Home | Community | Message Board


FreeSpores.com
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop for: ½ Pint Jars, Agar, Malt Extract

Jump to first unread post. Pages: 1 | 2 | Next >  [ show all ]
Invisiblemycopsycho
Tit Inspector.
Male
Folding@home Statistics
Registered: 06/17/04
Posts: 3,712
Loc: Going Nowhere Fast
malt jar!!!!
    #2973685 - 08/06/04 10:51 PM (12 years, 4 months ago)

liquid myc culture

1- procure a half-pint jar w/ lid & ring and also a spore syringe.
2- drill/punch a hole in the center of the lid large enough for the syringe.
3- mix 3/4 cup of water with 1/2 tsp.(2.5 ml.) malt.
4- put lid & ring on and put aluminum foil over the top of that and then Pressure Cook (PC) for 20 min. @ 15 psi. , when finished let cool to below 90 degrees F.
5- once cool, setup your sterile environment (flowhood, glove box or even your oven will work) with an alcohol lamp, extra alcohol in a dish, paper towels/napkins, small round bandaid and lastly the spore syringe.
6- remove the foil from the jar, shake the needle and then sterilize the point with the alcohol lamp (get it red hot), cool the needle with a wipe & some alcohol and then inject 1-2 cc/mil spore solution into the jar.
7- once injected wipe the needle w/alcohol and put the guard back on it.
8- cover injection site w/ round bandaid (thanks for that tip magash!).
9- put the jars in your incubator set at 82-84 degrees F. for 4-14 days, you should notice growth by then.
10- sterilize empty syringes in your PC for 15-20 @ 15 psi.
11- remove the bandaid from the jar, sterilize your needle again and then suck myc solution into empty syringe(s) for use on PF style jars or substrate bags.

this can also be used for cloning. step 10 says empty syringes but if you want to make your jar perpetual then you can fill your syringes with karo water (i say karo cause malt might plug it... not sure though) and then sterilize so when you use them you inject the sugar mix into the jar and then draw myc water out thus sustaining the myc food supply.


and that's it. hope everyone likes this.


--------------------
I Am The Sickness.

Diploid: I think adults have a right to make stupid decisions and it's nobody else's fucking business.


Edited by IAmTheSickness (08/07/04 01:15 PM)


Post Extras: Print Post  Remind Me! Notify Moderator
InvisiblePsyllyMe
LOST.......
Registered: 07/25/04
Posts: 848
Loc: Center of the Known Unive...
Re: malt jar!!!! [Re: mycopsycho]
    #2973723 - 08/06/04 11:06 PM (12 years, 4 months ago)

Well Myco......I am tryin out a Sierra Nevada Jar........

Yes i have too much time and too many spores.......

I did add a pincho honey.......

we'll see.......

peace


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblemycopsycho
Tit Inspector.
Male
Folding@home Statistics
Registered: 06/17/04
Posts: 3,712
Loc: Going Nowhere Fast
Re: malt jar!!!! [Re: PsyllyMe]
    #2974715 - 08/07/04 06:47 AM (12 years, 4 months ago)

post your results!


--------------------
I Am The Sickness.

Diploid: I think adults have a right to make stupid decisions and it's nobody else's fucking business.


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblemycopsycho
Tit Inspector.
Male
Folding@home Statistics
Registered: 06/17/04
Posts: 3,712
Loc: Going Nowhere Fast
Re: malt jar!!!! [Re: mycopsycho]
    #2977568 - 08/08/04 08:11 AM (12 years, 4 months ago)

so.... anyone put this tek to use yet? if so tell us what your results were.


--------------------
I Am The Sickness.

Diploid: I think adults have a right to make stupid decisions and it's nobody else's fucking business.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineAnnoA
Experimenter
 User Gallery

Folding@home Statistics
Registered: 06/18/99
Posts: 24,121
Loc: my room
Last seen: 2 months, 14 days
Re: malt jar!!!! [Re: mycopsycho]
    #2977580 - 08/08/04 08:23 AM (12 years, 4 months ago)

This is the karo tek with malt extract. i don't see why it would work any differently.
You are still left with the same problems one of them is that you can't easily spot contamination.


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblemycopsycho
Tit Inspector.
Male
Folding@home Statistics
Registered: 06/17/04
Posts: 3,712
Loc: Going Nowhere Fast
Re: malt jar!!!! [Re: Anno]
    #2977584 - 08/08/04 08:33 AM (12 years, 4 months ago)

i've seen karo and malt side by side and malt is fater. as for the contams, that isn't so much of a problem if you practice proper sterile techniques.


--------------------
I Am The Sickness.

Diploid: I think adults have a right to make stupid decisions and it's nobody else's fucking business.


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibleboxtop703
ThesaurusLinguae Graecae
 User Gallery

Registered: 06/26/01
Posts: 5,011
Re: malt jar!!!! [Re: mycopsycho]
    #2977586 - 08/08/04 08:36 AM (12 years, 4 months ago)

Quote:

i've seen karo and malt side by side and malt is fater




Do you mean "faster" or "fatter"?

What do you mean?

Fater?


