Home | Community | Message Board


Lil Shop Of Spores
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop for: ½ Pint Jars

Jump to first unread post. Pages: 1
InvisibleMykey
spectraltraveler

Registered: 04/07/04
Posts: 542
Easy Liquid Growth Medium!
    #2975030 - 08/07/04 11:54 AM (12 years, 4 months ago)

  I thought I would post a super easy method of making a kick ass jar of liquid myc using a ready make sterile medium that I have yet to see anyone post anything about.


You can get this stuff at your local Tractor Supply or feed store and it is usually right next to the syringes and antibiotics.



This stuff is awesome!! It comes sterile with a self sealing port on the top for drawing out what you need. PERFECT!


Here is an easy way of setting up your jar.

1. Prepare the lid of your jar by punching a hole in it with a nail and placing a nice blob of high temp silicone on the hole and letting it cure for a few hrs.

2. PC the jar for about twenty minutes at 15psi (empty) and then remove it to your glovebox or otherwise sterile area.

3. With a sterile syringe (that you picked up while you were buying this dextrose solution) draw out enough of the solution to fill the jar about half way full and inject it through the self sealing port you made with the nail and silicone. (it is a good idea to swab the ports with alcohol before drawing and injecting just to be safe.)

4. Now just sterilize your spore syringe needle and inject a little bit of the solution into the jar and slap that baby into the inccubator and wait a while and you have got some very healthy myc that will go a long way toward colonizing many jars with a quickness!

Here is a jar done this way:

Well thats it!
It doesnt get much easier than that!
** by the way when the solution is all gone you have a very nice pc'able bottle with a self sealing port which can be used for storing sterile water for making spore syringes and many other things!** :goodluck:


--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinescatmanrav
Brainy Smurf

Registered: 05/08/04
Posts: 11,483
Loc: Flag
Last seen: 3 years, 10 months
Trusted Cultivator
Re: Easy Liquid Growth Medium! [Re: Mykey]
    #2975069 - 08/07/04 12:15 PM (12 years, 4 months ago)

Couldn't you just inject some spores into that container then suck it out as you needed? Seems like you transfered it to a clean jar but nothing else which seems like a waste of energy to me over just making the whole jug of the stuff myc? Maybe I missed something or maybe this is so you dont have to use the whole thing at once but I would imagine even if you used the whole thing it would store in the fridge well.


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblemycopsycho
Tit Inspector.
Male
Folding@home Statistics
Registered: 06/17/04
Posts: 3,712
Loc: Going Nowhere Fast
Re: Easy Liquid Growth Medium! [Re: Mykey]
    #2975119 - 08/07/04 01:02 PM (12 years, 4 months ago)

how much did that dextrose cost? i tend to see better growth with malt.


--------------------
I Am The Sickness.

Diploid: I think adults have a right to make stupid decisions and it's nobody else's fucking business.


Post Extras: Print Post  Remind Me! Notify Moderator
InvisiblePsyllyMe
LOST.......
Registered: 07/25/04
Posts: 848
Loc: Center of the Known Unive...
Re: Easy Liquid Growth Medium! [Re: Mykey]
    #2975128 - 08/07/04 01:09 PM (12 years, 4 months ago)

I too was curious why you didn't just innoculate the "jug"......

Its only 500ml.......

peace


Post Extras: Print Post  Remind Me! Notify Moderator
Offlineevilchipmunk
All your nutsare belong tome.

Registered: 05/13/04
Posts: 268
Last seen: 9 years, 3 months
Re: Easy Liquid Growth Medium! [Re: PsyllyMe]
    #2975156 - 08/07/04 01:32 PM (12 years, 4 months ago)

Probably because that bottle is a bit opaque, hard to see myc (or contams, or anything else) growing in there. Just a guess. :P


--------------------
George Dubya Bush's Resume.


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleMykey
spectraltraveler

Registered: 04/07/04
Posts: 542
Re: Easy Liquid Growth Medium! [Re: evilchipmunk]
    #2975303 - 08/07/04 02:39 PM (12 years, 4 months ago)

Yeah,the jug is very opaque and it would be impossible to tell what kind of growth you were getting. Also 500mil is way more than you need for a batch of liquid myc,anywhere from 100mils to 250mils seems to be a good amount. The other problem is that there isnt enough air inside the container to sustain healthy growth. Filling a half pint or a pint jar about half full leaves enough air inside to grow out the myc. Trust me guys at like three bucks for a bottle of this stuff you really cant go wrong. They also sell a variety of syringes and needles for about 1.75 a five pack!


--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblewoodzmun
listening tonature
Registered: 07/14/04
Posts: 341
Re: Easy Liquid Growth Medium! *DELETED* [Re: Mykey]
    #2975699 - 08/07/04 04:37 PM (12 years, 4 months ago)

Post deleted by woodzmun


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleMykey
spectraltraveler

Registered: 04/07/04
Posts: 542
Re: Easy Liquid Growth Medium! [Re: woodzmun]
    #2976792 - 08/08/04 12:12 AM (12 years, 4 months ago)

You can also just suck some of the dex solution into your spore syringe once it is used down to the last half cc or even less. As long as you flame the needle first you should get nice little mini batch going inside the syringe that will inocculate many more jars.
No jars to sterilize or anything,it doesnt get much more simple than that!
Just a thought. :tripping:


--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop for: ½ Pint Jars

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* Elementary question regarding Liquid Growth Medium Stix Russell 697 3 02/27/04 01:45 PM
by MilkVein
* Liquid Growth Medium northernshroom 364 2 05/13/09 07:31 PM
by RogerRabbit
* Q about Liquid Growth Medium Tek kid_alamo 732 2 08/09/02 01:45 PM
by kid_alamo
* Liquid Growth Medium Mitchnast 1,432 15 05/25/05 07:53 PM
by deanofmean
* liquid growth medium 1uvu 791 7 02/17/05 04:16 PM
by midway
* Q about Liquid Growth Medium Tek kid_alamo 806 3 08/10/02 09:41 AM
by kid_alamo
* Liquid Growth Medium.. acadian 582 5 12/14/05 10:06 PM
by Rahz
* Liquid Growth Medium Ainasko 569 4 05/15/03 02:27 PM
by Ainasko

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Magash, Shroomism, george castanza, Prisoner#1, RogerRabbit, Citric, total, FooMan, 13shrooms, stonesun, EvilMushroom666, cronicr, PussyFart
818 topic views. 20 members, 79 guests and 19 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Azarius
Please support our sponsors.

Copyright 1997-2016 Mind Media. Some rights reserved.

Generated in 0.044 seconds spending 0.004 seconds on 14 queries.