol makes a good air sanitizer that will go a long way to helping to neutralize the contams in the area if you do the transfer in open air. Its called oust and it is a much finer mist and is actually able to cling to airborne contamination where just spraying lysol aroung does virtually nothing because the mist is just too heavy to attach itself to anything as small as airborne contams.


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InvisibleGnuBobo
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Re: Karo innoc. with a bit of colonized grain? [Re: Mykey]
    #2977325 - 08/08/04 10:07 AM (18 years, 3 months ago)

    Yeah, I've got some Oust (Ozium is far more expensive, eh?)  and lysol, and rubbing alcohol.  Plus, I have a glovebox that's from a tek on mycotopia. 
      30-40% H2o2?  All right.  At least the stuff is cheap.  :grin:
      By the way, if you read this, what are your experiences with Oust like?  My grow chamber is hidden in some cabinets.  Essentially, I have a "double wall"  to use.  I open the cabninet, spray with lysol, then vacuum to fan it after 15 minutes.  So, a few days ago, I bought some Oust.  I'm just wondering how it works given when we move, we create air flow.  How "sealed" does the room have to be for that stuff to work?  I'm just unsure how much I should trust it, outside of a glovebox or a small bathroom.  Thanks, man.

GB


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Jerry Garcia. JERRY GARCIA! JERRY GARCIA!!!!


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Offlinescatmanrav
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Re: Karo innoc. with a bit of colonized grain? [Re: GnuBobo]
    #2977775 - 08/08/04 06:23 PM (18 years, 3 months ago)

Why should it be any harder then a GTG transfer from one jar to another? Havnt had any problems doing these using no flowhood/glovebox taking my normal sterilization precautions (heavy procautions). I would imagine it would work fine. Just try and get a very small peice of white and keep out any grain not covered in white (once you break up the substrate some of it gets scraped off exposing the grain, I've found this to be a possible problem once).

Good luck.


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"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


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Invisiblemycopsycho
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Re: Karo innoc. with a bit of colonized grain? [Re: GnuBobo]
    #2978069 - 08/08/04 08:22 PM (18 years, 3 months ago)

i was just thinking.... if you had access to any you could include some antibiotics to the karo/water and then it would be safe for a live culture transfer.


--------------------
I Am The Sickness.

Diploid: I think adults have a right to make stupid decisions and it's nobody else's fucking business.


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InvisibleGnuBobo
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Re: Karo innoc. with a bit of colonized grain? [Re: mycopsycho]
    #2978154 - 08/08/04 09:01 PM (18 years, 3 months ago)

Antibiotics? Hmmm. I do have a lot of doxycline lying around. But this would only help against bacteria, rigtht? I've run into a bit of green and some cobweb before....these aren't bacteria though, are they? How would one go about mixing up a solution of antibiotics? Anyone know?
Well, thanks for all the help, people! I'll give it a shot with a few jars and report back. THanks!

GB


--------------------
Jerry Garcia. JERRY GARCIA! JERRY GARCIA!!!!


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