Edited by boxtop703 (08/08/04 08:42 AM)


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblemycopsycho
Tit Inspector.
Male
Folding@home Statistics
Registered: 06/17/04
Posts: 3,712
Loc: Going Nowhere Fast
Re: malt jar!!!! [Re: boxtop703]
    #2977590 - 08/08/04 08:42 AM (12 years, 4 months ago)

oh shit, sorry, trying to type real fast. i meant faster, malt is deffinately a catalyst for growth.


--------------------
I Am The Sickness.

Diploid: I think adults have a right to make stupid decisions and it's nobody else's fucking business.


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblefastfred
Old Hand
Male User Gallery

Folding@home Statistics
Registered: 05/17/04
Posts: 6,899
Loc: Dark side of the moon
Re: malt jar!!!! [Re: mycopsycho]
    #2977618 - 08/08/04 09:09 AM (12 years, 4 months ago)

Quote:

mycopsycho said:
i've seen karo and malt side by side and malt is faster. as for the contams, that isn't so much of a problem if you practice proper sterile techniques.




Yea, it seems pretty obvious that malt is far superior to any kind of sugarwater/honey technique.

It never ceases to amaze me how many people are willing to use inferior media simply out of laziness.


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblemycopsycho
Tit Inspector.
Male
Folding@home Statistics
Registered: 06/17/04
Posts: 3,712
Loc: Going Nowhere Fast
Re: malt jar!!!! [Re: fastfred]
    #2977807 - 08/08/04 11:44 AM (12 years, 4 months ago)

yes, laziness in the fact that malt can be harder to find, other than that the only bad thing about malt is the higher the concentration the cloudier the water will get. but if you are patient then the water will clear a little as the myc grows.


--------------------
I Am The Sickness.

Diploid: I think adults have a right to make stupid decisions and it's nobody else's fucking business.


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleBi0TeK
elephant man

Registered: 11/07/02
Posts: 3,002
Loc: Yorkshire Moors, Great Br...
Re: malt jar!!!! [Re: fastfred]
    #2977831 - 08/08/04 11:55 AM (12 years, 4 months ago)

Quote:

fastfred said:
It never ceases to amaze me how many people are willing to use inferior media simply out of laziness.




Agreed. Thats why I use agar  :smirk:


--------------------
PROMOTE BACTERIA. THEY'RE THE ONLY CULTURE SOME PEOPLE HAVE.


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblemycopsycho
Tit Inspector.
Male
Folding@home Statistics
Registered: 06/17/04
Posts: 3,712
Loc: Going Nowhere Fast
Re: malt jar!!!! [Re: Bi0TeK]
    #2978062 - 08/08/04 01:18 PM (12 years, 4 months ago)

a malt jar is pretty much the same thing as agar, it's just a liquid medium whereas agar is a solid medium. you could even go as far as to make an antibiotic malt jar that you could use to transfer myc to. hmmm.... i like that idea, i've got my next project now.


--------------------
I Am The Sickness.

Diploid: I think adults have a right to make stupid decisions and it's nobody else's fucking business.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineKonkeyDong
newbie
Registered: 07/08/04
Posts: 45
Last seen: 12 years, 3 months
Re: malt jar!!!! [Re: Bi0TeK]
    #2978066 - 08/08/04 01:21 PM (12 years, 4 months ago)

I like liquid media. I use malt from a homebrew store online. It cost me 16 bucks for 3 lbs. I use 4 grams with 96 ml of water. 1lb= roughly 454 grams, so I won't have to buy any more malt for the next 10 years.

You can call it being cheap, and you can call it laziness if you want, but I have successfully used this tek many times and I am proud of my results.

I am interested in Agar, but I am still reading up on it. Any advice you can give me about what kinds of agar/nutr. mixes you use would be appreciated.

Until I graduate to agar, I will be sticking with malt. It's fast, it works great, and it's CHEAP. I am experimenting with h2o2 in liquid spawn and non-sterile respawning and I am having a great time with it.
My goal is to be able to use about a pint of liquid spawn to start a culture of 3+ non sterile gallons in a 5 gallon bucket. Then I would like to distribute that bucket all over my yard.

I believe that even the newest of newbies can contribute ideas and new methods of doing things. Trying new methods is a hell of lot more rewarding than regurgitating information written by somebody else (that experimented with new things).

So you can call me lazy, you can call me cheap, but if I get this to work, I'll be doing the shroom dance in your momma's underpants.

peace out.


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblemycopsycho
Tit Inspector.
Male
Folding@home Statistics
Registered: 06/17/04
Posts: 3,712
Loc: Going Nowhere Fast
Re: malt jar!!!! [Re: KonkeyDong]
    #2978075 - 08/08/04 01:25 PM (12 years, 4 months ago)

^^^^^props^^^^^ stay out of peeps moms undies though, you never know what kind of contams are lurking!:grin:


--------------------
I Am The Sickness.

Diploid: I think adults have a right to make stupid decisions and it's nobody else's fucking business.


