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OfflineToricious
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Registered: 09/27/03
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agar vs karo
    #2971480 - 08/06/04 11:46 AM (13 years, 9 months ago)

Hi, i've recently been experimenting with agar. It looked pretty easy and I thought ide experiment with inexpensive fresh storebought mushrooms to give me a lot of practice and experimenting without it costing me an arm and a leg and having the stress of growing a more expensive mushroom. It's been going alright, but I also recently heard about karo - which looks better in every and any ways. I've just been wondering if experimenting with agar isnt a waste of time when there is a better alternative out there and I am also wondering if agar has any sort of advantage over karo? Thanks :laugh:


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OfflineAnnoA
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Re: agar vs karo [Re: Toricious]
    #2971587 - 08/06/04 12:22 PM (13 years, 9 months ago)

You can't see and isolate contaminations in liquid cultures. Agar has a flat, 2 dimensional surface and you can spot contaminations or different patterns in growth.


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Invisibledog
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Re: agar vs karo [Re: Toricious]
    #2971606 - 08/06/04 12:28 PM (13 years, 9 months ago)

I prefer peroxidated agar by far. A karo mycelial solution is handy to inoculate bunch of jars quickly, but g2g transfers accomplish the same thing. A contaminted Karo jar can be difficult to spot. With agar you can not only easily identify a contaminated plate, you can also isolate a clean sample from it.


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OfflineToricious
Theblunt-smokinglense-man.

Registered: 09/27/03
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Re: agar vs karo [Re: Anno]
    #2973649 - 08/06/04 10:35 PM (13 years, 9 months ago)

thanks :smile: - so karo is kind of like gambling - it could blow up in your face and cost you a lot in grain?


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Offlinehyphae
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Re: agar vs karo [Re: Toricious]
    #2973662 - 08/06/04 10:41 PM (13 years, 9 months ago)

Yes, but if your sterile technique is good and it should be then it will work awesome as long as the syringe you use is not contamed. I've never had contamed karo myself :laugh:


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Invisiblemycopsycho
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Re: agar vs karo [Re: hyphae]
    #2973701 - 08/06/04 10:56 PM (13 years, 9 months ago)

karo is too damn easy!


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