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OfflineCyber
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Registered: 06/14/04
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Cloning question!
    #2967227 - 08/05/04 08:44 AM (19 years, 7 months ago)

Will cloning a fruit get you similar results to agar isolation.

In respect to growth and flush.

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OfflineAnnoA
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Re: Cloning question! [Re: Cyber]
    #2967747 - 08/05/04 12:09 PM (19 years, 7 months ago)

The cloned mycelium will have the same characteristics as the mushroom you cloned it from.

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OfflineCyber
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Re: Cloning question! [Re: Anno]
    #2967762 - 08/05/04 12:13 PM (19 years, 7 months ago)

So you are saying pick the best looking one. :smile:

So is it better to multispore to agar and isolate a good substrain or to clone a nice fruit?

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OfflineaNewPerception
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Registered: 05/16/04
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Re: Cloning question! [Re: Cyber]
    #2967935 - 08/05/04 01:03 PM (19 years, 7 months ago)

clone, would be a step ahead of the multispore-isolate. cloning IS isolating.

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Offlinehyphae
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Re: Cloning question! [Re: Cyber]
    #2968744 - 08/05/04 03:52 PM (19 years, 7 months ago)

If possible always try to clone the biggest in a group as opposed to a single monster as they tend to give better yields. GL

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InvisibleATWAR
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Registered: 01/26/03
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Re: Cloning question! [Re: hyphae]
    #2970632 - 08/06/04 12:12 AM (19 years, 7 months ago)

In an ideal situation one would test several isolates no matter if they come from clones or are true multispore isolates. You cannot predict their fruiting characteristics accuratly until grown out "in isolation".


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InvisibleSubGen1us

Registered: 11/26/02
Posts: 3,427
Re: Cloning question! [Re: ATWAR]
    #2970931 - 08/06/04 03:00 AM (19 years, 7 months ago)

So would u say clone the same fruit in 10 petris.
find the faster growing, lets say 4 of the plates.
then grow those 4 plates and and see witch has a more even pinset.
then get a print then do a multispore on 10 plates then pick the faster growing
and so on?

Ive been wanting to start but have never truley understood what to do.
I was just going to experiment with the above process or something like that
if it makes sence.


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Offlinehyphae
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Re: Cloning question! [Re: SubGen1us]
    #2972080 - 08/06/04 01:14 PM (19 years, 7 months ago)

No SubGen1us that would be wasting your time bigtime. What you want is to take samples from more than one mushroom thats it plain and simple, the mushrooms will be almost identical to that one but how prolific it fruits is what needs to be determined. In general cloning from groups will give clones that pin more prolifically than single clones. Mushrooms are isolates in themselves BTW (FACT) :wink: You will have to growout that single monster too see if it has an acceptable pin count which it may or may not. I hope this clears up any confusion. :wink: GL

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