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleBi0TeK
elephant man

Registered: 11/07/02
Posts: 3,002
Loc: Yorkshire Moors, Great Br...
Re: malt jar!!!! [Re: mycopsycho]
    #2978113 - 08/08/04 01:41 PM (12 years, 4 months ago)

Quote:

mycopsycho said:
a malt jar is pretty much the same thing as agar




It most definitely is not! :smirk:

Quote:

KonkeyDong said:
It's fast, it works great, and it's CHEAP.




Agar, if bought from a Asian supermarket, is fast, works MUCH better and is as cheap as chips  :smirk:


--------------------
PROMOTE BACTERIA. THEY'RE THE ONLY CULTURE SOME PEOPLE HAVE.


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblemycopsycho
Tit Inspector.
Male
Folding@home Statistics
Registered: 06/17/04
Posts: 3,712
Loc: Going Nowhere Fast
Re: malt jar!!!! [Re: Bi0TeK]
    #2978126 - 08/08/04 01:47 PM (12 years, 4 months ago)

It most definitely is not!

sure it is, except for isolation purposes. why is everyone having such trouble letting go of their security blankets?


--------------------
I Am The Sickness.

Diploid: I think adults have a right to make stupid decisions and it's nobody else's fucking business.


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblefastfred
Old Hand
Male User Gallery

Folding@home Statistics
Registered: 05/17/04
Posts: 6,899
Loc: Dark side of the moon
Re: malt jar!!!! [Re: KonkeyDong]
    #2978198 - 08/08/04 02:22 PM (12 years, 4 months ago)

Quote:

KonkeyDong said:
You can call it being cheap, and you can call it laziness if you want, but I have successfully used this tek many times and I am proud of my results.
<snip>
So you can call me lazy, you can call me cheap, but if I get this to work, I'll be doing the shroom dance in your momma's underpants.
peace out.




You need to go back and reread my post. I'm dissing sugarwater/honeywater not malt.

The main problem with everyone using their own nutty media formulas is that you can't compare anything! I've seen recipes calling for anywhere from the standard ammounts to nearly straight malt syrup.

There is a very steep point of diminishing returns as far as "proprietary super formula" vs. standardized media.

-FF


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblemycopsycho
Tit Inspector.
Male
Folding@home Statistics
Registered: 06/17/04
Posts: 3,712
Loc: Going Nowhere Fast
Re: malt jar!!!! [Re: fastfred]
    #2978748 - 08/08/04 06:08 PM (12 years, 4 months ago)

we are nowhere near the "super formula" yet. so, would anyone like to add to this?


--------------------
I Am The Sickness.

Diploid: I think adults have a right to make stupid decisions and it's nobody else's fucking business.


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblemycopsycho
Tit Inspector.
Male
Folding@home Statistics
Registered: 06/17/04
Posts: 3,712
Loc: Going Nowhere Fast
Re: malt jar!!!! [Re: mycopsycho]
    #2980830 - 08/09/04 10:41 AM (12 years, 4 months ago)

i ordered some pharm grade caffeine. once i get it i'm going to add a pinch of it to a malt jar then nocc it up and see how that works.


--------------------
I Am The Sickness.

Diploid: I think adults have a right to make stupid decisions and it's nobody else's fucking business.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineKaptKid
Spaced Pirate
Male User Gallery

Registered: 12/11/03
Posts: 6,247
Loc: Bright Side of the Sun
Last seen: 1 day, 17 hours
Re: malt jar!!!! [Re: mycopsycho]
    #2980869 - 08/09/04 11:02 AM (12 years, 4 months ago)

This tek looks good.I have not luck with liquid culture in the past but now thatI've started doing g2g it's time to learn that tek.
Will give this a try.

Thanks

:captain:


--------------------
Child of the 60's, Tripping ever since.


:sun:


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1 | 2 | Next >  [ show all ]

Amazon Shop for: ½ Pint Jars, Agar, Malt Extract

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* Liquid malt dealy Toricious 422 2 08/21/04 10:32 PM
by liveby
* malt growth / timeline deviation 340 1 08/23/04 01:25 PM
by mycopsycho
* How Many People Love Malt and Dextrose LC's but
( 1 2 3 all )
HippieChick 2,869 41 06/20/05 02:45 PM
by Roadkill
* bandaids for gas exchange
( 1 2 all )
hinestomato 1,812 21 12/02/08 03:30 PM
by Ogre812
* wbs jar prep help desoul420 324 5 05/06/09 07:03 PM
by Carcass
* Bandaids instead of Tyvek for Rye/Cracked Corn? hollowellreid 670 5 01/31/06 07:14 PM
by Snaggletooth
* filter jars? jape 780 14 12/21/07 12:46 PM
by lostinautumn7
* Dextrose / Malt Liquid Culture Sudden Cloudyness, Contamination!? Pics Defconx3 3,607 9 02/13/07 05:50 PM
by Defconx3

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Magash, Shroomism, george castanza, Prisoner#1, RogerRabbit, Citric, total, FooMan, 13shrooms, stonesun, EvilMushroom666, cronicr, PussyFart
1,833 topic views. 25 members, 110 guests and 27 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Azarius
Please support our sponsors.

Copyright 1997-2016 Mind Media. Some rights reserved.

Generated in 0.074 seconds spending 0.006 seconds on 16 queries.